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| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Cell systems
2025
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/40782800/ |
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| _version_ | 1868266167306551298 |
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| author | Traxler, Peter Reichl, Stephan Folkman, Lukas Shaw, Lisa Fife, Victoria Nemc, Amelie Pasajlic, Djurdja Kusienicka, Anna Barreca, Daniele Fortelny, Nikolaus Rendeiro, André F Halbritter, Florian Weninger, Wolfgang Decker, Thomas Farlik, Matthias Bock, Christoph |
| author_facet | Traxler, Peter Reichl, Stephan Folkman, Lukas Shaw, Lisa Fife, Victoria Nemc, Amelie Pasajlic, Djurdja Kusienicka, Anna Barreca, Daniele Fortelny, Nikolaus Rendeiro, André F Halbritter, Florian Weninger, Wolfgang Decker, Thomas Farlik, Matthias Bock, Christoph Traxler, Peter Reichl, Stephan Folkman, Lukas Shaw, Lisa Fife, Victoria Nemc, Amelie Pasajlic, Djurdja Kusienicka, Anna Barreca, Daniele Fortelny, Nikolaus Rendeiro, André F Halbritter, Florian Weninger, Wolfgang Decker, Thomas Farlik, Matthias Bock, Christoph |
| collection | PubMed - marine biology |
| contents | Integrated time-series analysis and high-content CRISPR screening delineate the dynamics of macrophage immune regulation. Traxler, Peter Reichl, Stephan Folkman, Lukas Shaw, Lisa Fife, Victoria Nemc, Amelie Pasajlic, Djurdja Kusienicka, Anna Barreca, Daniele Fortelny, Nikolaus Rendeiro, André F Halbritter, Florian Weninger, Wolfgang Decker, Thomas Farlik, Matthias Bock, Christoph Macrophages Animals Mice Signal Transduction CRISPR-Cas Systems Clustered Regularly Interspaced Short Palindromic Repeats Gene Expression Regulation Macrophages are innate immune cells involved in host defense. Dissecting the regulatory landscape that enables their swift and specific response to pathogens, we performed time-series analysis of gene expression and chromatin accessibility in murine macrophages exposed to various immune stimuli, and we functionally evaluated gene knockouts at scale using a combined CROP-seq and CITE-seq assay. We identified new roles of transcription regulators such as Spi1/PU.1 and JAK-STAT pathway members in immune cell homeostasis and response to pathogens. Macrophage activity was modulated by splicing proteins SFPQ and SF3B1, histone acetyltransferase EP300, cohesin subunit SMC1A, and mediator complex proteins MED8 and MED14. We further observed crosstalk among immune signaling pathways and identified molecular drivers of pathogen-induced dynamics. In summary, this study establishes a time-resolved regulatory map of pathogen response in macrophages, and it describes a broadly applicable method for dissecting immune-regulatory programs through integrative time-series analysis and high-content CRISPR screening. A record of this paper's transparent peer review process is included in the supplemental information. |
| format | Artículo científico |
| id | pubmed_40782800 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Cell systems |
| record_format | pubmed |
| spellingShingle | Integrated time-series analysis and high-content CRISPR screening delineate the dynamics of macrophage immune regulation. Traxler, Peter Reichl, Stephan Folkman, Lukas Shaw, Lisa Fife, Victoria Nemc, Amelie Pasajlic, Djurdja Kusienicka, Anna Barreca, Daniele Fortelny, Nikolaus Rendeiro, André F Halbritter, Florian Weninger, Wolfgang Decker, Thomas Farlik, Matthias Bock, Christoph Macrophages Animals Mice Signal Transduction CRISPR-Cas Systems Clustered Regularly Interspaced Short Palindromic Repeats Gene Expression Regulation Integrated time-series analysis and high-content CRISPR screening delineate the dynamics of macrophage immune regulation. Traxler, Peter Reichl, Stephan Folkman, Lukas Shaw, Lisa Fife, Victoria Nemc, Amelie Pasajlic, Djurdja Kusienicka, Anna Barreca, Daniele Fortelny, Nikolaus Rendeiro, André F Halbritter, Florian Weninger, Wolfgang Decker, Thomas Farlik, Matthias Bock, Christoph Macrophages Animals Mice Signal Transduction CRISPR-Cas Systems Clustered Regularly Interspaced Short Palindromic Repeats Gene Expression Regulation Macrophages are innate immune cells involved in host defense. Dissecting the regulatory landscape that enables their swift and specific response to pathogens, we performed time-series analysis of gene expression and chromatin accessibility in murine macrophages exposed to various immune stimuli, and we functionally evaluated gene knockouts at scale using a combined CROP-seq and CITE-seq assay. We identified new roles of transcription regulators such as Spi1/PU.1 and JAK-STAT pathway members in immune cell homeostasis and response to pathogens. Macrophage activity was modulated by splicing proteins SFPQ and SF3B1, histone acetyltransferase EP300, cohesin subunit SMC1A, and mediator complex proteins MED8 and MED14. We further observed crosstalk among immune signaling pathways and identified molecular drivers of pathogen-induced dynamics. In summary, this study establishes a time-resolved regulatory map of pathogen response in macrophages, and it describes a broadly applicable method for dissecting immune-regulatory programs through integrative time-series analysis and high-content CRISPR screening. A record of this paper's transparent peer review process is included in the supplemental information. |
| title | Integrated time-series analysis and high-content CRISPR screening delineate the dynamics of macrophage immune regulation. |
| topic | Macrophages Animals Mice Signal Transduction CRISPR-Cas Systems Clustered Regularly Interspaced Short Palindromic Repeats Gene Expression Regulation |
| url | https://pubmed.ncbi.nlm.nih.gov/40782800/ |