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| Autores principales: | , , , , , , , |
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| Formato: | Artículo científico |
| Lenguaje: | en |
| Publicado: |
Virulence
2025
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| Materias: | |
| Acceso en línea: | https://pubmed.ncbi.nlm.nih.gov/40773514/ |
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| _version_ | 1868266167315988480 |
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| author | Kou, Tian-Shun Shang, Yan-Yan Zhang, Qi-Chao Tian, Si-Qi Li, Juan Yang, Li-Na Min, Ling Peng, Bo |
| author_facet | Kou, Tian-Shun Shang, Yan-Yan Zhang, Qi-Chao Tian, Si-Qi Li, Juan Yang, Li-Na Min, Ling Peng, Bo Kou, Tian-Shun Shang, Yan-Yan Zhang, Qi-Chao Tian, Si-Qi Li, Juan Yang, Li-Na Min, Ling Peng, Bo |
| collection | PubMed - marine biology |
| contents | Exogenous proline promotes serum killing of . Kou, Tian-Shun Shang, Yan-Yan Zhang, Qi-Chao Tian, Si-Qi Li, Juan Yang, Li-Na Min, Ling Peng, Bo Klebsiella pneumoniae Animals Klebsiella Infections Mice Proline Humans Blood Bactericidal Activity Complement System Proteins Metabolomics Disease Models, Animal Female Metabolic Networks and Pathways , a common pathogen responsible for bloodstream infections, can evade clearance by the complement-dependent killing in serum, known as serum resistance. However, strategy in managing serum resistance is still lacking. In this study, we employed metabolomics to identify the metabolic features of . We found that the pyruvate/TCA cycle and alanine, aspartate, and glutamate metabolic pathways were significantly downregulated. Proline, identified as a key biomarker, effectively increased the serum sensitivity to multiple clinical isolates and restored the bactericidal activity of complement. The synergistic effect of proline was validated in a murine infection model. Furthermore, we demonstrated that proline activates the pyruvate/TCA cycle, increases proton motive force, and enhances complement proteins binding to bacterial surface, forming membrane attack complex to kill serum-resistant . Our findings provide new insights for the development of metabolism-based approach to manage serum resistance and offer potential targets and strategies for host immunity-based anti-infection therapies. |
| format | Artículo científico |
| id | pubmed_40773514 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Virulence |
| record_format | pubmed |
| spellingShingle | Exogenous proline promotes serum killing of . Kou, Tian-Shun Shang, Yan-Yan Zhang, Qi-Chao Tian, Si-Qi Li, Juan Yang, Li-Na Min, Ling Peng, Bo Klebsiella pneumoniae Animals Klebsiella Infections Mice Proline Humans Blood Bactericidal Activity Complement System Proteins Metabolomics Disease Models, Animal Female Metabolic Networks and Pathways Exogenous proline promotes serum killing of . Kou, Tian-Shun Shang, Yan-Yan Zhang, Qi-Chao Tian, Si-Qi Li, Juan Yang, Li-Na Min, Ling Peng, Bo Klebsiella pneumoniae Animals Klebsiella Infections Mice Proline Humans Blood Bactericidal Activity Complement System Proteins Metabolomics Disease Models, Animal Female Metabolic Networks and Pathways , a common pathogen responsible for bloodstream infections, can evade clearance by the complement-dependent killing in serum, known as serum resistance. However, strategy in managing serum resistance is still lacking. In this study, we employed metabolomics to identify the metabolic features of . We found that the pyruvate/TCA cycle and alanine, aspartate, and glutamate metabolic pathways were significantly downregulated. Proline, identified as a key biomarker, effectively increased the serum sensitivity to multiple clinical isolates and restored the bactericidal activity of complement. The synergistic effect of proline was validated in a murine infection model. Furthermore, we demonstrated that proline activates the pyruvate/TCA cycle, increases proton motive force, and enhances complement proteins binding to bacterial surface, forming membrane attack complex to kill serum-resistant . Our findings provide new insights for the development of metabolism-based approach to manage serum resistance and offer potential targets and strategies for host immunity-based anti-infection therapies. |
| title | Exogenous proline promotes serum killing of . |
| topic | Klebsiella pneumoniae Animals Klebsiella Infections Mice Proline Humans Blood Bactericidal Activity Complement System Proteins Metabolomics Disease Models, Animal Female Metabolic Networks and Pathways |
| url | https://pubmed.ncbi.nlm.nih.gov/40773514/ |