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| Main Authors: | , , , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Marine drugs
2025
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/40863611/ |
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| _version_ | 1868266160098639873 |
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| author | Lu, Jing Shi, Pujing Cao, Yutian Shi, Bingxin Shen, Huilin Zhao, Shuai Gao, Yuchen Chi, Huibing Wang, Lei Shi, Yawei |
| author_facet | Lu, Jing Shi, Pujing Cao, Yutian Shi, Bingxin Shen, Huilin Zhao, Shuai Gao, Yuchen Chi, Huibing Wang, Lei Shi, Yawei Lu, Jing Shi, Pujing Cao, Yutian Shi, Bingxin Shen, Huilin Zhao, Shuai Gao, Yuchen Chi, Huibing Wang, Lei Shi, Yawei |
| collection | PubMed - marine biology |
| contents | Isolation and Purification of Novel Antioxidant Peptides from Mussel () Prepared by Marine Z-1 Protease. Lu, Jing Shi, Pujing Cao, Yutian Shi, Bingxin Shen, Huilin Zhao, Shuai Gao, Yuchen Chi, Huibing Wang, Lei Shi, Yawei Animals Antioxidants Bacillus Peptides Mytilus edulis Molecular Docking Simulation Peptide Hydrolases Tandem Mass Spectrometry Mussels are nutrient-rich but perishable, resulting in substantial resource loss. A protease-producing strain ( Z-1, ) isolated from marine sludge was used to hydrolyze mussels, producing Y-1, a hydrolysate with antioxidant activity. In this study, ultrafiltration, gel chromatography, and LC-MS/MS were employed to isolate and identify bioactive peptides from the hydrolysate. The results revealed that the hydrolysate exhibited antioxidant activity, pancreatic cholesterol esterase inhibitory activity, pancreatic lipase inhibitory activity, and α-glucosidase inhibitory activity. Molecular docking using AutoDock Tools 1.5.6 was performed to analyze the interactions of peptides with CD38 and Keap1, leading to the identification of five potentially bioactive peptides: VPPFY, IMLFP, LPFLF, FLPF, and FPRIM. These peptides formed hydrogen bonds and hydrophobic interactions with CD38 and Keap1, demonstrating strong DPPH radical scavenging and superoxide anion radical scavenging capacities. This study highlights the multifunctional bioactive potential of these peptides, offering insights into their therapeutic applications. The findings provide a novel approach for the effective utilization of mussel resources and highlight their potential application value in the development of functional foods. |
| format | Artículo científico |
| id | pubmed_40863611 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Marine drugs |
| record_format | pubmed |
| spellingShingle | Isolation and Purification of Novel Antioxidant Peptides from Mussel () Prepared by Marine Z-1 Protease. Lu, Jing Shi, Pujing Cao, Yutian Shi, Bingxin Shen, Huilin Zhao, Shuai Gao, Yuchen Chi, Huibing Wang, Lei Shi, Yawei Animals Antioxidants Bacillus Peptides Mytilus edulis Molecular Docking Simulation Peptide Hydrolases Tandem Mass Spectrometry Isolation and Purification of Novel Antioxidant Peptides from Mussel () Prepared by Marine Z-1 Protease. Lu, Jing Shi, Pujing Cao, Yutian Shi, Bingxin Shen, Huilin Zhao, Shuai Gao, Yuchen Chi, Huibing Wang, Lei Shi, Yawei Animals Antioxidants Bacillus Peptides Mytilus edulis Molecular Docking Simulation Peptide Hydrolases Tandem Mass Spectrometry Mussels are nutrient-rich but perishable, resulting in substantial resource loss. A protease-producing strain ( Z-1, ) isolated from marine sludge was used to hydrolyze mussels, producing Y-1, a hydrolysate with antioxidant activity. In this study, ultrafiltration, gel chromatography, and LC-MS/MS were employed to isolate and identify bioactive peptides from the hydrolysate. The results revealed that the hydrolysate exhibited antioxidant activity, pancreatic cholesterol esterase inhibitory activity, pancreatic lipase inhibitory activity, and α-glucosidase inhibitory activity. Molecular docking using AutoDock Tools 1.5.6 was performed to analyze the interactions of peptides with CD38 and Keap1, leading to the identification of five potentially bioactive peptides: VPPFY, IMLFP, LPFLF, FLPF, and FPRIM. These peptides formed hydrogen bonds and hydrophobic interactions with CD38 and Keap1, demonstrating strong DPPH radical scavenging and superoxide anion radical scavenging capacities. This study highlights the multifunctional bioactive potential of these peptides, offering insights into their therapeutic applications. The findings provide a novel approach for the effective utilization of mussel resources and highlight their potential application value in the development of functional foods. |
| title | Isolation and Purification of Novel Antioxidant Peptides from Mussel () Prepared by Marine Z-1 Protease. |
| topic | Animals Antioxidants Bacillus Peptides Mytilus edulis Molecular Docking Simulation Peptide Hydrolases Tandem Mass Spectrometry |
| url | https://pubmed.ncbi.nlm.nih.gov/40863611/ |