sp. nov., a novel member of the family , isolated from a saline lake.
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| Autores principales: | , , , , , , |
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| Formato: | Artículo científico |
| Lenguaje: | en |
| Publicado: |
International journal of systematic and evolutionary microbiology
2025
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| _version_ | 1868266172010463232 |
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| author | Han, Shuai-Bo Hou, Xin-Jun Li, Yu He, Xiao-Long Wang, Rui-Jun Zhu, Xu-Fen Wu, Min |
| author_facet | Han, Shuai-Bo Hou, Xin-Jun Li, Yu He, Xiao-Long Wang, Rui-Jun Zhu, Xu-Fen Wu, Min Han, Shuai-Bo Hou, Xin-Jun Li, Yu He, Xiao-Long Wang, Rui-Jun Zhu, Xu-Fen Wu, Min |
| collection | PubMed - marine biology |
| contents | sp. nov., a novel member of the family , isolated from a saline lake. Han, Shuai-Bo Hou, Xin-Jun Li, Yu He, Xiao-Long Wang, Rui-Jun Zhu, Xu-Fen Wu, Min Phylogeny RNA, Ribosomal, 16S Lakes Bacterial Typing Techniques China Base Composition Fatty Acids DNA, Bacterial Phospholipids Ubiquinone Sequence Analysis, DNA Rhodobacteraceae Nucleic Acid Hybridization A Gram-stain-negative, aerobic bacterium, designated W635, was isolated from saline lake water collected from Lake Ayaqqum in Xinjiang province, China. Cells were catalase and oxidase positive, rod-shaped and non-motile. Growth occurred at 20-37 °C (optimum 28-30 °C), pH 7.0-9.5 (optimum 7.5-8.0) and with 0.5-16.0% NaCl (optimum 2.0-4.0%, w/v). Chemotaxonomic analysis revealed that the sole respiratory quinone was ubiquinone-10. The major fatty acids included C, C cyclo 8, C 7 11-methyl and summed feature 8 (C 7 and/or C 6). The major polar lipids were phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, one uncharacterized phospholipid and four uncharacterized lipids. Sequence similarity analysis of the 16S rRNA gene revealed that strain W635 presented the highest similarity to TFZ (95.47%), followed by members of the genus (95.11-95.25%). Phylogenetic analysis based on multilocus sequence analysis and whole-genome sequencing indicated that strain W635 is most closely related to the genus . The genomic DNA G+C content of the strain W635 was 65.8 mol%. The average amino acid identity values between strain W635 and SSW136, JS7-11, JCM30752, and Z7-4 were 79.02%, 80.04%, 79.77% and 80.05%, respectively. The average nucleotide identity and DNA-DNA hybridization values between strain W635 and these strains were 78.37-79.78% and 19.8-20.80%, respectively. Based on phenotypic, chemotaxonomic, genotypic and genomic characteristics, strain W635 represents a novel species of the genus , for which the name is proposed. The type strain is W635 (=KCTC 62429 =MCCC 1K03491). |
| format | Artículo científico |
| id | pubmed_40719415 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | International journal of systematic and evolutionary microbiology |
| record_format | pubmed |
| spellingShingle | sp. nov., a novel member of the family , isolated from a saline lake. Han, Shuai-Bo Hou, Xin-Jun Li, Yu He, Xiao-Long Wang, Rui-Jun Zhu, Xu-Fen Wu, Min Phylogeny RNA, Ribosomal, 16S Lakes Bacterial Typing Techniques China Base Composition Fatty Acids DNA, Bacterial Phospholipids Ubiquinone Sequence Analysis, DNA Rhodobacteraceae Nucleic Acid Hybridization sp. nov., a novel member of the family , isolated from a saline lake. Han, Shuai-Bo Hou, Xin-Jun Li, Yu He, Xiao-Long Wang, Rui-Jun Zhu, Xu-Fen Wu, Min Phylogeny RNA, Ribosomal, 16S Lakes Bacterial Typing Techniques China Base Composition Fatty Acids DNA, Bacterial Phospholipids Ubiquinone Sequence Analysis, DNA Rhodobacteraceae Nucleic Acid Hybridization A Gram-stain-negative, aerobic bacterium, designated W635, was isolated from saline lake water collected from Lake Ayaqqum in Xinjiang province, China. Cells were catalase and oxidase positive, rod-shaped and non-motile. Growth occurred at 20-37 °C (optimum 28-30 °C), pH 7.0-9.5 (optimum 7.5-8.0) and with 0.5-16.0% NaCl (optimum 2.0-4.0%, w/v). Chemotaxonomic analysis revealed that the sole respiratory quinone was ubiquinone-10. The major fatty acids included C, C cyclo 8, C 7 11-methyl and summed feature 8 (C 7 and/or C 6). The major polar lipids were phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, one uncharacterized phospholipid and four uncharacterized lipids. Sequence similarity analysis of the 16S rRNA gene revealed that strain W635 presented the highest similarity to TFZ (95.47%), followed by members of the genus (95.11-95.25%). Phylogenetic analysis based on multilocus sequence analysis and whole-genome sequencing indicated that strain W635 is most closely related to the genus . The genomic DNA G+C content of the strain W635 was 65.8 mol%. The average amino acid identity values between strain W635 and SSW136, JS7-11, JCM30752, and Z7-4 were 79.02%, 80.04%, 79.77% and 80.05%, respectively. The average nucleotide identity and DNA-DNA hybridization values between strain W635 and these strains were 78.37-79.78% and 19.8-20.80%, respectively. Based on phenotypic, chemotaxonomic, genotypic and genomic characteristics, strain W635 represents a novel species of the genus , for which the name is proposed. The type strain is W635 (=KCTC 62429 =MCCC 1K03491). |
| title | sp. nov., a novel member of the family , isolated from a saline lake. |
| topic | Phylogeny RNA, Ribosomal, 16S Lakes Bacterial Typing Techniques China Base Composition Fatty Acids DNA, Bacterial Phospholipids Ubiquinone Sequence Analysis, DNA Rhodobacteraceae Nucleic Acid Hybridization |
| url | https://pubmed.ncbi.nlm.nih.gov/40719415/ |