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Bibliographic Details
Main Authors:	Zhao, Wen-Xiao, Liu, Rui, Wang, Dan-Dan, Gao, Chao, Wang, Peng, Cao, Hai-Yan, Chen, Xiu-Lan, Wang, Yan, Zhang, Yu-Zhong
Format:	Artículo científico
Language:	en
Published:	Journal of agricultural and food chemistry 2025
Subjects:	Substrate Specificity Collagen Collagenases Bacterial Proteins Vibrio Amino Acid Sequence Binding Sites Kinetics Amino Acid Motifs
Online Access:	https://pubmed.ncbi.nlm.nih.gov/40845237/
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Mechanistic Insight into the Cleavage Site Specificity of Collagenase VhaC to the Y-G Bonds in Collagen. Zhao, Wen-Xiao Liu, Rui Wang, Dan-Dan Gao, Chao Wang, Peng Cao, Hai-Yan Chen, Xiu-Lan Wang, Yan Zhang, Yu-Zhong Substrate Specificity Collagen Collagenases Bacterial Proteins Vibrio Amino Acid Sequence Binding Sites Kinetics Amino Acid Motifs M9 collagenases have various biotechnological and medical applications due to their high activity and specificity to collagen. Although the collagenolytic mechanism of M9 collagenases has been studied, the molecular basis for their cleavage site specificity remains unknown. Here, the mechanism of the Y-G bond cleavage site specificity of M9 collagenase VhaC was studied. The double-G motif (G441 and G442) and Y549 of VhaC define a narrow S1' substrate binding pocket responsible for P1' substrate binding, among which Y549 functions as the structural determinant for the specific recognition of P1' glycine, because its bulky side chain restricts the accommodation of residues with a side chain. The S1' tyrosine corresponding to Y549 in VhaC is strictly conserved among M9 collagenases, suggesting its significance in the cleavage site specificity. This study advances the applications of M9 collagenases in disease treatment and collagen oligopeptide production.

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// Anecdotal testing Today and Yesterday seems to indicate destroy() // is called by the garbage collector and everything is good. // Something to keep in mind though. return 0; } /** * Write function that is called when session data is to be saved. * * @param string $sessId The current session ID * @param string $data The session data to write * * @return bool */ public function write($sessId, $data): bool { if ($this->writesDisabled) { return true; } return $this->saveSession($sessId, $data); } /** * A function that is called internally when session data is to be saved. * * @param string $sessId The current session ID * @param string $data The session data to write * * @return bool */ abstract protected function saveSession($sessId, $data): bool; }

* * @return void */ public function writeClose() { // The assumption is that we're using PHP's ext/session. // session_write_close() will actually overwrite $_SESSION with an // empty array on completion -- which leads to a mismatch between what // is in the storage object and $_SESSION. To get around this, we // temporarily reset $_SESSION to an array, and then re-link it to // the storage object. // // Additionally, while you _can_ write to $_SESSION following a // session_write_close() operation, no changes made to it will be // flushed to the session handler. As such, we now mark the storage // object isImmutable. $storage = $this->getStorage(); if (! $storage->isImmutable()) { $_SESSION = $storage->toArray(true); session_write_close(); $storage->fromArray($_SESSION); $storage->markImmutable(); } } /** * Attempt to set the session name * * If the session has already been started, or if the name provided fails * validation, an exception will be raised. * * @param string $name * @return SessionManager * @throws Exception\InvalidArgumentException */ public function setName($name) { if ($this->sessionExists()) { throw new Exception\InvalidArgumentException( 'Cannot set session name after a session has already started'

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