The development and plasticity of acid excretion mechanisms in early life stage red drum, Sciaenops ocellatus.
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| Format: | Artículo científico |
| Language: | en |
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Comparative biochemistry and physiology. Part A, Molecular & integrative physiology
2025
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| _version_ | 1868266172006268929 |
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| author | Lonthair, Joshua Esbaugh, Andrew J |
| author_facet | Lonthair, Joshua Esbaugh, Andrew J Lonthair, Joshua Esbaugh, Andrew J |
| collection | PubMed - marine biology |
| contents | The development and plasticity of acid excretion mechanisms in early life stage red drum, Sciaenops ocellatus. Lonthair, Joshua Esbaugh, Andrew J Animals Perciformes Hydrogen-Ion Concentration Seawater Acids Acid-Base Equilibrium Larva Fish Proteins Carbon Dioxide Ocean acidification (OA) has been shown to affect early life stage fishes in a variety of ways, including reduced survival and growth, and increased tissue damage. Yet, there is also substantial interspecies variability in the sensitivity of early life stage fishes to high CO, and it has been theorized that this may relate to the ontogeny of systemic acid-base regulatory pathways; an area that has been surprisingly understudied in obligate marine species. Here, we used an integrative set of approaches to describe the development and plasticity of acid excretion pathways in developing red drum (Sciaenops ocellatus), a marine fish native to the Gulf of Mexico. We observed mRNA expression of relevant transporters and ionocytes immediately post-hatch (36 h post-fertilization, hpf) with relatively stable abundance throughout the pre-metamorphic stages. Consistent with work in adults and seawater acclimated euryhaline larvae, we demonstrate strong co-localization of acid excretion proteins within a single epithelial ionocyte cell-type. Measurements of epithelial Δ[H], an indicator of proton efflux, showed that by 72 hpf larvae had CO-responsive EIPA-sensitive acid excretion, confirming the presence of sodium proton exchanger (NHE)-mediated acid excretion. Elevated mRNA expression of nhe2 and nhe3 was induced following exposure to 5500 and 12,000 μatm CO, which coincided with the absence of further survival effects relative to lower dose CO. Overall, these data confirm that red drum have fully functional epithelial acid excretion pathways in early life, and that plasticity in these pathways may offer survival benefits. |
| format | Artículo científico |
| id | pubmed_40721057 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Comparative biochemistry and physiology. Part A, Molecular & integrative physiology |
| record_format | pubmed |
| spellingShingle | The development and plasticity of acid excretion mechanisms in early life stage red drum, Sciaenops ocellatus. Lonthair, Joshua Esbaugh, Andrew J Animals Perciformes Hydrogen-Ion Concentration Seawater Acids Acid-Base Equilibrium Larva Fish Proteins Carbon Dioxide The development and plasticity of acid excretion mechanisms in early life stage red drum, Sciaenops ocellatus. Lonthair, Joshua Esbaugh, Andrew J Animals Perciformes Hydrogen-Ion Concentration Seawater Acids Acid-Base Equilibrium Larva Fish Proteins Carbon Dioxide Ocean acidification (OA) has been shown to affect early life stage fishes in a variety of ways, including reduced survival and growth, and increased tissue damage. Yet, there is also substantial interspecies variability in the sensitivity of early life stage fishes to high CO, and it has been theorized that this may relate to the ontogeny of systemic acid-base regulatory pathways; an area that has been surprisingly understudied in obligate marine species. Here, we used an integrative set of approaches to describe the development and plasticity of acid excretion pathways in developing red drum (Sciaenops ocellatus), a marine fish native to the Gulf of Mexico. We observed mRNA expression of relevant transporters and ionocytes immediately post-hatch (36 h post-fertilization, hpf) with relatively stable abundance throughout the pre-metamorphic stages. Consistent with work in adults and seawater acclimated euryhaline larvae, we demonstrate strong co-localization of acid excretion proteins within a single epithelial ionocyte cell-type. Measurements of epithelial Δ[H], an indicator of proton efflux, showed that by 72 hpf larvae had CO-responsive EIPA-sensitive acid excretion, confirming the presence of sodium proton exchanger (NHE)-mediated acid excretion. Elevated mRNA expression of nhe2 and nhe3 was induced following exposure to 5500 and 12,000 μatm CO, which coincided with the absence of further survival effects relative to lower dose CO. Overall, these data confirm that red drum have fully functional epithelial acid excretion pathways in early life, and that plasticity in these pathways may offer survival benefits. |
| title | The development and plasticity of acid excretion mechanisms in early life stage red drum, Sciaenops ocellatus. |
| topic | Animals Perciformes Hydrogen-Ion Concentration Seawater Acids Acid-Base Equilibrium Larva Fish Proteins Carbon Dioxide |
| url | https://pubmed.ncbi.nlm.nih.gov/40721057/ |