Antibiofilm from phyllosphere improves survival and gut health of juvenile pearl gentian hybrid grouper challenged with Vibrio harveyi.
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| Natura: | Artículo científico |
| Lingua: | en |
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Scientific reports
2025
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| _version_ | 1868266160107028481 |
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| author | Julyantoro, Pande Gde Sasmita Waturangi, Diana Elizabeth Papuangan, Nurmaya Permana, I Gusti Ngurah Mahardika, Ketut |
| author_facet | Julyantoro, Pande Gde Sasmita Waturangi, Diana Elizabeth Papuangan, Nurmaya Permana, I Gusti Ngurah Mahardika, Ketut Julyantoro, Pande Gde Sasmita Waturangi, Diana Elizabeth Papuangan, Nurmaya Permana, I Gusti Ngurah Mahardika, Ketut |
| collection | PubMed - marine biology |
| contents | Antibiofilm from phyllosphere improves survival and gut health of juvenile pearl gentian hybrid grouper challenged with Vibrio harveyi. Julyantoro, Pande Gde Sasmita Waturangi, Diana Elizabeth Papuangan, Nurmaya Permana, I Gusti Ngurah Mahardika, Ketut Animals Vibrio Biofilms Vibrio Infections Fish Diseases Aquaculture Bass Anti-Bacterial Agents Animal Feed The widespread use of antibiotics in aquaculture has become increasingly ineffective due to the emergence of antibiotic-resistant bacteria, residual contamination, the disruption of aquatic microbiota, and international trade restrictions on antibiotic-laden products. As a sustainable alternative, this study investigated the effects of antibiofilm compounds derived from phyllosphere bacteria on the culture performance of juvenile pearl gentian hybrid grouper (Epinephelus fuscoguttatus × E. lanceolatus) challenged with Vibrio harveyi. Juveniles (1.973 ± 0.177 g; 3.421 ± 0.177 cm) were acclimated and distributed into 12 fiber tanks (300 L) at a density of 200 fish per tank. Fish were fed commercial feed supplemented with antibiofilm at concentrations of 0.01, 0.05, and 0.1 mL/kg for four weeks, alongside an unsupplemented control group. Culture was maintained under a flow-through system (10 mL/s). Evaluated parameters included growth, survival, deformity rate, skin pigmentation, water quality, total bacterial and Vibrio abundance, and intestinal morphology, which was assessed via scanning electron microscopy (SEM) and histological analysis (goblet cell count, mucosal fold height, and muscularis thickness). A challenge test was conducted by intraperitoneal injection of V. harveyi (2.3 × 10⁸ CFU/mL; 0.1 mL/fish), followed by a seven-day observation period. The results indicate that while growth was not significantly affected (P > 0.05), fish receiving 0.01 mL/kg antibiofilm exhibited significantly higher survival (P |
| format | Artículo científico |
| id | pubmed_40855171 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Scientific reports |
| record_format | pubmed |
| spellingShingle | Antibiofilm from phyllosphere improves survival and gut health of juvenile pearl gentian hybrid grouper challenged with Vibrio harveyi. Julyantoro, Pande Gde Sasmita Waturangi, Diana Elizabeth Papuangan, Nurmaya Permana, I Gusti Ngurah Mahardika, Ketut Animals Vibrio Biofilms Vibrio Infections Fish Diseases Aquaculture Bass Anti-Bacterial Agents Animal Feed Antibiofilm from phyllosphere improves survival and gut health of juvenile pearl gentian hybrid grouper challenged with Vibrio harveyi. Julyantoro, Pande Gde Sasmita Waturangi, Diana Elizabeth Papuangan, Nurmaya Permana, I Gusti Ngurah Mahardika, Ketut Animals Vibrio Biofilms Vibrio Infections Fish Diseases Aquaculture Bass Anti-Bacterial Agents Animal Feed The widespread use of antibiotics in aquaculture has become increasingly ineffective due to the emergence of antibiotic-resistant bacteria, residual contamination, the disruption of aquatic microbiota, and international trade restrictions on antibiotic-laden products. As a sustainable alternative, this study investigated the effects of antibiofilm compounds derived from phyllosphere bacteria on the culture performance of juvenile pearl gentian hybrid grouper (Epinephelus fuscoguttatus × E. lanceolatus) challenged with Vibrio harveyi. Juveniles (1.973 ± 0.177 g; 3.421 ± 0.177 cm) were acclimated and distributed into 12 fiber tanks (300 L) at a density of 200 fish per tank. Fish were fed commercial feed supplemented with antibiofilm at concentrations of 0.01, 0.05, and 0.1 mL/kg for four weeks, alongside an unsupplemented control group. Culture was maintained under a flow-through system (10 mL/s). Evaluated parameters included growth, survival, deformity rate, skin pigmentation, water quality, total bacterial and Vibrio abundance, and intestinal morphology, which was assessed via scanning electron microscopy (SEM) and histological analysis (goblet cell count, mucosal fold height, and muscularis thickness). A challenge test was conducted by intraperitoneal injection of V. harveyi (2.3 × 10⁸ CFU/mL; 0.1 mL/fish), followed by a seven-day observation period. The results indicate that while growth was not significantly affected (P > 0.05), fish receiving 0.01 mL/kg antibiofilm exhibited significantly higher survival (P |
| title | Antibiofilm from phyllosphere improves survival and gut health of juvenile pearl gentian hybrid grouper challenged with Vibrio harveyi. |
| topic | Animals Vibrio Biofilms Vibrio Infections Fish Diseases Aquaculture Bass Anti-Bacterial Agents Animal Feed |
| url | https://pubmed.ncbi.nlm.nih.gov/40855171/ |