CRISPR/Dx-based colorimetric and electrochemical detection systems for POCT applications.
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| Autores principales: | , , , , , , , , |
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| Formato: | Artículo científico |
| Lenguaje: | en |
| Publicado: |
Biosensors & bioelectronics
2025
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| _version_ | 1868266176827621376 |
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| author | Lau, Cia-Hin Liang, Qing-Le Chen, Xiaoqing Li, Jiahui Shi, Yi Huang, Yumei Xia, Qiyuan Zhu, Haibao Chen, Gang |
| author_facet | Lau, Cia-Hin Liang, Qing-Le Chen, Xiaoqing Li, Jiahui Shi, Yi Huang, Yumei Xia, Qiyuan Zhu, Haibao Chen, Gang Lau, Cia-Hin Liang, Qing-Le Chen, Xiaoqing Li, Jiahui Shi, Yi Huang, Yumei Xia, Qiyuan Zhu, Haibao Chen, Gang |
| collection | PubMed - marine biology |
| contents | CRISPR/Dx-based colorimetric and electrochemical detection systems for POCT applications. Lau, Cia-Hin Liang, Qing-Le Chen, Xiaoqing Li, Jiahui Shi, Yi Huang, Yumei Xia, Qiyuan Zhu, Haibao Chen, Gang Humans Colorimetry Biosensing Techniques Electrochemical Techniques Point-of-Care Testing CRISPR-Cas Systems Female Neoplasms Pregnancy Point-of-Care Systems To realize point-of-care testing (POCT) for human diseases, CRISPR/Cas technology has been integrated with commercial analytical devices and chromogenic substrates such as personal glucose meter (PGM), pregnancy test strips (PTS), and horseradish peroxidase (HRP). These integrated detection platforms enable portable diagnostics, economical, universal, rapid, and high throughput detection. The change in color and electrochemical signal enables visible signal readout and intuitive results without specialized equipment and skillful operators. Herein, we comprehensively and critically discuss these advancements and describe the molecular principles of each detection system. The current challenges and technical shortcomings associated with these CRISPR-based diagnostics for POCT applications are discussed. We also provide future perspectives and directions in this field that will improve the performance of CRISPR-PGM, CRISPR-PTS, and CRISPR-HRP for pathogens detection and molecular diagnostics. Their applications in early tumor detection and pan-cancer screening are highlighted. The potential ethical and societal impact of home self-tests for cancer are discussed. |
| format | Artículo científico |
| id | pubmed_40664139 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Biosensors & bioelectronics |
| record_format | pubmed |
| spellingShingle | CRISPR/Dx-based colorimetric and electrochemical detection systems for POCT applications. Lau, Cia-Hin Liang, Qing-Le Chen, Xiaoqing Li, Jiahui Shi, Yi Huang, Yumei Xia, Qiyuan Zhu, Haibao Chen, Gang Humans Colorimetry Biosensing Techniques Electrochemical Techniques Point-of-Care Testing CRISPR-Cas Systems Female Neoplasms Pregnancy Point-of-Care Systems CRISPR/Dx-based colorimetric and electrochemical detection systems for POCT applications. Lau, Cia-Hin Liang, Qing-Le Chen, Xiaoqing Li, Jiahui Shi, Yi Huang, Yumei Xia, Qiyuan Zhu, Haibao Chen, Gang Humans Colorimetry Biosensing Techniques Electrochemical Techniques Point-of-Care Testing CRISPR-Cas Systems Female Neoplasms Pregnancy Point-of-Care Systems To realize point-of-care testing (POCT) for human diseases, CRISPR/Cas technology has been integrated with commercial analytical devices and chromogenic substrates such as personal glucose meter (PGM), pregnancy test strips (PTS), and horseradish peroxidase (HRP). These integrated detection platforms enable portable diagnostics, economical, universal, rapid, and high throughput detection. The change in color and electrochemical signal enables visible signal readout and intuitive results without specialized equipment and skillful operators. Herein, we comprehensively and critically discuss these advancements and describe the molecular principles of each detection system. The current challenges and technical shortcomings associated with these CRISPR-based diagnostics for POCT applications are discussed. We also provide future perspectives and directions in this field that will improve the performance of CRISPR-PGM, CRISPR-PTS, and CRISPR-HRP for pathogens detection and molecular diagnostics. Their applications in early tumor detection and pan-cancer screening are highlighted. The potential ethical and societal impact of home self-tests for cancer are discussed. |
| title | CRISPR/Dx-based colorimetric and electrochemical detection systems for POCT applications. |
| topic | Humans Colorimetry Biosensing Techniques Electrochemical Techniques Point-of-Care Testing CRISPR-Cas Systems Female Neoplasms Pregnancy Point-of-Care Systems |
| url | https://pubmed.ncbi.nlm.nih.gov/40664139/ |