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| Main Authors: | , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Animals : an open access journal from MDPI
2025
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| Online Access: | https://pubmed.ncbi.nlm.nih.gov/40723580/ |
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| _version_ | 1868266171997880322 |
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| author | Sohn, Min-Young Kang, Gyoungsik Kim, Kyung-Ho Son, Ha-Jeong Park, Chan-Il |
| author_facet | Sohn, Min-Young Kang, Gyoungsik Kim, Kyung-Ho Son, Ha-Jeong Park, Chan-Il Sohn, Min-Young Kang, Gyoungsik Kim, Kyung-Ho Son, Ha-Jeong Park, Chan-Il |
| collection | PubMed - marine biology |
| contents | Functional Characterization of in the Starry Flounder () and Its Potential as an Immunostimulant. Sohn, Min-Young Kang, Gyoungsik Kim, Kyung-Ho Son, Ha-Jeong Park, Chan-Il () is a central pro-inflammatory cytokine that mediates host immune responses during infection. In this study, we identified and characterized the gene in the starry flounder () through transcriptomic analysis. The deduced protein contained a conserved TNF domain and transmembrane region, and phylogenetic analysis confirmed its homology with other teleost TNFα proteins. Tissue-specific expression profiling revealed high baseline expression in immune-related peripheral organs and a distinct temporal modulation in response to infection. Recombinant TNFα (rTNFα), produced using a cell-free expression system, significantly enhanced phagocytic activity in peripheral and kidney-derived leukocytes in a dose-dependent manner. Peak activity was observed at 150-200 μg/mL, while a decline at higher concentrations suggested a threshold for immune stimulation. Importantly, hemolysis assays confirmed the safety of rTNFα even at the highest tested concentrations. These results demonstrate the immunomodulatory potential of TNFα as a molecular adjuvant in aquaculture vaccines and underscore its potential utility in immune-enhancing strategies for sustainable aquaculture. |
| format | Artículo científico |
| id | pubmed_40723580 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Animals : an open access journal from MDPI |
| record_format | pubmed |
| spellingShingle | Functional Characterization of in the Starry Flounder () and Its Potential as an Immunostimulant. Sohn, Min-Young Kang, Gyoungsik Kim, Kyung-Ho Son, Ha-Jeong Park, Chan-Il Functional Characterization of in the Starry Flounder () and Its Potential as an Immunostimulant. Sohn, Min-Young Kang, Gyoungsik Kim, Kyung-Ho Son, Ha-Jeong Park, Chan-Il () is a central pro-inflammatory cytokine that mediates host immune responses during infection. In this study, we identified and characterized the gene in the starry flounder () through transcriptomic analysis. The deduced protein contained a conserved TNF domain and transmembrane region, and phylogenetic analysis confirmed its homology with other teleost TNFα proteins. Tissue-specific expression profiling revealed high baseline expression in immune-related peripheral organs and a distinct temporal modulation in response to infection. Recombinant TNFα (rTNFα), produced using a cell-free expression system, significantly enhanced phagocytic activity in peripheral and kidney-derived leukocytes in a dose-dependent manner. Peak activity was observed at 150-200 μg/mL, while a decline at higher concentrations suggested a threshold for immune stimulation. Importantly, hemolysis assays confirmed the safety of rTNFα even at the highest tested concentrations. These results demonstrate the immunomodulatory potential of TNFα as a molecular adjuvant in aquaculture vaccines and underscore its potential utility in immune-enhancing strategies for sustainable aquaculture. |
| title | Functional Characterization of in the Starry Flounder () and Its Potential as an Immunostimulant. |
| url | https://pubmed.ncbi.nlm.nih.gov/40723580/ |