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| Autori principali: | , , , , |
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| Natura: | Artículo científico |
| Lingua: | en |
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Frontiers in plant science
2025
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| Accesso online: | https://pubmed.ncbi.nlm.nih.gov/40786936/ |
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| _version_ | 1868266167300259841 |
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| author | Li, Shuang Lu, Chang Zang, Xiaonan Duan, Delin Shao, Zhanru |
| author_facet | Li, Shuang Lu, Chang Zang, Xiaonan Duan, Delin Shao, Zhanru Li, Shuang Lu, Chang Zang, Xiaonan Duan, Delin Shao, Zhanru |
| collection | PubMed - marine biology |
| contents | Systems metabolic engineering of for the bioproduction of biliverdin and phycoerythrobilin. Li, Shuang Lu, Chang Zang, Xiaonan Duan, Delin Shao, Zhanru Phycobiliprotein is an important co-pigment in photosynthesis, which is composed of the covalent combination of apoprotein and phycobilin. Biliverdin IXα and phycoerythrobilin are both important substances in the phycobiliprotein biosynthesis pathway. As an economic red seaweed, has a high content of phycoerythrin. Therefore, in this study, we explored new enzyme resources for the heterologous biosynthesis of biliverdin and phycoerythrobilin. Specifically, we identified and isolated the genes encoding NhHO1, NhPebA and NhPebB from , which are integral components of its phycoerythrin biosynthetic pathway. Additionally, from and from phage P-SSM2 were selected for comparative analysis. The results showed that genes from did not encode active enzymes, which might be ascribed to the absence of crucial motifs. However, the transformation of and into could lead to the synthesis of biliverdin and phycoerythrobilin. This is the first report of sequence analysis and enzyme activity verification of phycoerythrin synthesis genes from , providing a foundation for future explorations into its potential genetic resources. The successful production of biliverdin and phycoerythrobilin lay a foundation for the environmentally friendly preparation of phycobiliprotein. |
| format | Artículo científico |
| id | pubmed_40786936 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Frontiers in plant science |
| record_format | pubmed |
| spellingShingle | Systems metabolic engineering of for the bioproduction of biliverdin and phycoerythrobilin. Li, Shuang Lu, Chang Zang, Xiaonan Duan, Delin Shao, Zhanru Systems metabolic engineering of for the bioproduction of biliverdin and phycoerythrobilin. Li, Shuang Lu, Chang Zang, Xiaonan Duan, Delin Shao, Zhanru Phycobiliprotein is an important co-pigment in photosynthesis, which is composed of the covalent combination of apoprotein and phycobilin. Biliverdin IXα and phycoerythrobilin are both important substances in the phycobiliprotein biosynthesis pathway. As an economic red seaweed, has a high content of phycoerythrin. Therefore, in this study, we explored new enzyme resources for the heterologous biosynthesis of biliverdin and phycoerythrobilin. Specifically, we identified and isolated the genes encoding NhHO1, NhPebA and NhPebB from , which are integral components of its phycoerythrin biosynthetic pathway. Additionally, from and from phage P-SSM2 were selected for comparative analysis. The results showed that genes from did not encode active enzymes, which might be ascribed to the absence of crucial motifs. However, the transformation of and into could lead to the synthesis of biliverdin and phycoerythrobilin. This is the first report of sequence analysis and enzyme activity verification of phycoerythrin synthesis genes from , providing a foundation for future explorations into its potential genetic resources. The successful production of biliverdin and phycoerythrobilin lay a foundation for the environmentally friendly preparation of phycobiliprotein. |
| title | Systems metabolic engineering of for the bioproduction of biliverdin and phycoerythrobilin. |
| url | https://pubmed.ncbi.nlm.nih.gov/40786936/ |