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Main Authors: Liu, Ge, Shan, Yeqi, Sun, Chaomin
Format: Artículo científico
Language:en
Published: FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2025
Subjects:
Online Access:https://pubmed.ncbi.nlm.nih.gov/40697044/
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author Liu, Ge
Shan, Yeqi
Sun, Chaomin
author_facet Liu, Ge
Shan, Yeqi
Sun, Chaomin
Liu, Ge
Shan, Yeqi
Sun, Chaomin
collection PubMed - marine biology
contents A Novel Exopolysaccharide, Highly Prevalent in Marine Spongiibacter, Triggers Pyroptosis to Exhibit Potent Anticancer Effects. Liu, Ge Shan, Yeqi Sun, Chaomin Pyroptosis Humans Animals Mice Antineoplastic Agents Polysaccharides, Bacterial THP-1 Cells Cell Line, Tumor Inflammasomes NLR Family, Pyrin Domain-Containing 3 Protein Pyroptosis is an inflammatory programmed cell death. In recent years, the potential of pyroptosis in tumor treatment has received widespread attention and has become a promising anti-tumor therapeutic strategy. EPS3.9 is a novel deep-sea bacterial exopolysaccharide that we obtained and has potent anti-tumor activity. EPS3.9 consisted of mannose and glucose with a molar ratio of 1:0.42. The average molecular weight of EPS3.9 was 17.1 kDa. EPS3.9 can induce lytic cell death in tumor cells. Mechanism analysis has revealed that it can directly target 5 membrane phospholipids and exert tumor cytotoxic activity through triggering NLRP3 inflammasome-mediated pyroptosis in the human monocytic leukemia THP-1 cells. EPS3.9 also has significant anti-tumor effects in Huh7.5 tumor-bearing mice and can activate anti-tumor immune responses. Besides, this active exopolysaccharide is ubiquitous among the genus Spongiibacter. This study provides an important theoretical basis for EPS3.9 as a new type of marine carbohydrate anti-tumor drug candidate and also provides scientific evidence for the feasibility and potential of tumor treatment by triggering pyroptosis.
format Artículo científico
id pubmed_40697044
institution PubMed
language en
publishDate 2025
publisher FASEB journal : official publication of the Federation of American Societies for Experimental Biology
record_format pubmed
spellingShingle A Novel Exopolysaccharide, Highly Prevalent in Marine Spongiibacter, Triggers Pyroptosis to Exhibit Potent Anticancer Effects.
Liu, Ge
Shan, Yeqi
Sun, Chaomin
Pyroptosis
Humans
Animals
Mice
Antineoplastic Agents
Polysaccharides, Bacterial
THP-1 Cells
Cell Line, Tumor
Inflammasomes
NLR Family, Pyrin Domain-Containing 3 Protein
A Novel Exopolysaccharide, Highly Prevalent in Marine Spongiibacter, Triggers Pyroptosis to Exhibit Potent Anticancer Effects. Liu, Ge Shan, Yeqi Sun, Chaomin Pyroptosis Humans Animals Mice Antineoplastic Agents Polysaccharides, Bacterial THP-1 Cells Cell Line, Tumor Inflammasomes NLR Family, Pyrin Domain-Containing 3 Protein Pyroptosis is an inflammatory programmed cell death. In recent years, the potential of pyroptosis in tumor treatment has received widespread attention and has become a promising anti-tumor therapeutic strategy. EPS3.9 is a novel deep-sea bacterial exopolysaccharide that we obtained and has potent anti-tumor activity. EPS3.9 consisted of mannose and glucose with a molar ratio of 1:0.42. The average molecular weight of EPS3.9 was 17.1 kDa. EPS3.9 can induce lytic cell death in tumor cells. Mechanism analysis has revealed that it can directly target 5 membrane phospholipids and exert tumor cytotoxic activity through triggering NLRP3 inflammasome-mediated pyroptosis in the human monocytic leukemia THP-1 cells. EPS3.9 also has significant anti-tumor effects in Huh7.5 tumor-bearing mice and can activate anti-tumor immune responses. Besides, this active exopolysaccharide is ubiquitous among the genus Spongiibacter. This study provides an important theoretical basis for EPS3.9 as a new type of marine carbohydrate anti-tumor drug candidate and also provides scientific evidence for the feasibility and potential of tumor treatment by triggering pyroptosis.
title A Novel Exopolysaccharide, Highly Prevalent in Marine Spongiibacter, Triggers Pyroptosis to Exhibit Potent Anticancer Effects.
topic Pyroptosis
Humans
Animals
Mice
Antineoplastic Agents
Polysaccharides, Bacterial
THP-1 Cells
Cell Line, Tumor
Inflammasomes
NLR Family, Pyrin Domain-Containing 3 Protein
url https://pubmed.ncbi.nlm.nih.gov/40697044/