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| Autores principales: | , , |
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| Formato: | Artículo científico |
| Lenguaje: | en |
| Publicado: |
Animals : an open access journal from MDPI
2025
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| Acceso en línea: | https://pubmed.ncbi.nlm.nih.gov/40723497/ |
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| _version_ | 1868266171998928897 |
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| author | Guo, Song Yang, Linwei Xu, Xiaopeng |
| author_facet | Guo, Song Yang, Linwei Xu, Xiaopeng Guo, Song Yang, Linwei Xu, Xiaopeng |
| collection | PubMed - marine biology |
| contents | Assessing the Tolerance of Spotted Longbarbel Catfish as a Candidate Species for Aquaculture to Ammonia Nitrogen Exposure. Guo, Song Yang, Linwei Xu, Xiaopeng The spotted longbarbel catfish, , a nationally protected Class II species in China, faces increasing threats from habitat degradation. Recently, the spotted longbarbel catfish has gained attention as a promising aquaculture species, not only for its premium flesh quality but also for its potential role in conservation through sustainable captive breeding programs. Ammonia nitrogen (ammonia-N) is a ubiquitous byproduct of intensive farming and serves as the primary environmental stressor confronting aquatic species. Elucidating the ammonia-N tolerance of spotted longbarbel catfish constitutes a critical prerequisite for its successful domestication, which is the aim of this study. We demonstrate that ammonia-N stress significantly decreases the survival rate of spotted longbarbel catfish and induces tissue damage, including gill lamella proliferation, hepatocyte blurring, and renal necrosis. Transcriptomic analysis revealed that ammonia-N stress promotes the expression of genes related to endoplasmic reticulum stress, heat-shock proteins, immune response, and apoptosis, while inhibiting antioxidant-related genes and Wnt-related genes. Enzymatic assays indicate that ammonia-N stress inhibits the activities of multiple antioxidant enzymes, including SOD, CAT, GSH, GSH-Px, and T-AOC. Microbiome analysis showed that ammonia-N stress altered the intestinal microbial community by increasing harmful bacteria (e.g., and ) and suppressing beneficial bacteria (e.g., and ). These findings highlight the comprehensive negative impacts of ammonia-N on the health of the spotted longbarbel catfish and provide a theoretical basis for optimizing aquaculture conditions to support the sustainable protection and domestication of the spotted longbarbel catfish. |
| format | Artículo científico |
| id | pubmed_40723497 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Animals : an open access journal from MDPI |
| record_format | pubmed |
| spellingShingle | Assessing the Tolerance of Spotted Longbarbel Catfish as a Candidate Species for Aquaculture to Ammonia Nitrogen Exposure. Guo, Song Yang, Linwei Xu, Xiaopeng Assessing the Tolerance of Spotted Longbarbel Catfish as a Candidate Species for Aquaculture to Ammonia Nitrogen Exposure. Guo, Song Yang, Linwei Xu, Xiaopeng The spotted longbarbel catfish, , a nationally protected Class II species in China, faces increasing threats from habitat degradation. Recently, the spotted longbarbel catfish has gained attention as a promising aquaculture species, not only for its premium flesh quality but also for its potential role in conservation through sustainable captive breeding programs. Ammonia nitrogen (ammonia-N) is a ubiquitous byproduct of intensive farming and serves as the primary environmental stressor confronting aquatic species. Elucidating the ammonia-N tolerance of spotted longbarbel catfish constitutes a critical prerequisite for its successful domestication, which is the aim of this study. We demonstrate that ammonia-N stress significantly decreases the survival rate of spotted longbarbel catfish and induces tissue damage, including gill lamella proliferation, hepatocyte blurring, and renal necrosis. Transcriptomic analysis revealed that ammonia-N stress promotes the expression of genes related to endoplasmic reticulum stress, heat-shock proteins, immune response, and apoptosis, while inhibiting antioxidant-related genes and Wnt-related genes. Enzymatic assays indicate that ammonia-N stress inhibits the activities of multiple antioxidant enzymes, including SOD, CAT, GSH, GSH-Px, and T-AOC. Microbiome analysis showed that ammonia-N stress altered the intestinal microbial community by increasing harmful bacteria (e.g., and ) and suppressing beneficial bacteria (e.g., and ). These findings highlight the comprehensive negative impacts of ammonia-N on the health of the spotted longbarbel catfish and provide a theoretical basis for optimizing aquaculture conditions to support the sustainable protection and domestication of the spotted longbarbel catfish. |
| title | Assessing the Tolerance of Spotted Longbarbel Catfish as a Candidate Species for Aquaculture to Ammonia Nitrogen Exposure. |
| url | https://pubmed.ncbi.nlm.nih.gov/40723497/ |