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| Main Authors: | , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Animals : an open access journal from MDPI
2025
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| Online Access: | https://pubmed.ncbi.nlm.nih.gov/40646746/ |
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| _version_ | 1868266179230957568 |
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| author | Sohn, Min-Young Kang, Gyoungsik Kim, Kyung-Ho Son, Ha-Jeong Park, Chan-Il |
| author_facet | Sohn, Min-Young Kang, Gyoungsik Kim, Kyung-Ho Son, Ha-Jeong Park, Chan-Il Sohn, Min-Young Kang, Gyoungsik Kim, Kyung-Ho Son, Ha-Jeong Park, Chan-Il |
| collection | PubMed - marine biology |
| contents | Molecular Adjuvant Potential of and in Starry Flounder Challenged with . Sohn, Min-Young Kang, Gyoungsik Kim, Kyung-Ho Son, Ha-Jeong Park, Chan-Il In fish, the innate immune system is crucial for rapid defense against pathogens. In this study, we performed transcriptome sequencing using next-generation sequencing (NGS) to identify and characterize granulocyte colony-stimulating factor () and macrophage colony-stimulating factor () in starry flounder (). The gene (594 bp, 198 aa) features a conserved IL-6 domain, while the gene (621 bp, 207 aa) contains a predicted transmembrane region. Phylogenetic analysis confirmed high evolutionary conservation with other marine species. Quantitative real-time PCR revealed that is highly expressed in the skin, peripheral blood leukocytes, and muscle, with significant up-regulation in immune organs following infection; exhibited a similar tissue-specific expression pattern. Recombinant () was produced using a cell-free system and effectively enhanced leukocyte phagocytic activity at an optimal concentration of 150 μg/mL, without causing cytotoxicity in hemolytic assays. In contrast, exhibited folding issues during purification. These findings highlight the potential of as a molecular adjuvant to enhance immune responses in aquaculture. This study provides foundational knowledge for developing cytokine-based adjuvants, which could reduce antibiotic dependency and enhance vaccine efficacy in sustainable aquaculture systems. |
| format | Artículo científico |
| id | pubmed_40646746 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Animals : an open access journal from MDPI |
| record_format | pubmed |
| spellingShingle | Molecular Adjuvant Potential of and in Starry Flounder Challenged with . Sohn, Min-Young Kang, Gyoungsik Kim, Kyung-Ho Son, Ha-Jeong Park, Chan-Il Molecular Adjuvant Potential of and in Starry Flounder Challenged with . Sohn, Min-Young Kang, Gyoungsik Kim, Kyung-Ho Son, Ha-Jeong Park, Chan-Il In fish, the innate immune system is crucial for rapid defense against pathogens. In this study, we performed transcriptome sequencing using next-generation sequencing (NGS) to identify and characterize granulocyte colony-stimulating factor () and macrophage colony-stimulating factor () in starry flounder (). The gene (594 bp, 198 aa) features a conserved IL-6 domain, while the gene (621 bp, 207 aa) contains a predicted transmembrane region. Phylogenetic analysis confirmed high evolutionary conservation with other marine species. Quantitative real-time PCR revealed that is highly expressed in the skin, peripheral blood leukocytes, and muscle, with significant up-regulation in immune organs following infection; exhibited a similar tissue-specific expression pattern. Recombinant () was produced using a cell-free system and effectively enhanced leukocyte phagocytic activity at an optimal concentration of 150 μg/mL, without causing cytotoxicity in hemolytic assays. In contrast, exhibited folding issues during purification. These findings highlight the potential of as a molecular adjuvant to enhance immune responses in aquaculture. This study provides foundational knowledge for developing cytokine-based adjuvants, which could reduce antibiotic dependency and enhance vaccine efficacy in sustainable aquaculture systems. |
| title | Molecular Adjuvant Potential of and in Starry Flounder Challenged with . |
| url | https://pubmed.ncbi.nlm.nih.gov/40646746/ |