Metagenomic analysis reveals how multiple stressors disrupt virus-host interactions in multi-trophic freshwater mesocosms.
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| Main Authors: | , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
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Nature communications
2025
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| _version_ | 1868266162705399809 |
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| author | Wang, Tao Zhang, Peiyu Anantharaman, Karthik Wang, Huan Zhang, Huan Zhang, Min Xu, Jun |
| author_facet | Wang, Tao Zhang, Peiyu Anantharaman, Karthik Wang, Huan Zhang, Huan Zhang, Min Xu, Jun Wang, Tao Zhang, Peiyu Anantharaman, Karthik Wang, Huan Zhang, Huan Zhang, Min Xu, Jun |
| collection | PubMed - marine biology |
| contents | Metagenomic analysis reveals how multiple stressors disrupt virus-host interactions in multi-trophic freshwater mesocosms. Wang, Tao Zhang, Peiyu Anantharaman, Karthik Wang, Huan Zhang, Huan Zhang, Min Xu, Jun Metagenomics Microbiota Lakes Fresh Water Ecosystem Metagenome Viruses Bacteria Pesticides Host Microbial Interactions Stress, Physiological Climate Change Virus-host interactions are vital to microbiome ecology and evolution, yet their responses to environmental stressors under global change remain poorly understood. We perform a 10-month outdoor mesocosm experiment simulating multi-trophic freshwater shallow lake ecosystems. Using a fully factorial design comprising eight treatments with six replicates each, we assess the individual and combined effects of climate warming, nutrient loading, and pesticide loading on DNA viral communities and their interactions with microbial hosts. Metagenomic sequencing recovers 12,359 viral OTUs and 1628 unique prokaryotic metagenome-assembled genomes. Our analysis shows that combined nutrient and pesticide loading causes significant disruption by synergistically reducing viral alpha diversity while altering beta diversity and predator-prey linkages. Stressors lead to the simplification of virus-bacteria cross-kingdom networks, with nutrient-pesticide combinations exerting the strongest influence, although warming impacts diminish in the presence of pesticides. Stressor-driven changes also affect the abundance and composition of viral auxiliary metabolic genes, leading to complex shifts in virus-mediated metabolic pathways under multiple stress conditions. These findings underscore the importance of understanding the regulatory role of viruses on microbial communities to effectively address the challenges posed by global change. |
| format | Artículo científico |
| id | pubmed_40841555 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Nature communications |
| record_format | pubmed |
| spellingShingle | Metagenomic analysis reveals how multiple stressors disrupt virus-host interactions in multi-trophic freshwater mesocosms. Wang, Tao Zhang, Peiyu Anantharaman, Karthik Wang, Huan Zhang, Huan Zhang, Min Xu, Jun Metagenomics Microbiota Lakes Fresh Water Ecosystem Metagenome Viruses Bacteria Pesticides Host Microbial Interactions Stress, Physiological Climate Change Metagenomic analysis reveals how multiple stressors disrupt virus-host interactions in multi-trophic freshwater mesocosms. Wang, Tao Zhang, Peiyu Anantharaman, Karthik Wang, Huan Zhang, Huan Zhang, Min Xu, Jun Metagenomics Microbiota Lakes Fresh Water Ecosystem Metagenome Viruses Bacteria Pesticides Host Microbial Interactions Stress, Physiological Climate Change Virus-host interactions are vital to microbiome ecology and evolution, yet their responses to environmental stressors under global change remain poorly understood. We perform a 10-month outdoor mesocosm experiment simulating multi-trophic freshwater shallow lake ecosystems. Using a fully factorial design comprising eight treatments with six replicates each, we assess the individual and combined effects of climate warming, nutrient loading, and pesticide loading on DNA viral communities and their interactions with microbial hosts. Metagenomic sequencing recovers 12,359 viral OTUs and 1628 unique prokaryotic metagenome-assembled genomes. Our analysis shows that combined nutrient and pesticide loading causes significant disruption by synergistically reducing viral alpha diversity while altering beta diversity and predator-prey linkages. Stressors lead to the simplification of virus-bacteria cross-kingdom networks, with nutrient-pesticide combinations exerting the strongest influence, although warming impacts diminish in the presence of pesticides. Stressor-driven changes also affect the abundance and composition of viral auxiliary metabolic genes, leading to complex shifts in virus-mediated metabolic pathways under multiple stress conditions. These findings underscore the importance of understanding the regulatory role of viruses on microbial communities to effectively address the challenges posed by global change. |
| title | Metagenomic analysis reveals how multiple stressors disrupt virus-host interactions in multi-trophic freshwater mesocosms. |
| topic | Metagenomics Microbiota Lakes Fresh Water Ecosystem Metagenome Viruses Bacteria Pesticides Host Microbial Interactions Stress, Physiological Climate Change |
| url | https://pubmed.ncbi.nlm.nih.gov/40841555/ |