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| Format: | Artículo científico |
| Sprache: | en |
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ACS applied materials & interfaces
2025
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| Schlagworte: | |
| Online-Zugang: | https://pubmed.ncbi.nlm.nih.gov/40830814/ |
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| _version_ | 1868266162741051392 |
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| author | Zhang, Chen Zhang, Pan-Pan Wei, Cao-Ying Song, Wen-Ye Peng, Hai-Hua Liu, Xin-Xin Zhou, Yong-Can Sun, Yun |
| author_facet | Zhang, Chen Zhang, Pan-Pan Wei, Cao-Ying Song, Wen-Ye Peng, Hai-Hua Liu, Xin-Xin Zhou, Yong-Can Sun, Yun Zhang, Chen Zhang, Pan-Pan Wei, Cao-Ying Song, Wen-Ye Peng, Hai-Hua Liu, Xin-Xin Zhou, Yong-Can Sun, Yun |
| collection | PubMed - marine biology |
| contents | Application of a Rationally Designed Mucosal Nanovaccine Platform in Bacterial Disease Prophylactics Using Fish as a Model Organism. Zhang, Chen Zhang, Pan-Pan Wei, Cao-Ying Song, Wen-Ye Peng, Hai-Hua Liu, Xin-Xin Zhou, Yong-Can Sun, Yun Animals Vibrio Bacterial Vaccines Fish Diseases Nanoparticles Vibrio Infections Fishes Nanovaccines The emergence of antimicrobial resistance necessitates innovative vaccine strategies for bacterial disease prevention in aquaculture. Here, we present a rationally designed mucosal nanovaccine platform targeting , a major pathogen in marine fish. Bioinformatics tools identified 15 T-cell and 16 B-cell epitopes from 17 antigenic proteins, which were consolidated into a multiepitope antigen (MAE). This MAE was fused to the self-assembling ferritin H-subunit (HFn) to form MAE@HFn nanoparticles, further functionalized with PEG2000 (MAE@HFn-PEG) to enhance mucus penetration. Structural characterization confirmed uniform spherical nanoparticles with high stability and negligible cytotoxicity. In marine fish models (grouper), mucosal administration of MAE@HFn-PEG elicited robust humoral and cellular immune responses, including elevated IgM titers, Th1/Th2 cytokine production, and memory T-cell activation. Notably, this nanovaccine achieved an 86% survival rate post- challenge while overcoming traditional limitations of mucosal delivery, such as poor antigen retention. Mechanistic studies revealed efficient transepithelial transport and enhanced antigen presentation, driven by PEG-mediated mucus evasion and HFn's multivalent epitope display. This work establishes a scalable, safe, and economically viable nanoplatform for aquatic disease prophylaxis, with broader implications for mucosal vaccine design in other species. |
| format | Artículo científico |
| id | pubmed_40830814 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | ACS applied materials & interfaces |
| record_format | pubmed |
| spellingShingle | Application of a Rationally Designed Mucosal Nanovaccine Platform in Bacterial Disease Prophylactics Using Fish as a Model Organism. Zhang, Chen Zhang, Pan-Pan Wei, Cao-Ying Song, Wen-Ye Peng, Hai-Hua Liu, Xin-Xin Zhou, Yong-Can Sun, Yun Animals Vibrio Bacterial Vaccines Fish Diseases Nanoparticles Vibrio Infections Fishes Nanovaccines Application of a Rationally Designed Mucosal Nanovaccine Platform in Bacterial Disease Prophylactics Using Fish as a Model Organism. Zhang, Chen Zhang, Pan-Pan Wei, Cao-Ying Song, Wen-Ye Peng, Hai-Hua Liu, Xin-Xin Zhou, Yong-Can Sun, Yun Animals Vibrio Bacterial Vaccines Fish Diseases Nanoparticles Vibrio Infections Fishes Nanovaccines The emergence of antimicrobial resistance necessitates innovative vaccine strategies for bacterial disease prevention in aquaculture. Here, we present a rationally designed mucosal nanovaccine platform targeting , a major pathogen in marine fish. Bioinformatics tools identified 15 T-cell and 16 B-cell epitopes from 17 antigenic proteins, which were consolidated into a multiepitope antigen (MAE). This MAE was fused to the self-assembling ferritin H-subunit (HFn) to form MAE@HFn nanoparticles, further functionalized with PEG2000 (MAE@HFn-PEG) to enhance mucus penetration. Structural characterization confirmed uniform spherical nanoparticles with high stability and negligible cytotoxicity. In marine fish models (grouper), mucosal administration of MAE@HFn-PEG elicited robust humoral and cellular immune responses, including elevated IgM titers, Th1/Th2 cytokine production, and memory T-cell activation. Notably, this nanovaccine achieved an 86% survival rate post- challenge while overcoming traditional limitations of mucosal delivery, such as poor antigen retention. Mechanistic studies revealed efficient transepithelial transport and enhanced antigen presentation, driven by PEG-mediated mucus evasion and HFn's multivalent epitope display. This work establishes a scalable, safe, and economically viable nanoplatform for aquatic disease prophylaxis, with broader implications for mucosal vaccine design in other species. |
| title | Application of a Rationally Designed Mucosal Nanovaccine Platform in Bacterial Disease Prophylactics Using Fish as a Model Organism. |
| topic | Animals Vibrio Bacterial Vaccines Fish Diseases Nanoparticles Vibrio Infections Fishes Nanovaccines |
| url | https://pubmed.ncbi.nlm.nih.gov/40830814/ |