Uncoupling growth and division in colonies: consistent cell cycle regulation under confinement.
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PubMed
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| Autori principali: | , , , , , |
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| Natura: | Artículo científico |
| Lingua: | en |
| Pubblicazione: |
ISME communications
2025
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| Accesso online: | |
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| _version_ | 1868266171983200258 |
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| author | Chua, Sing Teng Kotar, Jurij Kühl, Michael Smith, Alison G Vignolini, Silvia Cicuta, Pietro |
| author_facet | Chua, Sing Teng Kotar, Jurij Kühl, Michael Smith, Alison G Vignolini, Silvia Cicuta, Pietro Chua, Sing Teng Kotar, Jurij Kühl, Michael Smith, Alison G Vignolini, Silvia Cicuta, Pietro |
| collection | PubMed - marine biology |
| contents | Uncoupling growth and division in colonies: consistent cell cycle regulation under confinement. Chua, Sing Teng Kotar, Jurij Kühl, Michael Smith, Alison G Vignolini, Silvia Cicuta, Pietro A planar cell microcolony served as a model system to study the impact of inter-cellular crowding and cell-matrix interactions upon the cell cycle. We studied the development over several days of microcolonies, grown from single cells, using a bespoke experimental setup allowing timelapse fluorescence microscopy. Through precise cell segmentation and lineage tracking of a large systematic dataset, characterising individual cell growth and divisions, we uncovered how the external matrix influenced cell cycle and morphology. Experiments also revealed spatial heterogeneity amongst cells within colonies, providing insights into the effects of contact inhibition and micro-gradients of mass transfer. A radial propagation of ring-like pattern, characterised by variations in parent cell size, indicated complex spatio-temporal dynamics in the regulation of the cell cycle within the constrained environment. The mechanisms of commitment and mitotic sizing remained consistent within colonies under this mechanical confinement. These findings contribute to a broader understanding of how matrix immobilisation affects , with implications for alternative culture formats such as biofilms and hydrogel encapsulation-approaches increasingly used in biohybrid applications including biophotovoltaics and bioremediation. |
| format | Artículo científico |
| id | pubmed_40734926 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | ISME communications |
| record_format | pubmed |
| spellingShingle | Uncoupling growth and division in colonies: consistent cell cycle regulation under confinement. Chua, Sing Teng Kotar, Jurij Kühl, Michael Smith, Alison G Vignolini, Silvia Cicuta, Pietro Uncoupling growth and division in colonies: consistent cell cycle regulation under confinement. Chua, Sing Teng Kotar, Jurij Kühl, Michael Smith, Alison G Vignolini, Silvia Cicuta, Pietro A planar cell microcolony served as a model system to study the impact of inter-cellular crowding and cell-matrix interactions upon the cell cycle. We studied the development over several days of microcolonies, grown from single cells, using a bespoke experimental setup allowing timelapse fluorescence microscopy. Through precise cell segmentation and lineage tracking of a large systematic dataset, characterising individual cell growth and divisions, we uncovered how the external matrix influenced cell cycle and morphology. Experiments also revealed spatial heterogeneity amongst cells within colonies, providing insights into the effects of contact inhibition and micro-gradients of mass transfer. A radial propagation of ring-like pattern, characterised by variations in parent cell size, indicated complex spatio-temporal dynamics in the regulation of the cell cycle within the constrained environment. The mechanisms of commitment and mitotic sizing remained consistent within colonies under this mechanical confinement. These findings contribute to a broader understanding of how matrix immobilisation affects , with implications for alternative culture formats such as biofilms and hydrogel encapsulation-approaches increasingly used in biohybrid applications including biophotovoltaics and bioremediation. |
| title | Uncoupling growth and division in colonies: consistent cell cycle regulation under confinement. |
| url | https://pubmed.ncbi.nlm.nih.gov/40734926/ |