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| Main Authors: | , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Current microbiology
2025
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/40813730/ |
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| _version_ | 1868266165140193280 |
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| author | Kim, Mirae Lim, Yeonjung Lee, Jayho Nam, Sang-Jip Oh, Dong-Chan Kang, Ilnam Cho, Jang-Cheon |
| author_facet | Kim, Mirae Lim, Yeonjung Lee, Jayho Nam, Sang-Jip Oh, Dong-Chan Kang, Ilnam Cho, Jang-Cheon Kim, Mirae Lim, Yeonjung Lee, Jayho Nam, Sang-Jip Oh, Dong-Chan Kang, Ilnam Cho, Jang-Cheon |
| collection | PubMed - marine biology |
| contents | Nocardiopsis oceanisediminis sp. nov., a Marine-Derived Actinobacterium Isolated from Sediment. Kim, Mirae Lim, Yeonjung Lee, Jayho Nam, Sang-Jip Oh, Dong-Chan Kang, Ilnam Cho, Jang-Cheon Geologic Sediments Phylogeny RNA, Ribosomal, 16S Base Composition Fatty Acids DNA, Bacterial Bacterial Typing Techniques Nucleic Acid Hybridization Genome, Bacterial Actinobacteria Actinomycetales Phospholipids Strain CNT-189, previously isolated from marine sediment and identified as an androsamide producer, was taxonomically characterized in this study. Strain CNT-189 is a Gram-stain-positive, non-motile, pale yellow-pigmented, aerobic actinomycete. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CNT-189 belonged to the genus Nocardiopsis and was most closely related to Nocardiopsis potens DSM 45234 (99.6% similarity). Whole-genome sequencing revealed a DNA G + C content of 74.1% and genome size of 7.12 Mbp. Average nucleotide identity (92.8-95.1%) and digital DNA-DNA hybridization values (45.9-55.4%) between strain CNT-189 and related species were below the species delineation thresholds, supporting its classification as a novel species. The major respiratory quinone was menaquinone-10 (MK-10) and the predominant cellular fatty acids were C ω9c (25.0%), C (13.5%), and anteiso-C (12.7%). Major polar lipids included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylmonomethylethanolamine, phosphatidylinositol mannoside, and monomannosyl diglyceride. Whole-cell hydrolysates contained meso-diaminopimelic acid, glycine, and no diagnostic sugars. Based on phylogenetic and phenotypic evidence, strain CNT-189 is considered to represent a novel species of the genus Nocardiopsis, for which the name Nocardiopsis oceanisediminis sp. nov. is proposed. The type strain is CNT-189 (= KACC 22136 = NBRC 114760). |
| format | Artículo científico |
| id | pubmed_40813730 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Current microbiology |
| record_format | pubmed |
| spellingShingle | Nocardiopsis oceanisediminis sp. nov., a Marine-Derived Actinobacterium Isolated from Sediment. Kim, Mirae Lim, Yeonjung Lee, Jayho Nam, Sang-Jip Oh, Dong-Chan Kang, Ilnam Cho, Jang-Cheon Geologic Sediments Phylogeny RNA, Ribosomal, 16S Base Composition Fatty Acids DNA, Bacterial Bacterial Typing Techniques Nucleic Acid Hybridization Genome, Bacterial Actinobacteria Actinomycetales Phospholipids Nocardiopsis oceanisediminis sp. nov., a Marine-Derived Actinobacterium Isolated from Sediment. Kim, Mirae Lim, Yeonjung Lee, Jayho Nam, Sang-Jip Oh, Dong-Chan Kang, Ilnam Cho, Jang-Cheon Geologic Sediments Phylogeny RNA, Ribosomal, 16S Base Composition Fatty Acids DNA, Bacterial Bacterial Typing Techniques Nucleic Acid Hybridization Genome, Bacterial Actinobacteria Actinomycetales Phospholipids Strain CNT-189, previously isolated from marine sediment and identified as an androsamide producer, was taxonomically characterized in this study. Strain CNT-189 is a Gram-stain-positive, non-motile, pale yellow-pigmented, aerobic actinomycete. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CNT-189 belonged to the genus Nocardiopsis and was most closely related to Nocardiopsis potens DSM 45234 (99.6% similarity). Whole-genome sequencing revealed a DNA G + C content of 74.1% and genome size of 7.12 Mbp. Average nucleotide identity (92.8-95.1%) and digital DNA-DNA hybridization values (45.9-55.4%) between strain CNT-189 and related species were below the species delineation thresholds, supporting its classification as a novel species. The major respiratory quinone was menaquinone-10 (MK-10) and the predominant cellular fatty acids were C ω9c (25.0%), C (13.5%), and anteiso-C (12.7%). Major polar lipids included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylmonomethylethanolamine, phosphatidylinositol mannoside, and monomannosyl diglyceride. Whole-cell hydrolysates contained meso-diaminopimelic acid, glycine, and no diagnostic sugars. Based on phylogenetic and phenotypic evidence, strain CNT-189 is considered to represent a novel species of the genus Nocardiopsis, for which the name Nocardiopsis oceanisediminis sp. nov. is proposed. The type strain is CNT-189 (= KACC 22136 = NBRC 114760). |
| title | Nocardiopsis oceanisediminis sp. nov., a Marine-Derived Actinobacterium Isolated from Sediment. |
| topic | Geologic Sediments Phylogeny RNA, Ribosomal, 16S Base Composition Fatty Acids DNA, Bacterial Bacterial Typing Techniques Nucleic Acid Hybridization Genome, Bacterial Actinobacteria Actinomycetales Phospholipids |
| url | https://pubmed.ncbi.nlm.nih.gov/40813730/ |