Pangenome biology and evolution in harmful algal-bloom-forming pelagophytes.
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| Main Authors: | , , , , |
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| Format: | Artículo científico |
| Language: | en |
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Current biology : CB
2025
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| _version_ | 1868266162743148544 |
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| author | Sibbald, Shannon J Lawton, Maggie Maclean, Charlotte Roger, Andrew J Archibald, John M |
| author_facet | Sibbald, Shannon J Lawton, Maggie Maclean, Charlotte Roger, Andrew J Archibald, John M Sibbald, Shannon J Lawton, Maggie Maclean, Charlotte Roger, Andrew J Archibald, John M |
| collection | PubMed - marine biology |
| contents | Pangenome biology and evolution in harmful algal-bloom-forming pelagophytes. Sibbald, Shannon J Lawton, Maggie Maclean, Charlotte Roger, Andrew J Archibald, John M Harmful Algal Bloom Phylogeny Gene Transfer, Horizontal Evolution, Molecular Genetic Variation Genome Stramenopiles In prokaryotes, lateral gene transfer (LGT) is a key mechanism leading to intraspecies variability in gene content and the phenomenon of pangenomes. In microbial eukaryotes, however, the extent to which LGT-driven pangenomes exist is unclear. Pelagophytes are ecologically important marine algae that include Aureococcus anophagefferens-a species notorious for causing harmful algal blooms. To investigate genome evolution across Pelagophyceae and within Ac. anophagefferens, we used long-read sequencing to produce high-quality genome assemblies for five strains of Ac. anophagefferens (52-54 megabase pairs [Mbp]), a telomere-to-telomere assembly for Pelagomonas calceolata (32 Mbp), and the first reference genome for Aureoumbra lagunensis (41 Mbp). Using comparative genomics and phylogenetics, we show remarkable strain-level genetic variation in Ac. anophagefferens, with a pangenome (23,356 orthogroups) that is 81.1% core and 18.9% accessory. Although gene content variation within Ac. anophagefferens does not appear to be largely driven by recent prokaryotic LGTs (2.6% of accessory orthogroups), 368 orthogroups were acquired from bacteria in a common ancestor of all analyzed strains and are not found in P. calceolata or Au. lagunensis. A total of 1,077 recent LGTs from prokaryotes and viruses were identified within Pelagophyceae overall, constituting 3.5%-4.0% of the orthogroups in each species. This includes genes likely contributing to the ecological success of pelagophytes globally and in long-lasting harmful blooms. |
| format | Artículo científico |
| id | pubmed_40829589 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Current biology : CB |
| record_format | pubmed |
| spellingShingle | Pangenome biology and evolution in harmful algal-bloom-forming pelagophytes. Sibbald, Shannon J Lawton, Maggie Maclean, Charlotte Roger, Andrew J Archibald, John M Harmful Algal Bloom Phylogeny Gene Transfer, Horizontal Evolution, Molecular Genetic Variation Genome Stramenopiles Pangenome biology and evolution in harmful algal-bloom-forming pelagophytes. Sibbald, Shannon J Lawton, Maggie Maclean, Charlotte Roger, Andrew J Archibald, John M Harmful Algal Bloom Phylogeny Gene Transfer, Horizontal Evolution, Molecular Genetic Variation Genome Stramenopiles In prokaryotes, lateral gene transfer (LGT) is a key mechanism leading to intraspecies variability in gene content and the phenomenon of pangenomes. In microbial eukaryotes, however, the extent to which LGT-driven pangenomes exist is unclear. Pelagophytes are ecologically important marine algae that include Aureococcus anophagefferens-a species notorious for causing harmful algal blooms. To investigate genome evolution across Pelagophyceae and within Ac. anophagefferens, we used long-read sequencing to produce high-quality genome assemblies for five strains of Ac. anophagefferens (52-54 megabase pairs [Mbp]), a telomere-to-telomere assembly for Pelagomonas calceolata (32 Mbp), and the first reference genome for Aureoumbra lagunensis (41 Mbp). Using comparative genomics and phylogenetics, we show remarkable strain-level genetic variation in Ac. anophagefferens, with a pangenome (23,356 orthogroups) that is 81.1% core and 18.9% accessory. Although gene content variation within Ac. anophagefferens does not appear to be largely driven by recent prokaryotic LGTs (2.6% of accessory orthogroups), 368 orthogroups were acquired from bacteria in a common ancestor of all analyzed strains and are not found in P. calceolata or Au. lagunensis. A total of 1,077 recent LGTs from prokaryotes and viruses were identified within Pelagophyceae overall, constituting 3.5%-4.0% of the orthogroups in each species. This includes genes likely contributing to the ecological success of pelagophytes globally and in long-lasting harmful blooms. |
| title | Pangenome biology and evolution in harmful algal-bloom-forming pelagophytes. |
| topic | Harmful Algal Bloom Phylogeny Gene Transfer, Horizontal Evolution, Molecular Genetic Variation Genome Stramenopiles |
| url | https://pubmed.ncbi.nlm.nih.gov/40829589/ |