Regakine-1 homologue regulates leukocyte migration and activation in large yellow croaker (Larimichthys crocea).
Fuente:
PubMed
Salvato in:
| Autori principali: | , , , , , |
|---|---|
| Natura: | Artículo científico |
| Lingua: | en |
| Pubblicazione: |
Fish & shellfish immunology
2025
|
| Soggetti: | |
| Accesso online: | |
| Tags: |
Aggiungi Tag
Nessun Tag, puoi essere il primo ad aggiungerne!!
|
| _version_ | 1868266165124464641 |
|---|---|
| author | Wang, Meiyan Li, Qiuhua Li, Chenhao Teng, Yan Ao, Jingqun Chen, Xinhua |
| author_facet | Wang, Meiyan Li, Qiuhua Li, Chenhao Teng, Yan Ao, Jingqun Chen, Xinhua Wang, Meiyan Li, Qiuhua Li, Chenhao Teng, Yan Ao, Jingqun Chen, Xinhua |
| collection | PubMed - marine biology |
| contents | Regakine-1 homologue regulates leukocyte migration and activation in large yellow croaker (Larimichthys crocea). Wang, Meiyan Li, Qiuhua Li, Chenhao Teng, Yan Ao, Jingqun Chen, Xinhua Animals Perciformes Fish Proteins Phylogeny Amino Acid Sequence Fish Diseases Leukocytes Sequence Alignment Immunity, Innate Gene Expression Regulation Ciliophora Infections Ciliophora Chemokines, CC Base Sequence Gene Expression Profiling RNA, Messenger Cell Movement Pseudomonas Chemokines are essential components of the vertebrate immune system, playing a crucial role in modulating the migration and functional activation of leukocytes. Among the chemokine superfamily, CC chemokines constitute the largest subgroup in mammals. Although this subfamily exhibits a significant expansion in teleosts, its functional roles remain largely unexplored. In this study, we first identified a Regakine-1 homologue (LcR1), a CC chemokine family member, in large yellow croaker (Larimichthys crocea). The deduced LcR1 protein consists of 142 amino acids, including a 22-residue signal peptide and a 120-residue mature peptide with a conserved CC motif within its chemokine (SCY) domain. Phylogenetic analysis revealed that LcR1 clusters with Regakine-1 homologues from other species. LcR1 mRNA was constitutively expressed in all examined tissues, albeit at varying levels. Following challenge with Cryptocaryon irritans or Pseudomonas plecoglossicida, its transcripts were significantly upregulated in immune-related tissues (head kidney, spleen, gills, and skin), suggesting its involvement in pathogen defense. Recombinant LcR1 (rLcR1) protein not only chemoattracted granulocytes from primary head kidney leukocytes (PKLs) but also enhanced phagocytic activity and respiratory burst of PKLs. Furthermore, rLcR1 significantly upregulated mRNA levels of pro-inflammatory cytokines (il1β, il6, cxcl8_l1, and ifnγ) in PKLs. Collectively, these findings provide the first evidence that fish Regakine-1 acts as an inflammatory chemokine regulating leukocyte migration and activation. |
| format | Artículo científico |
| id | pubmed_40818714 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Fish & shellfish immunology |
| record_format | pubmed |
| spellingShingle | Regakine-1 homologue regulates leukocyte migration and activation in large yellow croaker (Larimichthys crocea). Wang, Meiyan Li, Qiuhua Li, Chenhao Teng, Yan Ao, Jingqun Chen, Xinhua Animals Perciformes Fish Proteins Phylogeny Amino Acid Sequence Fish Diseases Leukocytes Sequence Alignment Immunity, Innate Gene Expression Regulation Ciliophora Infections Ciliophora Chemokines, CC Base Sequence Gene Expression Profiling RNA, Messenger Cell Movement Pseudomonas Regakine-1 homologue regulates leukocyte migration and activation in large yellow croaker (Larimichthys crocea). Wang, Meiyan Li, Qiuhua Li, Chenhao Teng, Yan Ao, Jingqun Chen, Xinhua Animals Perciformes Fish Proteins Phylogeny Amino Acid Sequence Fish Diseases Leukocytes Sequence Alignment Immunity, Innate Gene Expression Regulation Ciliophora Infections Ciliophora Chemokines, CC Base Sequence Gene Expression Profiling RNA, Messenger Cell Movement Pseudomonas Chemokines are essential components of the vertebrate immune system, playing a crucial role in modulating the migration and functional activation of leukocytes. Among the chemokine superfamily, CC chemokines constitute the largest subgroup in mammals. Although this subfamily exhibits a significant expansion in teleosts, its functional roles remain largely unexplored. In this study, we first identified a Regakine-1 homologue (LcR1), a CC chemokine family member, in large yellow croaker (Larimichthys crocea). The deduced LcR1 protein consists of 142 amino acids, including a 22-residue signal peptide and a 120-residue mature peptide with a conserved CC motif within its chemokine (SCY) domain. Phylogenetic analysis revealed that LcR1 clusters with Regakine-1 homologues from other species. LcR1 mRNA was constitutively expressed in all examined tissues, albeit at varying levels. Following challenge with Cryptocaryon irritans or Pseudomonas plecoglossicida, its transcripts were significantly upregulated in immune-related tissues (head kidney, spleen, gills, and skin), suggesting its involvement in pathogen defense. Recombinant LcR1 (rLcR1) protein not only chemoattracted granulocytes from primary head kidney leukocytes (PKLs) but also enhanced phagocytic activity and respiratory burst of PKLs. Furthermore, rLcR1 significantly upregulated mRNA levels of pro-inflammatory cytokines (il1β, il6, cxcl8_l1, and ifnγ) in PKLs. Collectively, these findings provide the first evidence that fish Regakine-1 acts as an inflammatory chemokine regulating leukocyte migration and activation. |
| title | Regakine-1 homologue regulates leukocyte migration and activation in large yellow croaker (Larimichthys crocea). |
| topic | Animals Perciformes Fish Proteins Phylogeny Amino Acid Sequence Fish Diseases Leukocytes Sequence Alignment Immunity, Innate Gene Expression Regulation Ciliophora Infections Ciliophora Chemokines, CC Base Sequence Gene Expression Profiling RNA, Messenger Cell Movement Pseudomonas |
| url | https://pubmed.ncbi.nlm.nih.gov/40818714/ |