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| Format: | Artículo científico |
| Language: | en |
| Published: |
Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]
2025
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/40794376/ |
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| _version_ | 1868266167292919808 |
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| author | Chakroun, Ibtissem Fedhila, Kais Espinosa-Ruiz, Cristóbal Esteban, Maria Angeles Mahdhi, Abdelkarim |
| author_facet | Chakroun, Ibtissem Fedhila, Kais Espinosa-Ruiz, Cristóbal Esteban, Maria Angeles Mahdhi, Abdelkarim Chakroun, Ibtissem Fedhila, Kais Espinosa-Ruiz, Cristóbal Esteban, Maria Angeles Mahdhi, Abdelkarim |
| collection | PubMed - marine biology |
| contents | Impact of Limosilactobacillus reuteri cell-free culture supernatant on stress-resistant Salmonella Typhimurium in seawater: antioxidant, anti-infective, and anti-adhesion effects. Chakroun, Ibtissem Fedhila, Kais Espinosa-Ruiz, Cristóbal Esteban, Maria Angeles Mahdhi, Abdelkarim Salmonella typhimurium Seawater Antioxidants Biofilms Microbial Sensitivity Tests Limosilactobacillus reuteri Bacterial Adhesion Anti-Bacterial Agents Animals Oxidative Stress Salmonellosis linked to fish consumption is a significant public health concern. Salmonella often contaminates fish through polluted water or improper handling, with increasing antibiotic-resistant strains in aquaculture. This study evaluated the antimicrobial, antioxidant, and anti-biofilm activities of the cell-free culture supernatant (CFCS) of Limosilactobacillus reuteri against normal and stressed Salmonella Typhimurium in seawater microcosms over four years. The CFCS exhibited minimum inhibitory concentrations (MIC) ranging from 16.66% to 30% under acidic conditions, whereas stressed strains showed increased resistance, with MIC reaching up to 60% at neutral pH. Antioxidant activity reached 38% (DPPH assay), and CFCS significantly reduced biofilm formation and bacterial adhesion by up to 52% on the fish cell line (SAF-1). Additionally, L. reuteri CFCS modulated the expression of genes related to oxidative stress (cat, sod, gr) and immune response (il1b, il6, il8). These findings highlight the potential of L. reuteri CFCS as a natural antimicrobial and anti-virulence agent in marine aquaculture, helping to address antibiotic resistance issues. |
| format | Artículo científico |
| id | pubmed_40794376 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] |
| record_format | pubmed |
| spellingShingle | Impact of Limosilactobacillus reuteri cell-free culture supernatant on stress-resistant Salmonella Typhimurium in seawater: antioxidant, anti-infective, and anti-adhesion effects. Chakroun, Ibtissem Fedhila, Kais Espinosa-Ruiz, Cristóbal Esteban, Maria Angeles Mahdhi, Abdelkarim Salmonella typhimurium Seawater Antioxidants Biofilms Microbial Sensitivity Tests Limosilactobacillus reuteri Bacterial Adhesion Anti-Bacterial Agents Animals Oxidative Stress Impact of Limosilactobacillus reuteri cell-free culture supernatant on stress-resistant Salmonella Typhimurium in seawater: antioxidant, anti-infective, and anti-adhesion effects. Chakroun, Ibtissem Fedhila, Kais Espinosa-Ruiz, Cristóbal Esteban, Maria Angeles Mahdhi, Abdelkarim Salmonella typhimurium Seawater Antioxidants Biofilms Microbial Sensitivity Tests Limosilactobacillus reuteri Bacterial Adhesion Anti-Bacterial Agents Animals Oxidative Stress Salmonellosis linked to fish consumption is a significant public health concern. Salmonella often contaminates fish through polluted water or improper handling, with increasing antibiotic-resistant strains in aquaculture. This study evaluated the antimicrobial, antioxidant, and anti-biofilm activities of the cell-free culture supernatant (CFCS) of Limosilactobacillus reuteri against normal and stressed Salmonella Typhimurium in seawater microcosms over four years. The CFCS exhibited minimum inhibitory concentrations (MIC) ranging from 16.66% to 30% under acidic conditions, whereas stressed strains showed increased resistance, with MIC reaching up to 60% at neutral pH. Antioxidant activity reached 38% (DPPH assay), and CFCS significantly reduced biofilm formation and bacterial adhesion by up to 52% on the fish cell line (SAF-1). Additionally, L. reuteri CFCS modulated the expression of genes related to oxidative stress (cat, sod, gr) and immune response (il1b, il6, il8). These findings highlight the potential of L. reuteri CFCS as a natural antimicrobial and anti-virulence agent in marine aquaculture, helping to address antibiotic resistance issues. |
| title | Impact of Limosilactobacillus reuteri cell-free culture supernatant on stress-resistant Salmonella Typhimurium in seawater: antioxidant, anti-infective, and anti-adhesion effects. |
| topic | Salmonella typhimurium Seawater Antioxidants Biofilms Microbial Sensitivity Tests Limosilactobacillus reuteri Bacterial Adhesion Anti-Bacterial Agents Animals Oxidative Stress |
| url | https://pubmed.ncbi.nlm.nih.gov/40794376/ |