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Main Authors:	Chang, Xinyao, Han, Wentao, Chen, Xiaomei, Tang, Caiyin, Wang, Danyang, Huang, Heng, Li, Yuli, Chen, Kai, Hu, Jingjie, Bao, Zhenmin, Chen, Hong, Wang, Shi
Format:	Artículo científico
Language:	en
Published:	Scientific data 2025
Subjects:	Animals Anthozoa Coral Reefs Genome Molecular Sequence Annotation
Online Access:	https://pubmed.ncbi.nlm.nih.gov/40877298/
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Re-annotation improved large-scale assembly of the reef-building coral Acropora intermedia. Chang, Xinyao Han, Wentao Chen, Xiaomei Tang, Caiyin Wang, Danyang Huang, Heng Li, Yuli Chen, Kai Hu, Jingjie Bao, Zhenmin Chen, Hong Wang, Shi Animals Anthozoa Coral Reefs Genome Molecular Sequence Annotation Acropora corals, primary reef-builders providing habitat for numerous marine species, now face novel survival pressures due to environmental changes. Acropora intermedia (Brook 1891), a significant contributor to the vibrant ecosystems of coral reefs in the Indo-Pacific Ocean, also exhibits enhanced resistance to both thermal and acid stress. To advance future studies, we report an improved high-quality genome assembly for A. intermedia obtained through PacBio Hi-Fi long-read sequencing, with a total size of 496.8 Mb. Compared to the previous version, our genome assembly shows substantial improvements in contiguity, with the Contig N50 increasing from 40.3 Kb to 2.9 Mb, and the number of contigs decreasing from 20,998 to 633. Specifically, our genome exhibits no undetectable ambiguous bases (N's) per 100 Kbp, which is remarkably lower than the previous version (5,276.11 per 100 Kbp). The percentage of assembly completeness evaluation based on Benchmarking Universal Single-Copy Orthologs (BUSCO) has increased from 90.6% to 92.6%. We predict a total of 26,852 protein-coding genes, with a BUSCO completeness of 95.7%, marking a 2.7% increase from the previous assembly. Our re-annotation and improved genome assembly of A. intermedia provide a valuable resource for further studies on coral adaptation mechanisms under climate change, and will facilitate comparative and evolutionary research of Acropora.

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// Anecdotal testing Today and Yesterday seems to indicate destroy() // is called by the garbage collector and everything is good. // Something to keep in mind though. return 0; } /** * Write function that is called when session data is to be saved. * * @param string $sessId The current session ID * @param string $data The session data to write * * @return bool */ public function write($sessId, $data): bool { if ($this->writesDisabled) { return true; } return $this->saveSession($sessId, $data); } /** * A function that is called internally when session data is to be saved. * * @param string $sessId The current session ID * @param string $data The session data to write * * @return bool */ abstract protected function saveSession($sessId, $data): bool; }

* * @return void */ public function writeClose() { // The assumption is that we're using PHP's ext/session. // session_write_close() will actually overwrite $_SESSION with an // empty array on completion -- which leads to a mismatch between what // is in the storage object and $_SESSION. To get around this, we // temporarily reset $_SESSION to an array, and then re-link it to // the storage object. // // Additionally, while you _can_ write to $_SESSION following a // session_write_close() operation, no changes made to it will be // flushed to the session handler. As such, we now mark the storage // object isImmutable. $storage = $this->getStorage(); if (! $storage->isImmutable()) { $_SESSION = $storage->toArray(true); session_write_close(); $storage->fromArray($_SESSION); $storage->markImmutable(); } } /** * Attempt to set the session name * * If the session has already been started, or if the name provided fails * validation, an exception will be raised. * * @param string $name * @return SessionManager * @throws Exception\InvalidArgumentException */ public function setName($name) { if ($this->sessionExists()) { throw new Exception\InvalidArgumentException( 'Cannot set session name after a session has already started'

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