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| Format: | Artículo científico |
| Sprache: | en |
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Marine environmental research
2025
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| Online-Zugang: | https://pubmed.ncbi.nlm.nih.gov/40752081/ |
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| author | Zhang, Xintang Xu, Fei Hu, Xiaoli Meng, Jie |
| author_facet | Zhang, Xintang Xu, Fei Hu, Xiaoli Meng, Jie Zhang, Xintang Xu, Fei Hu, Xiaoli Meng, Jie |
| collection | PubMed - marine biology |
| contents | Zinc mitigates copper toxicity in Crassostrea gigas by regulating metal homeostasis, oxidative stress, and immune responses. Zhang, Xintang Xu, Fei Hu, Xiaoli Meng, Jie Animals Crassostrea Copper Oxidative Stress Zinc Water Pollutants, Chemical Homeostasis To elucidate the interaction between zinc (Zn) and copper (Cu), this study investigates the mechanisms by which Zn mediates detoxification of Cu toxicity in the Pacific oyster Crassostrea gigas. Physiological indices and transcriptome analyses under control (CTR), Cu, and Zn_Cu treatments reveal that excess Cu induces systemic toxicity in oysters. Cu accumulation in tissues alters metal transporters, downregulating Cu importers and exporters (Zn and Cu transporters) while upregulating chaperones and metallothionein (MT1) for detoxification. Zn supplementation alleviates these effects by enhancing MT1 expression, blocking Cu uptake, and restoring metal homeostasis. Cu stress also increases reactive oxygen species (ROS) and disrupts antioxidant responses, with superoxide dismutase (SOD) overactivation and suppression of catalase1 (CAT1) and glutathione peroxidase (GPX2), leading to potential HO accumulation. Zn supplementation reduces oxidative stress by restoring CAT1 and GPX2 expression, promoting glutathione metabolism, thus maintaining redox balance. Furthermore, Cu suppresses immune signaling by downregulating TLR-MyD88-NF-κB pathway genes and promotes apoptosis through caspase3/7 activation and reduced anti-apoptotic gene expression. Zn partially reverses these effects by reactivating immune genes and reducing caspase levels, though Cu's immune suppression remains dominant. In conclusion, at the tested concentrations (100 μg/L Cu and 50 μg/L Zn) and Zn/Cu ratio, Zn supplementation mitigated Cu-induced oxidative stress and immune suppression in hemocytes, demonstrating its potential for mitigating Cu toxicity in aquaculture. |
| format | Artículo científico |
| id | pubmed_40752081 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Marine environmental research |
| record_format | pubmed |
| spellingShingle | Zinc mitigates copper toxicity in Crassostrea gigas by regulating metal homeostasis, oxidative stress, and immune responses. Zhang, Xintang Xu, Fei Hu, Xiaoli Meng, Jie Animals Crassostrea Copper Oxidative Stress Zinc Water Pollutants, Chemical Homeostasis Zinc mitigates copper toxicity in Crassostrea gigas by regulating metal homeostasis, oxidative stress, and immune responses. Zhang, Xintang Xu, Fei Hu, Xiaoli Meng, Jie Animals Crassostrea Copper Oxidative Stress Zinc Water Pollutants, Chemical Homeostasis To elucidate the interaction between zinc (Zn) and copper (Cu), this study investigates the mechanisms by which Zn mediates detoxification of Cu toxicity in the Pacific oyster Crassostrea gigas. Physiological indices and transcriptome analyses under control (CTR), Cu, and Zn_Cu treatments reveal that excess Cu induces systemic toxicity in oysters. Cu accumulation in tissues alters metal transporters, downregulating Cu importers and exporters (Zn and Cu transporters) while upregulating chaperones and metallothionein (MT1) for detoxification. Zn supplementation alleviates these effects by enhancing MT1 expression, blocking Cu uptake, and restoring metal homeostasis. Cu stress also increases reactive oxygen species (ROS) and disrupts antioxidant responses, with superoxide dismutase (SOD) overactivation and suppression of catalase1 (CAT1) and glutathione peroxidase (GPX2), leading to potential HO accumulation. Zn supplementation reduces oxidative stress by restoring CAT1 and GPX2 expression, promoting glutathione metabolism, thus maintaining redox balance. Furthermore, Cu suppresses immune signaling by downregulating TLR-MyD88-NF-κB pathway genes and promotes apoptosis through caspase3/7 activation and reduced anti-apoptotic gene expression. Zn partially reverses these effects by reactivating immune genes and reducing caspase levels, though Cu's immune suppression remains dominant. In conclusion, at the tested concentrations (100 μg/L Cu and 50 μg/L Zn) and Zn/Cu ratio, Zn supplementation mitigated Cu-induced oxidative stress and immune suppression in hemocytes, demonstrating its potential for mitigating Cu toxicity in aquaculture. |
| title | Zinc mitigates copper toxicity in Crassostrea gigas by regulating metal homeostasis, oxidative stress, and immune responses. |
| topic | Animals Crassostrea Copper Oxidative Stress Zinc Water Pollutants, Chemical Homeostasis |
| url | https://pubmed.ncbi.nlm.nih.gov/40752081/ |