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| Main Authors: | , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Antonie van Leeuwenhoek
2025
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| Online Access: | https://pubmed.ncbi.nlm.nih.gov/40802082/ |
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| _version_ | 1868266165171650560 |
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| author | Cao, Xingyu Bao, Tianqiang Wang, Jiaxin Yu, Xi Fang, Jiasong |
| author_facet | Cao, Xingyu Bao, Tianqiang Wang, Jiaxin Yu, Xi Fang, Jiasong Cao, Xingyu Bao, Tianqiang Wang, Jiaxin Yu, Xi Fang, Jiasong |
| collection | PubMed - marine biology |
| contents | Maribacter luteocoastalis sp. nov., a novel marine bacterium isolated from the tidal flat sediments of the East China Sea. Cao, Xingyu Bao, Tianqiang Wang, Jiaxin Yu, Xi Fang, Jiasong Phylogeny Geologic Sediments China RNA, Ribosomal, 16S Fatty Acids Base Composition Seawater DNA, Bacterial Bacterial Typing Techniques Phospholipids Nucleic Acid Hybridization Sequence Analysis, DNA A Gram-stain-negative, halophilic, rod-shaped, aerobic bacterial strain, designated as CXY002, was isolated from intertidal seawater of the East China Sea. Strain CXY002 exhibited growth at 4-33 °C (optimum 28 °C), pH 5.5-10 (optimum pH 6-7), and 2-10% (w/v) NaCl (optimum 6%). Based on 16S rRNA gene sequence phylogenetic analysis, strain CXY002 exhibited sequence similarities of 98.20% with Maribacter orientalis DSM 16471, 98.12% with M. spongiicola W15M10, 98.00% with M. stanieri DSM 19891, 98.00% with M. cobaltidurans B1, and 97.72% with M. aquivivus DSM 16478. Phylogenomic reconstruction confirmed its affiliation within the genus Maribacter. Digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between CXY002 and closely related species ranged from 15.20 to 16.30% and 71.90 to 72.46%, respectively, both below the recommended thresholds for species demarcation (70% for dDDH and 95-96% for ANI). The genomic DNA G+C content was determined to be 36.5 mol%. The predominant respiratory quinone was menaquinone-6 (MK-6), while the major fatty acids comprised iso-C (26.7%), 3-OH iso-C (19.9%), and iso-C (16.6%). Polar lipids included phosphatidylethanolamine, two unidentified aminolipids, and four unidentified lipids. Genomic annotation identified complete carotenoid biosynthesis pathways (crt-B, crt-I, crt-Z), supporting which may contribute to oxidative stress resistance via β-carotene and zeaxanthin biosynthesis. Based on polyphasic taxonomic evidence encompassing phenotypic, chemotaxonomic, and genomic characteristics, strain CXY002 represents a novel species within the genus Maribacter, for which the name Maribacter luteocoastalis sp. nov. is proposed. The type strain is CXY002 (= MCCC 1K09937 = KCTC 102438). |
| format | Artículo científico |
| id | pubmed_40802082 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Antonie van Leeuwenhoek |
| record_format | pubmed |
| spellingShingle | Maribacter luteocoastalis sp. nov., a novel marine bacterium isolated from the tidal flat sediments of the East China Sea. Cao, Xingyu Bao, Tianqiang Wang, Jiaxin Yu, Xi Fang, Jiasong Phylogeny Geologic Sediments China RNA, Ribosomal, 16S Fatty Acids Base Composition Seawater DNA, Bacterial Bacterial Typing Techniques Phospholipids Nucleic Acid Hybridization Sequence Analysis, DNA Maribacter luteocoastalis sp. nov., a novel marine bacterium isolated from the tidal flat sediments of the East China Sea. Cao, Xingyu Bao, Tianqiang Wang, Jiaxin Yu, Xi Fang, Jiasong Phylogeny Geologic Sediments China RNA, Ribosomal, 16S Fatty Acids Base Composition Seawater DNA, Bacterial Bacterial Typing Techniques Phospholipids Nucleic Acid Hybridization Sequence Analysis, DNA A Gram-stain-negative, halophilic, rod-shaped, aerobic bacterial strain, designated as CXY002, was isolated from intertidal seawater of the East China Sea. Strain CXY002 exhibited growth at 4-33 °C (optimum 28 °C), pH 5.5-10 (optimum pH 6-7), and 2-10% (w/v) NaCl (optimum 6%). Based on 16S rRNA gene sequence phylogenetic analysis, strain CXY002 exhibited sequence similarities of 98.20% with Maribacter orientalis DSM 16471, 98.12% with M. spongiicola W15M10, 98.00% with M. stanieri DSM 19891, 98.00% with M. cobaltidurans B1, and 97.72% with M. aquivivus DSM 16478. Phylogenomic reconstruction confirmed its affiliation within the genus Maribacter. Digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between CXY002 and closely related species ranged from 15.20 to 16.30% and 71.90 to 72.46%, respectively, both below the recommended thresholds for species demarcation (70% for dDDH and 95-96% for ANI). The genomic DNA G+C content was determined to be 36.5 mol%. The predominant respiratory quinone was menaquinone-6 (MK-6), while the major fatty acids comprised iso-C (26.7%), 3-OH iso-C (19.9%), and iso-C (16.6%). Polar lipids included phosphatidylethanolamine, two unidentified aminolipids, and four unidentified lipids. Genomic annotation identified complete carotenoid biosynthesis pathways (crt-B, crt-I, crt-Z), supporting which may contribute to oxidative stress resistance via β-carotene and zeaxanthin biosynthesis. Based on polyphasic taxonomic evidence encompassing phenotypic, chemotaxonomic, and genomic characteristics, strain CXY002 represents a novel species within the genus Maribacter, for which the name Maribacter luteocoastalis sp. nov. is proposed. The type strain is CXY002 (= MCCC 1K09937 = KCTC 102438). |
| title | Maribacter luteocoastalis sp. nov., a novel marine bacterium isolated from the tidal flat sediments of the East China Sea. |
| topic | Phylogeny Geologic Sediments China RNA, Ribosomal, 16S Fatty Acids Base Composition Seawater DNA, Bacterial Bacterial Typing Techniques Phospholipids Nucleic Acid Hybridization Sequence Analysis, DNA |
| url | https://pubmed.ncbi.nlm.nih.gov/40802082/ |