Intraline genomic heterogeneity of the triple-negative breast cancer MDA-MB-231-luc-GFP cell line.
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| Auteurs principaux: | , , , , , , , , , |
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| Format: | Artículo científico |
| Langue: | en |
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Computational and structural biotechnology journal
2025
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| author | Varela-Rouco, Nair Estévez-Gómez, Nuria Fernández-Santiago, Cristóbal Tomás, Laura Pérez, Miriam García-Souto, Daniel Pasantes, Juan Jose Piñeiro, Roberto Alves, Joao M Posada, David |
| author_facet | Varela-Rouco, Nair Estévez-Gómez, Nuria Fernández-Santiago, Cristóbal Tomás, Laura Pérez, Miriam García-Souto, Daniel Pasantes, Juan Jose Piñeiro, Roberto Alves, Joao M Posada, David Varela-Rouco, Nair Estévez-Gómez, Nuria Fernández-Santiago, Cristóbal Tomás, Laura Pérez, Miriam García-Souto, Daniel Pasantes, Juan Jose Piñeiro, Roberto Alves, Joao M Posada, David |
| collection | PubMed - marine biology |
| contents | Intraline genomic heterogeneity of the triple-negative breast cancer MDA-MB-231-luc-GFP cell line. Varela-Rouco, Nair Estévez-Gómez, Nuria Fernández-Santiago, Cristóbal Tomás, Laura Pérez, Miriam García-Souto, Daniel Pasantes, Juan Jose Piñeiro, Roberto Alves, Joao M Posada, David Cancer cell lines are valuable models for studying tumor biology, yet their genomic evolution during culture can compromise experimental reproducibility. We conducted a detailed genomic analysis of the triple-negative breast cancer cell line MDA-MB-231-luc-GFP, examining sublines obtained from different sources, at various time points, and across distinct passages. We introduce the concept of intraline heterogeneity (ILH) to highlight the genomic variability observed among these sublines. Our analyses revealed extensive genomic diversity, including differences in single-nucleotide variants (SNVs) and copy number alterations (CNAs). In particular, CNAs exhibited remarkable heterogeneity, with pronounced chromosomal gains and losses between sublines, underscoring the impact of genomic instability on ILH. These findings suggest that ILH may influence experimental outcomes, emphasizing the importance of considering passage-specific genomic characterization to ensure consistency and reliability in cancer research. |
| format | Artículo científico |
| id | pubmed_40687988 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Computational and structural biotechnology journal |
| record_format | pubmed |
| spellingShingle | Intraline genomic heterogeneity of the triple-negative breast cancer MDA-MB-231-luc-GFP cell line. Varela-Rouco, Nair Estévez-Gómez, Nuria Fernández-Santiago, Cristóbal Tomás, Laura Pérez, Miriam García-Souto, Daniel Pasantes, Juan Jose Piñeiro, Roberto Alves, Joao M Posada, David Intraline genomic heterogeneity of the triple-negative breast cancer MDA-MB-231-luc-GFP cell line. Varela-Rouco, Nair Estévez-Gómez, Nuria Fernández-Santiago, Cristóbal Tomás, Laura Pérez, Miriam García-Souto, Daniel Pasantes, Juan Jose Piñeiro, Roberto Alves, Joao M Posada, David Cancer cell lines are valuable models for studying tumor biology, yet their genomic evolution during culture can compromise experimental reproducibility. We conducted a detailed genomic analysis of the triple-negative breast cancer cell line MDA-MB-231-luc-GFP, examining sublines obtained from different sources, at various time points, and across distinct passages. We introduce the concept of intraline heterogeneity (ILH) to highlight the genomic variability observed among these sublines. Our analyses revealed extensive genomic diversity, including differences in single-nucleotide variants (SNVs) and copy number alterations (CNAs). In particular, CNAs exhibited remarkable heterogeneity, with pronounced chromosomal gains and losses between sublines, underscoring the impact of genomic instability on ILH. These findings suggest that ILH may influence experimental outcomes, emphasizing the importance of considering passage-specific genomic characterization to ensure consistency and reliability in cancer research. |
| title | Intraline genomic heterogeneity of the triple-negative breast cancer MDA-MB-231-luc-GFP cell line. |
| url | https://pubmed.ncbi.nlm.nih.gov/40687988/ |