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| Format: | Artículo científico |
| Sprache: | en |
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Fish & shellfish immunology
2025
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| Schlagworte: | |
| Online-Zugang: | https://pubmed.ncbi.nlm.nih.gov/40669574/ |
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| _version_ | 1868266176820281345 |
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| author | Zhang, Hong Qing Cai, Long Wei Deng, Yu Hang Yan, Meng Xin Mu, Peng Fei Li, Bo Liu, Lan Hao Nie, P |
| author_facet | Zhang, Hong Qing Cai, Long Wei Deng, Yu Hang Yan, Meng Xin Mu, Peng Fei Li, Bo Liu, Lan Hao Nie, P Zhang, Hong Qing Cai, Long Wei Deng, Yu Hang Yan, Meng Xin Mu, Peng Fei Li, Bo Liu, Lan Hao Nie, P |
| collection | PubMed - marine biology |
| contents | Identification and functional characterization of cGAS and cGAS-like in snakehead Channa argus. Zhang, Hong Qing Cai, Long Wei Deng, Yu Hang Yan, Meng Xin Mu, Peng Fei Li, Bo Liu, Lan Hao Nie, P Fishes Animals Nucleotidyltransferases Fish Proteins Protein Domains Interferon Type I Gene Expression Regulation Signal Transduction Viperin Protein Immunity, Innate Aquaculture China Fish Diseases Cell Line Sequence Alignment Gene Expression Profiling In mammals, cyclic GMP-AMP synthase (cGAS) senses cytosolic dsDNA to trigger type I interferon (IFN) response via the sting signalling, but its regulatory role in fish remains unclear. In the present study, cGAS and cGAS-like (cGASL) were identified in the snakehead (Channa argus), but they differ in the organization of two domains, NTase domain, MAB21 domain, with an additional transmembrane domain in cGASL. It is further revealed that cGAS can bind with Sting to enhance the production of type I IFN and IFN-stimulated genes (ISGs), such as viperin, mx, and its entire molecule with all domains is necessary for its functioning. However, the co-transfection of cGAS or cGASL with tbk1 and mda5 inhibited the expression of ISGs in EPC cells. It is notable that cGAS may exhibit dual functionality either by promoting sting-dependent IFN response or by suppressing RLR pathway activation, whereas cGASL solely acted as an RLR pathway inhibitor. These findings reveal complexed, pathway-specific immunoregulatory roles of cGAS/cGASL in fish antiviral immunity. |
| format | Artículo científico |
| id | pubmed_40669574 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Fish & shellfish immunology |
| record_format | pubmed |
| spellingShingle | Identification and functional characterization of cGAS and cGAS-like in snakehead Channa argus. Zhang, Hong Qing Cai, Long Wei Deng, Yu Hang Yan, Meng Xin Mu, Peng Fei Li, Bo Liu, Lan Hao Nie, P Fishes Animals Nucleotidyltransferases Fish Proteins Protein Domains Interferon Type I Gene Expression Regulation Signal Transduction Viperin Protein Immunity, Innate Aquaculture China Fish Diseases Cell Line Sequence Alignment Gene Expression Profiling Identification and functional characterization of cGAS and cGAS-like in snakehead Channa argus. Zhang, Hong Qing Cai, Long Wei Deng, Yu Hang Yan, Meng Xin Mu, Peng Fei Li, Bo Liu, Lan Hao Nie, P Fishes Animals Nucleotidyltransferases Fish Proteins Protein Domains Interferon Type I Gene Expression Regulation Signal Transduction Viperin Protein Immunity, Innate Aquaculture China Fish Diseases Cell Line Sequence Alignment Gene Expression Profiling In mammals, cyclic GMP-AMP synthase (cGAS) senses cytosolic dsDNA to trigger type I interferon (IFN) response via the sting signalling, but its regulatory role in fish remains unclear. In the present study, cGAS and cGAS-like (cGASL) were identified in the snakehead (Channa argus), but they differ in the organization of two domains, NTase domain, MAB21 domain, with an additional transmembrane domain in cGASL. It is further revealed that cGAS can bind with Sting to enhance the production of type I IFN and IFN-stimulated genes (ISGs), such as viperin, mx, and its entire molecule with all domains is necessary for its functioning. However, the co-transfection of cGAS or cGASL with tbk1 and mda5 inhibited the expression of ISGs in EPC cells. It is notable that cGAS may exhibit dual functionality either by promoting sting-dependent IFN response or by suppressing RLR pathway activation, whereas cGASL solely acted as an RLR pathway inhibitor. These findings reveal complexed, pathway-specific immunoregulatory roles of cGAS/cGASL in fish antiviral immunity. |
| title | Identification and functional characterization of cGAS and cGAS-like in snakehead Channa argus. |
| topic | Fishes Animals Nucleotidyltransferases Fish Proteins Protein Domains Interferon Type I Gene Expression Regulation Signal Transduction Viperin Protein Immunity, Innate Aquaculture China Fish Diseases Cell Line Sequence Alignment Gene Expression Profiling |
| url | https://pubmed.ncbi.nlm.nih.gov/40669574/ |