Microfluidic Fabrication of Hyaluronic Acid-Cysteine-Modified Chitosan Microspheres for CD44-Targeted Probiotic Delivery in Colitis Treatment.
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| Format: | Artículo científico |
| Langue: | en |
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ACS applied materials & interfaces
2025
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| author | Qiao, Fengzhi Luo, Ya Lei, Peng Wang, Shaolei Duan, Zhi Cui, Hongchang Liu, Tongjie Yi, Huaxi De Souza, Cristabelle Xuan, Guanhua Mao, Xiangzhao Ma, Zhao Zhang, Lanwei Lin, Kai |
| author_facet | Qiao, Fengzhi Luo, Ya Lei, Peng Wang, Shaolei Duan, Zhi Cui, Hongchang Liu, Tongjie Yi, Huaxi De Souza, Cristabelle Xuan, Guanhua Mao, Xiangzhao Ma, Zhao Zhang, Lanwei Lin, Kai Qiao, Fengzhi Luo, Ya Lei, Peng Wang, Shaolei Duan, Zhi Cui, Hongchang Liu, Tongjie Yi, Huaxi De Souza, Cristabelle Xuan, Guanhua Mao, Xiangzhao Ma, Zhao Zhang, Lanwei Lin, Kai |
| collection | PubMed - marine biology |
| contents | Microfluidic Fabrication of Hyaluronic Acid-Cysteine-Modified Chitosan Microspheres for CD44-Targeted Probiotic Delivery in Colitis Treatment. Qiao, Fengzhi Luo, Ya Lei, Peng Wang, Shaolei Duan, Zhi Cui, Hongchang Liu, Tongjie Yi, Huaxi De Souza, Cristabelle Xuan, Guanhua Mao, Xiangzhao Ma, Zhao Zhang, Lanwei Lin, Kai Hyaluronic Acid Chitosan Animals Colitis Probiotics Humans Hyaluronan Receptors Mice Caco-2 Cells Microspheres Cysteine Dextran Sulfate Bifidobacterium bifidum Drug Delivery Systems Gastrointestinal Microbiome Mice, Inbred C57BL Probiotics play a crucial role in regulating intestinal immune homeostasis and supporting gut health; however, oral administration faces challenges such as nonspecific distribution and low efficacy. To achieve precise and efficient delivery, in this study, a targeted delivery system embedded probiotics to the colonic inflammatory site CD44 was constructed by covalently linking yaluronic acid (HA) to ysteine-modified hitosan (CCH) using microfluidic technology. Two probiotic strains, FL-276.1 and ATCC 19398, were encapsulated within the modified chitosan matrix to form probiotics@CCH microspheres (MSs), with an average diameter of approximately 276 μm. Based on the receptor-ligand binding mechanism of HA and CD44, combined with the intestinal mucosal adhesion properties conferred by cysteine-modified chitosan, the probiotics@CCH MSs exhibited a high capture rate for inflammatory Caco-2 cells and demonstrated prolonged retention and targeted localization in a DSS-induced colitis mouse model. Furthermore, probiotics@CCH MSs contributed to maintaining intestinal homeostasis by modulating gut microbiota composition, enhancing short-chain fatty acid production, and supporting the intestinal barrier integrity. The microfluidic-based delivery system facilitates the precise localization of probiotics within the intestine, providing a theoretical basis for enhancing probiotic applications in gut health management. |
| format | Artículo científico |
| id | pubmed_40651026 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | ACS applied materials & interfaces |
| record_format | pubmed |
| spellingShingle | Microfluidic Fabrication of Hyaluronic Acid-Cysteine-Modified Chitosan Microspheres for CD44-Targeted Probiotic Delivery in Colitis Treatment. Qiao, Fengzhi Luo, Ya Lei, Peng Wang, Shaolei Duan, Zhi Cui, Hongchang Liu, Tongjie Yi, Huaxi De Souza, Cristabelle Xuan, Guanhua Mao, Xiangzhao Ma, Zhao Zhang, Lanwei Lin, Kai Hyaluronic Acid Chitosan Animals Colitis Probiotics Humans Hyaluronan Receptors Mice Caco-2 Cells Microspheres Cysteine Dextran Sulfate Bifidobacterium bifidum Drug Delivery Systems Gastrointestinal Microbiome Mice, Inbred C57BL Microfluidic Fabrication of Hyaluronic Acid-Cysteine-Modified Chitosan Microspheres for CD44-Targeted Probiotic Delivery in Colitis Treatment. Qiao, Fengzhi Luo, Ya Lei, Peng Wang, Shaolei Duan, Zhi Cui, Hongchang Liu, Tongjie Yi, Huaxi De Souza, Cristabelle Xuan, Guanhua Mao, Xiangzhao Ma, Zhao Zhang, Lanwei Lin, Kai Hyaluronic Acid Chitosan Animals Colitis Probiotics Humans Hyaluronan Receptors Mice Caco-2 Cells Microspheres Cysteine Dextran Sulfate Bifidobacterium bifidum Drug Delivery Systems Gastrointestinal Microbiome Mice, Inbred C57BL Probiotics play a crucial role in regulating intestinal immune homeostasis and supporting gut health; however, oral administration faces challenges such as nonspecific distribution and low efficacy. To achieve precise and efficient delivery, in this study, a targeted delivery system embedded probiotics to the colonic inflammatory site CD44 was constructed by covalently linking yaluronic acid (HA) to ysteine-modified hitosan (CCH) using microfluidic technology. Two probiotic strains, FL-276.1 and ATCC 19398, were encapsulated within the modified chitosan matrix to form probiotics@CCH microspheres (MSs), with an average diameter of approximately 276 μm. Based on the receptor-ligand binding mechanism of HA and CD44, combined with the intestinal mucosal adhesion properties conferred by cysteine-modified chitosan, the probiotics@CCH MSs exhibited a high capture rate for inflammatory Caco-2 cells and demonstrated prolonged retention and targeted localization in a DSS-induced colitis mouse model. Furthermore, probiotics@CCH MSs contributed to maintaining intestinal homeostasis by modulating gut microbiota composition, enhancing short-chain fatty acid production, and supporting the intestinal barrier integrity. The microfluidic-based delivery system facilitates the precise localization of probiotics within the intestine, providing a theoretical basis for enhancing probiotic applications in gut health management. |
| title | Microfluidic Fabrication of Hyaluronic Acid-Cysteine-Modified Chitosan Microspheres for CD44-Targeted Probiotic Delivery in Colitis Treatment. |
| topic | Hyaluronic Acid Chitosan Animals Colitis Probiotics Humans Hyaluronan Receptors Mice Caco-2 Cells Microspheres Cysteine Dextran Sulfate Bifidobacterium bifidum Drug Delivery Systems Gastrointestinal Microbiome Mice, Inbred C57BL |
| url | https://pubmed.ncbi.nlm.nih.gov/40651026/ |