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| Format: | Artículo científico |
| Language: | en |
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Current microbiology
2025
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| Online Access: | https://pubmed.ncbi.nlm.nih.gov/40824465/ |
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| _version_ | 1868266165117124608 |
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| author | Kathiresan, Akila Kim, Hyung-Seop Dong, Ke Srinivasan, Sathiyaraj Lee, Sang-Seob |
| author_facet | Kathiresan, Akila Kim, Hyung-Seop Dong, Ke Srinivasan, Sathiyaraj Lee, Sang-Seob Kathiresan, Akila Kim, Hyung-Seop Dong, Ke Srinivasan, Sathiyaraj Lee, Sang-Seob |
| collection | PubMed - marine biology |
| contents | Taxonomic Characterization and Genome Analysis of Polaribacter gochangensis sp. nov., Isolated From a Tidal Clam Farm. Kathiresan, Akila Kim, Hyung-Seop Dong, Ke Srinivasan, Sathiyaraj Lee, Sang-Seob Phylogeny RNA, Ribosomal, 16S Flavobacteriaceae Animals Fatty Acids Genome, Bacterial Bivalvia Base Composition Republic of Korea Geologic Sediments DNA, Bacterial Phospholipids Sequence Analysis, DNA Bacterial Typing Techniques Strain MF5-112 is a Gram-negative, aerobic, non-motile, ovoid, and non-flagellated bacterium with yellow pigmentation that exhibits oxidase-positive and catalase-negative characteristics. It was isolated from tidal mudflat sediment at a clam farm in Gochang, Republic of Korea. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain MF5-112 belongs to the family Flavobacteriaceae. The closest relative was the type strain of Polaribacter huanghezhanensis KCTC 32516 (97.1% 16S rRNA gene sequence similarity), followed by P. pacificus HRA130-1 (96.3%). The major polar lipids were phosphatidylethanolamine, one unidentified phospholipid, two unidentified aminophospholipids, and two unidentified aminolipids. The predominant cellular fatty acids included iso-C, iso-C 3-OH iso-C 3-OH, anteiso-C iso-C G iso-C and Cω6c, with menaquinone-6 identified as the major respiratory quinone. The strain MF5-112 exhibits a dual nitrogen metabolism capability, involving both assimilatory nitrate reduction and partial denitrification, which is essential for nitrogen cycling in marine environments. In 72 h, it reduced ammonia nitrogen (NH₄⁺-N) by 59.5% and nitrate nitrogen (NO₃⁻-N) by 42.0%, with ammonia as the preferred nitrogen source. The genome spans 2.6 Mbp with a G + C content of 31.3%, containing 2,454 genes, including 14 related to nitrogen metabolism pathways. Essential genes such as nit-6, nasBDC, nirK, and nirS facilitate the conversion of nitrate and nitrite to ammonia and further reduce nitrite to nitric oxide (NO) and nitrous oxide (N₂O). These characteristics suggest that strain MF5-112 plays a role in nitrogen cycling within sedimentary ecosystems. Based on its unique genetic and phenotypic properties, strain MF5-112 represents a novel species of the genus Polaribacter, for which the name Polaribacter gochangensis sp. nov. is proposed. The type strain is MF5-112 (= KEMB 23950 = KCTC 102318 = JCM 37723). |
| format | Artículo científico |
| id | pubmed_40824465 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Current microbiology |
| record_format | pubmed |
| spellingShingle | Taxonomic Characterization and Genome Analysis of Polaribacter gochangensis sp. nov., Isolated From a Tidal Clam Farm. Kathiresan, Akila Kim, Hyung-Seop Dong, Ke Srinivasan, Sathiyaraj Lee, Sang-Seob Phylogeny RNA, Ribosomal, 16S Flavobacteriaceae Animals Fatty Acids Genome, Bacterial Bivalvia Base Composition Republic of Korea Geologic Sediments DNA, Bacterial Phospholipids Sequence Analysis, DNA Bacterial Typing Techniques Taxonomic Characterization and Genome Analysis of Polaribacter gochangensis sp. nov., Isolated From a Tidal Clam Farm. Kathiresan, Akila Kim, Hyung-Seop Dong, Ke Srinivasan, Sathiyaraj Lee, Sang-Seob Phylogeny RNA, Ribosomal, 16S Flavobacteriaceae Animals Fatty Acids Genome, Bacterial Bivalvia Base Composition Republic of Korea Geologic Sediments DNA, Bacterial Phospholipids Sequence Analysis, DNA Bacterial Typing Techniques Strain MF5-112 is a Gram-negative, aerobic, non-motile, ovoid, and non-flagellated bacterium with yellow pigmentation that exhibits oxidase-positive and catalase-negative characteristics. It was isolated from tidal mudflat sediment at a clam farm in Gochang, Republic of Korea. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain MF5-112 belongs to the family Flavobacteriaceae. The closest relative was the type strain of Polaribacter huanghezhanensis KCTC 32516 (97.1% 16S rRNA gene sequence similarity), followed by P. pacificus HRA130-1 (96.3%). The major polar lipids were phosphatidylethanolamine, one unidentified phospholipid, two unidentified aminophospholipids, and two unidentified aminolipids. The predominant cellular fatty acids included iso-C, iso-C 3-OH iso-C 3-OH, anteiso-C iso-C G iso-C and Cω6c, with menaquinone-6 identified as the major respiratory quinone. The strain MF5-112 exhibits a dual nitrogen metabolism capability, involving both assimilatory nitrate reduction and partial denitrification, which is essential for nitrogen cycling in marine environments. In 72 h, it reduced ammonia nitrogen (NH₄⁺-N) by 59.5% and nitrate nitrogen (NO₃⁻-N) by 42.0%, with ammonia as the preferred nitrogen source. The genome spans 2.6 Mbp with a G + C content of 31.3%, containing 2,454 genes, including 14 related to nitrogen metabolism pathways. Essential genes such as nit-6, nasBDC, nirK, and nirS facilitate the conversion of nitrate and nitrite to ammonia and further reduce nitrite to nitric oxide (NO) and nitrous oxide (N₂O). These characteristics suggest that strain MF5-112 plays a role in nitrogen cycling within sedimentary ecosystems. Based on its unique genetic and phenotypic properties, strain MF5-112 represents a novel species of the genus Polaribacter, for which the name Polaribacter gochangensis sp. nov. is proposed. The type strain is MF5-112 (= KEMB 23950 = KCTC 102318 = JCM 37723). |
| title | Taxonomic Characterization and Genome Analysis of Polaribacter gochangensis sp. nov., Isolated From a Tidal Clam Farm. |
| topic | Phylogeny RNA, Ribosomal, 16S Flavobacteriaceae Animals Fatty Acids Genome, Bacterial Bivalvia Base Composition Republic of Korea Geologic Sediments DNA, Bacterial Phospholipids Sequence Analysis, DNA Bacterial Typing Techniques |
| url | https://pubmed.ncbi.nlm.nih.gov/40824465/ |