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| Autori principali: | , , , , , , , , , , |
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| Natura: | Artículo científico |
| Lingua: | en |
| Pubblicazione: |
Nucleic acids research
2025
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| Soggetti: | |
| Accesso online: | https://pubmed.ncbi.nlm.nih.gov/40744498/ |
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| author | Fuller, Cassandra N Mansoor, Sabrina Jeanne Dit Fouque, Kevin Tose, Lilian Valadares Rodriguez-Casariego, Javier Kosmopoulou, Mariangela Suckau, Detlev de Luna Vitorino, Francisca N Garcia, Benjamin A Eirin-Lopez, Jose M Fernandez-Lima, Francisco |
| author_facet | Fuller, Cassandra N Mansoor, Sabrina Jeanne Dit Fouque, Kevin Tose, Lilian Valadares Rodriguez-Casariego, Javier Kosmopoulou, Mariangela Suckau, Detlev de Luna Vitorino, Francisca N Garcia, Benjamin A Eirin-Lopez, Jose M Fernandez-Lima, Francisco Fuller, Cassandra N Mansoor, Sabrina Jeanne Dit Fouque, Kevin Tose, Lilian Valadares Rodriguez-Casariego, Javier Kosmopoulou, Mariangela Suckau, Detlev de Luna Vitorino, Francisca N Garcia, Benjamin A Eirin-Lopez, Jose M Fernandez-Lima, Francisco |
| collection | PubMed - marine biology |
| contents | Direct histone proteoform profiling of the unannotated, endangered coral Acropora cervicornis. Fuller, Cassandra N Mansoor, Sabrina Jeanne Dit Fouque, Kevin Tose, Lilian Valadares Rodriguez-Casariego, Javier Kosmopoulou, Mariangela Suckau, Detlev de Luna Vitorino, Francisca N Garcia, Benjamin A Eirin-Lopez, Jose M Fernandez-Lima, Francisco Animals Anthozoa Histones Protein Processing, Post-Translational Endangered Species Molecular Sequence Annotation Proteomics Tandem Mass Spectrometry Epigenesis, Genetic Epigenetic modifications directly regulate the patterns of gene expression by altering DNA accessibility and chromatin structure. A knowledge gap is presented by the need to directly measure these modifications, especially for unannotated organisms with unknown primary histone sequences. In the present work, we developed and applied a novel workflow for identifying and annotating histone proteoforms directly from mass spectrometry-based measurements for the endangered Caribbean coral Acropora cervicornis. Combining high-accuracy de novo top-down and bottom-up analysis based on tandem liquid chromatography, trapped ion mobility spectrometry, non-ergodic electron-based fragmentation, and high-resolution mass spectrometry, near complete primary sequence (up to 99%) and over 86 post-translational modification annotations were obtained from pull-down histone fractions. In the absence of reliable genome annotations, H2A, H2B, and H4 histone sequences and the annotation of the post-translational modifications of the stressed A. cervicornis coral allow for a better understanding of chromatin remodeling and new strategies for targeting intervention and restoration of endangered reef corals. |
| format | Artículo científico |
| id | pubmed_40744498 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Nucleic acids research |
| record_format | pubmed |
| spellingShingle | Direct histone proteoform profiling of the unannotated, endangered coral Acropora cervicornis. Fuller, Cassandra N Mansoor, Sabrina Jeanne Dit Fouque, Kevin Tose, Lilian Valadares Rodriguez-Casariego, Javier Kosmopoulou, Mariangela Suckau, Detlev de Luna Vitorino, Francisca N Garcia, Benjamin A Eirin-Lopez, Jose M Fernandez-Lima, Francisco Animals Anthozoa Histones Protein Processing, Post-Translational Endangered Species Molecular Sequence Annotation Proteomics Tandem Mass Spectrometry Epigenesis, Genetic Direct histone proteoform profiling of the unannotated, endangered coral Acropora cervicornis. Fuller, Cassandra N Mansoor, Sabrina Jeanne Dit Fouque, Kevin Tose, Lilian Valadares Rodriguez-Casariego, Javier Kosmopoulou, Mariangela Suckau, Detlev de Luna Vitorino, Francisca N Garcia, Benjamin A Eirin-Lopez, Jose M Fernandez-Lima, Francisco Animals Anthozoa Histones Protein Processing, Post-Translational Endangered Species Molecular Sequence Annotation Proteomics Tandem Mass Spectrometry Epigenesis, Genetic Epigenetic modifications directly regulate the patterns of gene expression by altering DNA accessibility and chromatin structure. A knowledge gap is presented by the need to directly measure these modifications, especially for unannotated organisms with unknown primary histone sequences. In the present work, we developed and applied a novel workflow for identifying and annotating histone proteoforms directly from mass spectrometry-based measurements for the endangered Caribbean coral Acropora cervicornis. Combining high-accuracy de novo top-down and bottom-up analysis based on tandem liquid chromatography, trapped ion mobility spectrometry, non-ergodic electron-based fragmentation, and high-resolution mass spectrometry, near complete primary sequence (up to 99%) and over 86 post-translational modification annotations were obtained from pull-down histone fractions. In the absence of reliable genome annotations, H2A, H2B, and H4 histone sequences and the annotation of the post-translational modifications of the stressed A. cervicornis coral allow for a better understanding of chromatin remodeling and new strategies for targeting intervention and restoration of endangered reef corals. |
| title | Direct histone proteoform profiling of the unannotated, endangered coral Acropora cervicornis. |
| topic | Animals Anthozoa Histones Protein Processing, Post-Translational Endangered Species Molecular Sequence Annotation Proteomics Tandem Mass Spectrometry Epigenesis, Genetic |
| url | https://pubmed.ncbi.nlm.nih.gov/40744498/ |