Developing a molecular tool for evaluating the status of petrochemical-contaminated soils using functional gene ratio strategy.
Fuente:
PubMed
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Artículo científico |
| Language: | en |
| Published: |
Letters in applied microbiology
2025
|
| Subjects: | |
| Online Access: | |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1868266176813989889 |
|---|---|
| author | Ibrahim, Aliyu Dabai Rao, Juluri R Kulakov, Anna Dandare, Shamsudeen U Doherty, Rory Boyd, Mathew Allen, Christopher C R |
| author_facet | Ibrahim, Aliyu Dabai Rao, Juluri R Kulakov, Anna Dandare, Shamsudeen U Doherty, Rory Boyd, Mathew Allen, Christopher C R Ibrahim, Aliyu Dabai Rao, Juluri R Kulakov, Anna Dandare, Shamsudeen U Doherty, Rory Boyd, Mathew Allen, Christopher C R |
| collection | PubMed - marine biology |
| contents | Developing a molecular tool for evaluating the status of petrochemical-contaminated soils using functional gene ratio strategy. Ibrahim, Aliyu Dabai Rao, Juluri R Kulakov, Anna Dandare, Shamsudeen U Doherty, Rory Boyd, Mathew Allen, Christopher C R Soil Pollutants Soil Microbiology Environmental Monitoring Polycyclic Aromatic Hydrocarbons Dioxygenases Soil Gas Chromatography-Mass Spectrometry Petroleum Pollution Biodegradation, Environmental Petroleum Bacteria Metagenomics Petrochemical spills present a global environmental challenge, necessitating effective monitoring and remediation. This study was designed to develop a molecular tool using binary functional gene ratios to assess contamination levels and timelines in petrochemical-contaminated soils. Soils from petrochemical-contaminated sites were collected and hydrocarbon quantification was performed using gas chromatography-mass spectrometry and two-dimensional gas chromatography with flame ionization detection, against specific standards. Metagenomic DNA was extracted, and functional genes associated with hydrocarbon degradation [PAH Ring-hydroxylating dioxygenase (PAH-RHDα) and 6-oxocyclohex-1-ene-1-carbonyl-CoA hydrolase (bamA)] were analysed using quantitative PCR (qPCR). A diagnostic ratio of PAH degradation genes was applied to characterize pollution. In-silico gene mining was used to verify the functional gene ratios. MS revealed distinct contamination profiles correlating with pollution duration and pollutant levels. One set of samples had kerosene concentrations between 154 and 478.64 µg/g of soil, while the other contained |
| format | Artículo científico |
| id | pubmed_40674090 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Letters in applied microbiology |
| record_format | pubmed |
| spellingShingle | Developing a molecular tool for evaluating the status of petrochemical-contaminated soils using functional gene ratio strategy. Ibrahim, Aliyu Dabai Rao, Juluri R Kulakov, Anna Dandare, Shamsudeen U Doherty, Rory Boyd, Mathew Allen, Christopher C R Soil Pollutants Soil Microbiology Environmental Monitoring Polycyclic Aromatic Hydrocarbons Dioxygenases Soil Gas Chromatography-Mass Spectrometry Petroleum Pollution Biodegradation, Environmental Petroleum Bacteria Metagenomics Developing a molecular tool for evaluating the status of petrochemical-contaminated soils using functional gene ratio strategy. Ibrahim, Aliyu Dabai Rao, Juluri R Kulakov, Anna Dandare, Shamsudeen U Doherty, Rory Boyd, Mathew Allen, Christopher C R Soil Pollutants Soil Microbiology Environmental Monitoring Polycyclic Aromatic Hydrocarbons Dioxygenases Soil Gas Chromatography-Mass Spectrometry Petroleum Pollution Biodegradation, Environmental Petroleum Bacteria Metagenomics Petrochemical spills present a global environmental challenge, necessitating effective monitoring and remediation. This study was designed to develop a molecular tool using binary functional gene ratios to assess contamination levels and timelines in petrochemical-contaminated soils. Soils from petrochemical-contaminated sites were collected and hydrocarbon quantification was performed using gas chromatography-mass spectrometry and two-dimensional gas chromatography with flame ionization detection, against specific standards. Metagenomic DNA was extracted, and functional genes associated with hydrocarbon degradation [PAH Ring-hydroxylating dioxygenase (PAH-RHDα) and 6-oxocyclohex-1-ene-1-carbonyl-CoA hydrolase (bamA)] were analysed using quantitative PCR (qPCR). A diagnostic ratio of PAH degradation genes was applied to characterize pollution. In-silico gene mining was used to verify the functional gene ratios. MS revealed distinct contamination profiles correlating with pollution duration and pollutant levels. One set of samples had kerosene concentrations between 154 and 478.64 µg/g of soil, while the other contained |
| title | Developing a molecular tool for evaluating the status of petrochemical-contaminated soils using functional gene ratio strategy. |
| topic | Soil Pollutants Soil Microbiology Environmental Monitoring Polycyclic Aromatic Hydrocarbons Dioxygenases Soil Gas Chromatography-Mass Spectrometry Petroleum Pollution Biodegradation, Environmental Petroleum Bacteria Metagenomics |
| url | https://pubmed.ncbi.nlm.nih.gov/40674090/ |