Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Artículo científico |
| Language: | en |
| Published: |
Current microbiology
2025
|
| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/40824465/ |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Table of Contents:
- Taxonomic Characterization and Genome Analysis of Polaribacter gochangensis sp. nov., Isolated From a Tidal Clam Farm. Kathiresan, Akila Kim, Hyung-Seop Dong, Ke Srinivasan, Sathiyaraj Lee, Sang-Seob Phylogeny RNA, Ribosomal, 16S Flavobacteriaceae Animals Fatty Acids Genome, Bacterial Bivalvia Base Composition Republic of Korea Geologic Sediments DNA, Bacterial Phospholipids Sequence Analysis, DNA Bacterial Typing Techniques Strain MF5-112 is a Gram-negative, aerobic, non-motile, ovoid, and non-flagellated bacterium with yellow pigmentation that exhibits oxidase-positive and catalase-negative characteristics. It was isolated from tidal mudflat sediment at a clam farm in Gochang, Republic of Korea. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain MF5-112 belongs to the family Flavobacteriaceae. The closest relative was the type strain of Polaribacter huanghezhanensis KCTC 32516 (97.1% 16S rRNA gene sequence similarity), followed by P. pacificus HRA130-1 (96.3%). The major polar lipids were phosphatidylethanolamine, one unidentified phospholipid, two unidentified aminophospholipids, and two unidentified aminolipids. The predominant cellular fatty acids included iso-C, iso-C 3-OH iso-C 3-OH, anteiso-C iso-C G iso-C and Cω6c, with menaquinone-6 identified as the major respiratory quinone. The strain MF5-112 exhibits a dual nitrogen metabolism capability, involving both assimilatory nitrate reduction and partial denitrification, which is essential for nitrogen cycling in marine environments. In 72 h, it reduced ammonia nitrogen (NH₄⁺-N) by 59.5% and nitrate nitrogen (NO₃⁻-N) by 42.0%, with ammonia as the preferred nitrogen source. The genome spans 2.6 Mbp with a G + C content of 31.3%, containing 2,454 genes, including 14 related to nitrogen metabolism pathways. Essential genes such as nit-6, nasBDC, nirK, and nirS facilitate the conversion of nitrate and nitrite to ammonia and further reduce nitrite to nitric oxide (NO) and nitrous oxide (N₂O). These characteristics suggest that strain MF5-112 plays a role in nitrogen cycling within sedimentary ecosystems. Based on its unique genetic and phenotypic properties, strain MF5-112 represents a novel species of the genus Polaribacter, for which the name Polaribacter gochangensis sp. nov. is proposed. The type strain is MF5-112 (= KEMB 23950 = KCTC 102318 = JCM 37723).