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| Format: | Artículo científico |
| Langue: | en |
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Veterinary immunology and immunopathology
2025
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| Accès en ligne: | https://pubmed.ncbi.nlm.nih.gov/40663881/ |
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| _version_ | 1868266176827621378 |
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| author | Nandhakumar Ramachandran, Ishwarya Elumalai, Preetham |
| author_facet | Nandhakumar Ramachandran, Ishwarya Elumalai, Preetham Nandhakumar Ramachandran, Ishwarya Elumalai, Preetham |
| collection | PubMed - marine biology |
| contents | Mucoadhesive chitosan-based nano vaccine as promising immersion vaccine against Edwardsiella tarda challenge in Nile tilapia (Oreochromis niloticus). Nandhakumar Ramachandran, Ishwarya Elumalai, Preetham Animals Chitosan Fish Diseases Cichlids Enterobacteriaceae Infections Bacterial Vaccines Edwardsiella tarda beta-Glucans Nanoparticles Immersion Vaccination Adjuvants, Immunologic Immunity, Mucosal Nanovaccines Immersion vaccination with a biomimetic mucoadhesive nanovaccine has been shown to induce a strong mucosal immune response against Edwardsiellosis, a serious bacterial disease in Nile tilapia caused by Edwardsiella tarda. This study aims to develop and investigate the efficacy of immersion delivery using a chitosan nano-vaccine (CS) with β-glucan (BG) as an adjuvant to enhance the mucoadhesive properties of the vaccine. The prepared vaccines were nano-sized and spherical as confirmed by scanning electron microscope (SEM), and the images show that nano vaccine greatly increased the binding and penetrating ability into gills when compared with formalin-killed vaccine. Fingerling-sized Nile tilapia (12 ± 2 g) were randomly assigned to four groups: control, Formalin Killed vaccine (Positive control), Chitosan/β-glucan alone (CS/BG), and Chitosan/β-glucan Vaccine (CS/BG V). Fish received immersion baths on days 1 and 21 with a 30-min booster dose. Samples were collected at two time intervals (14, and 28 days post-vaccination (Dpv)) to evaluate innate immune responses through lysozyme, myeloperoxidase, NBT, and superoxide dismutase activity were significantly elevated in vaccinated fish compared to the control group (p |
| format | Artículo científico |
| id | pubmed_40663881 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Veterinary immunology and immunopathology |
| record_format | pubmed |
| spellingShingle | Mucoadhesive chitosan-based nano vaccine as promising immersion vaccine against Edwardsiella tarda challenge in Nile tilapia (Oreochromis niloticus). Nandhakumar Ramachandran, Ishwarya Elumalai, Preetham Animals Chitosan Fish Diseases Cichlids Enterobacteriaceae Infections Bacterial Vaccines Edwardsiella tarda beta-Glucans Nanoparticles Immersion Vaccination Adjuvants, Immunologic Immunity, Mucosal Nanovaccines Mucoadhesive chitosan-based nano vaccine as promising immersion vaccine against Edwardsiella tarda challenge in Nile tilapia (Oreochromis niloticus). Nandhakumar Ramachandran, Ishwarya Elumalai, Preetham Animals Chitosan Fish Diseases Cichlids Enterobacteriaceae Infections Bacterial Vaccines Edwardsiella tarda beta-Glucans Nanoparticles Immersion Vaccination Adjuvants, Immunologic Immunity, Mucosal Nanovaccines Immersion vaccination with a biomimetic mucoadhesive nanovaccine has been shown to induce a strong mucosal immune response against Edwardsiellosis, a serious bacterial disease in Nile tilapia caused by Edwardsiella tarda. This study aims to develop and investigate the efficacy of immersion delivery using a chitosan nano-vaccine (CS) with β-glucan (BG) as an adjuvant to enhance the mucoadhesive properties of the vaccine. The prepared vaccines were nano-sized and spherical as confirmed by scanning electron microscope (SEM), and the images show that nano vaccine greatly increased the binding and penetrating ability into gills when compared with formalin-killed vaccine. Fingerling-sized Nile tilapia (12 ± 2 g) were randomly assigned to four groups: control, Formalin Killed vaccine (Positive control), Chitosan/β-glucan alone (CS/BG), and Chitosan/β-glucan Vaccine (CS/BG V). Fish received immersion baths on days 1 and 21 with a 30-min booster dose. Samples were collected at two time intervals (14, and 28 days post-vaccination (Dpv)) to evaluate innate immune responses through lysozyme, myeloperoxidase, NBT, and superoxide dismutase activity were significantly elevated in vaccinated fish compared to the control group (p |
| title | Mucoadhesive chitosan-based nano vaccine as promising immersion vaccine against Edwardsiella tarda challenge in Nile tilapia (Oreochromis niloticus). |
| topic | Animals Chitosan Fish Diseases Cichlids Enterobacteriaceae Infections Bacterial Vaccines Edwardsiella tarda beta-Glucans Nanoparticles Immersion Vaccination Adjuvants, Immunologic Immunity, Mucosal Nanovaccines |
| url | https://pubmed.ncbi.nlm.nih.gov/40663881/ |