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Hauptverfasser: Fan, Tzu-Pei, Lee, Jun-Ru, Lin, Che-Yi, Chen, Yi-Chih, Cutting, Ann E, Cameron, R Andrew, Yu, Jr-Kai, Su, Yi-Hsien
Format: Artículo científico
Sprache:en
Veröffentlicht: Science advances 2025
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Online-Zugang:https://pubmed.ncbi.nlm.nih.gov/40712008/
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author Fan, Tzu-Pei
Lee, Jun-Ru
Lin, Che-Yi
Chen, Yi-Chih
Cutting, Ann E
Cameron, R Andrew
Yu, Jr-Kai
Su, Yi-Hsien
author_facet Fan, Tzu-Pei
Lee, Jun-Ru
Lin, Che-Yi
Chen, Yi-Chih
Cutting, Ann E
Cameron, R Andrew
Yu, Jr-Kai
Su, Yi-Hsien
Fan, Tzu-Pei
Lee, Jun-Ru
Lin, Che-Yi
Chen, Yi-Chih
Cutting, Ann E
Cameron, R Andrew
Yu, Jr-Kai
Su, Yi-Hsien
collection PubMed - marine biology
contents Deep homology of a cis-regulatory syntax and the evolutionary origin of the notochord. Fan, Tzu-Pei Lee, Jun-Ru Lin, Che-Yi Chen, Yi-Chih Cutting, Ann E Cameron, R Andrew Yu, Jr-Kai Su, Yi-Hsien Notochord T-Box Domain Proteins Fetal Proteins Animals Brachyury Protein Gene Expression Regulation, Developmental Zebrafish Evolution, Molecular Enhancer Elements, Genetic Binding Sites Expression of in the notochord is regarded as a chordate novelty and links to the origin of the notochord, yet the evolution of this regulatory control remains unclear. Here, we uncovered a regulatory syntax (named SFZE) consisting of binding sites for four transcription factors in notochord enhancers of chordate genes. SFZE was also identified in potential enhancers in various non-chordate animals and even in , a unicellular relative to animals. These non-chordate SFZE-containing enhancers exhibited activity in the zebrafish notochord. Furthermore, the SFZE syntax in a non-chordate confers endoderm activity. Our results indicate the ancient association of SFZE with , likely predating the origin of animals. The emergence of notochordal expression could be attributed to co-option of upstream signals acting on the conserved SFZE syntax, which facilitates the origin of the notochord from rudimentary endodermal cells.
format Artículo científico
id pubmed_40712008
institution PubMed
language en
publishDate 2025
publisher Science advances
record_format pubmed
spellingShingle Deep homology of a cis-regulatory syntax and the evolutionary origin of the notochord.
Fan, Tzu-Pei
Lee, Jun-Ru
Lin, Che-Yi
Chen, Yi-Chih
Cutting, Ann E
Cameron, R Andrew
Yu, Jr-Kai
Su, Yi-Hsien
Notochord
T-Box Domain Proteins
Fetal Proteins
Animals
Brachyury Protein
Gene Expression Regulation, Developmental
Zebrafish
Evolution, Molecular
Enhancer Elements, Genetic
Binding Sites
Deep homology of a cis-regulatory syntax and the evolutionary origin of the notochord. Fan, Tzu-Pei Lee, Jun-Ru Lin, Che-Yi Chen, Yi-Chih Cutting, Ann E Cameron, R Andrew Yu, Jr-Kai Su, Yi-Hsien Notochord T-Box Domain Proteins Fetal Proteins Animals Brachyury Protein Gene Expression Regulation, Developmental Zebrafish Evolution, Molecular Enhancer Elements, Genetic Binding Sites Expression of in the notochord is regarded as a chordate novelty and links to the origin of the notochord, yet the evolution of this regulatory control remains unclear. Here, we uncovered a regulatory syntax (named SFZE) consisting of binding sites for four transcription factors in notochord enhancers of chordate genes. SFZE was also identified in potential enhancers in various non-chordate animals and even in , a unicellular relative to animals. These non-chordate SFZE-containing enhancers exhibited activity in the zebrafish notochord. Furthermore, the SFZE syntax in a non-chordate confers endoderm activity. Our results indicate the ancient association of SFZE with , likely predating the origin of animals. The emergence of notochordal expression could be attributed to co-option of upstream signals acting on the conserved SFZE syntax, which facilitates the origin of the notochord from rudimentary endodermal cells.
title Deep homology of a cis-regulatory syntax and the evolutionary origin of the notochord.
topic Notochord
T-Box Domain Proteins
Fetal Proteins
Animals
Brachyury Protein
Gene Expression Regulation, Developmental
Zebrafish
Evolution, Molecular
Enhancer Elements, Genetic
Binding Sites
url https://pubmed.ncbi.nlm.nih.gov/40712008/