Characterization of Sulfitobacter sediminivivens sp. nov., A Novel Halotolerant, Nitrogen-Assimilating Bacterium from Tidal Flat Sediment.
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| Format: | Artículo científico |
| Language: | en |
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Current microbiology
2025
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| _version_ | 1868266169486540801 |
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| author | Kathiresan, Akila Park, Hee-Yeon Kim, Hyung-Seop Li, Cong Dong, Ke Srinivasan, Sathiyaraj Lee, Sang-Seob |
| author_facet | Kathiresan, Akila Park, Hee-Yeon Kim, Hyung-Seop Li, Cong Dong, Ke Srinivasan, Sathiyaraj Lee, Sang-Seob Kathiresan, Akila Park, Hee-Yeon Kim, Hyung-Seop Li, Cong Dong, Ke Srinivasan, Sathiyaraj Lee, Sang-Seob |
| collection | PubMed - marine biology |
| contents | Characterization of Sulfitobacter sediminivivens sp. nov., A Novel Halotolerant, Nitrogen-Assimilating Bacterium from Tidal Flat Sediment. Kathiresan, Akila Park, Hee-Yeon Kim, Hyung-Seop Li, Cong Dong, Ke Srinivasan, Sathiyaraj Lee, Sang-Seob Geologic Sediments Phylogeny RNA, Ribosomal, 16S Base Composition DNA, Bacterial Fatty Acids Republic of Korea Rhodobacteraceae Bacterial Typing Techniques Nitrogen Nucleic Acid Hybridization Sequence Analysis, DNA Seawater Phospholipids Sodium Chloride A novel halotolerant bacterial strain, designated MF3-043, was isolated from marine sediment collected at the Gunsan tidal flat, South Korea. The strain was characterized as a Gram-negative, aerobic, non-motile, oval-shaped rod bacterium that exhibited catalase and oxidase-positive activities. Colonies were circular, convex, and cream-white in color. The strain was able to grow at temperatures ranging from 4 to 30 °C (optimum, 25 °C), within a pH range of 6.0-9.0 (optimum, pH 7.0), and in the presence of 1-8% (w/v) NaCl (optimum, 2%). Phylogenetic analysis based on 16S rRNA gene sequences revealed the highest sequence similarity to Sulfitobacter mediterraneus KCTC 32188 (97.8%), Sulfitobacter marinus SW-265 (97.7%), Roseobacter fucihabitans B14 (97.6%), Sulfitobacter noctilucae NB-68 (97.4%), and Sulfitobacter indolifex HEL-45 (97.4%), confirming its affiliation with the genus Sulfitobacter. The genomic DNA G + C content was 56.5%. Ubiquinone-10 (Q-10) was the predominant respiratory quinone, and summed feature 8 (Cω7c) was the major fatty acid. Polar lipids included phosphatidylglycerol (PG), phosphatidylcholine (PC), diphosphatidylglycerol (DPG), one aminolipid, one phospholipid, and several unidentified lipids. The genome sequencing and digital DNA-DNA hybridization (dDDH) analyses further supported its distinction from closely related species. Based on a polyphasic taxonomic approach, strain MF3-043 is proposed as a novel species of the genus Sulfitobacter, named Sulfitobacter sediminivivens sp. nov. The type strain is MF3-043 (= KCTC 8706 = TBRC 19386). |
| format | Artículo científico |
| id | pubmed_40764429 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Current microbiology |
| record_format | pubmed |
| spellingShingle | Characterization of Sulfitobacter sediminivivens sp. nov., A Novel Halotolerant, Nitrogen-Assimilating Bacterium from Tidal Flat Sediment. Kathiresan, Akila Park, Hee-Yeon Kim, Hyung-Seop Li, Cong Dong, Ke Srinivasan, Sathiyaraj Lee, Sang-Seob Geologic Sediments Phylogeny RNA, Ribosomal, 16S Base Composition DNA, Bacterial Fatty Acids Republic of Korea Rhodobacteraceae Bacterial Typing Techniques Nitrogen Nucleic Acid Hybridization Sequence Analysis, DNA Seawater Phospholipids Sodium Chloride Characterization of Sulfitobacter sediminivivens sp. nov., A Novel Halotolerant, Nitrogen-Assimilating Bacterium from Tidal Flat Sediment. Kathiresan, Akila Park, Hee-Yeon Kim, Hyung-Seop Li, Cong Dong, Ke Srinivasan, Sathiyaraj Lee, Sang-Seob Geologic Sediments Phylogeny RNA, Ribosomal, 16S Base Composition DNA, Bacterial Fatty Acids Republic of Korea Rhodobacteraceae Bacterial Typing Techniques Nitrogen Nucleic Acid Hybridization Sequence Analysis, DNA Seawater Phospholipids Sodium Chloride A novel halotolerant bacterial strain, designated MF3-043, was isolated from marine sediment collected at the Gunsan tidal flat, South Korea. The strain was characterized as a Gram-negative, aerobic, non-motile, oval-shaped rod bacterium that exhibited catalase and oxidase-positive activities. Colonies were circular, convex, and cream-white in color. The strain was able to grow at temperatures ranging from 4 to 30 °C (optimum, 25 °C), within a pH range of 6.0-9.0 (optimum, pH 7.0), and in the presence of 1-8% (w/v) NaCl (optimum, 2%). Phylogenetic analysis based on 16S rRNA gene sequences revealed the highest sequence similarity to Sulfitobacter mediterraneus KCTC 32188 (97.8%), Sulfitobacter marinus SW-265 (97.7%), Roseobacter fucihabitans B14 (97.6%), Sulfitobacter noctilucae NB-68 (97.4%), and Sulfitobacter indolifex HEL-45 (97.4%), confirming its affiliation with the genus Sulfitobacter. The genomic DNA G + C content was 56.5%. Ubiquinone-10 (Q-10) was the predominant respiratory quinone, and summed feature 8 (Cω7c) was the major fatty acid. Polar lipids included phosphatidylglycerol (PG), phosphatidylcholine (PC), diphosphatidylglycerol (DPG), one aminolipid, one phospholipid, and several unidentified lipids. The genome sequencing and digital DNA-DNA hybridization (dDDH) analyses further supported its distinction from closely related species. Based on a polyphasic taxonomic approach, strain MF3-043 is proposed as a novel species of the genus Sulfitobacter, named Sulfitobacter sediminivivens sp. nov. The type strain is MF3-043 (= KCTC 8706 = TBRC 19386). |
| title | Characterization of Sulfitobacter sediminivivens sp. nov., A Novel Halotolerant, Nitrogen-Assimilating Bacterium from Tidal Flat Sediment. |
| topic | Geologic Sediments Phylogeny RNA, Ribosomal, 16S Base Composition DNA, Bacterial Fatty Acids Republic of Korea Rhodobacteraceae Bacterial Typing Techniques Nitrogen Nucleic Acid Hybridization Sequence Analysis, DNA Seawater Phospholipids Sodium Chloride |
| url | https://pubmed.ncbi.nlm.nih.gov/40764429/ |