Mobilome-mediated transcriptional activation of biosynthetic gene clusters and its impact on strain competitiveness in food fermentation microbiomes.
Fuente:
PubMed
Guardado en:
| Autores principales: | , , , , , , |
|---|---|
| Formato: | Artículo científico |
| Lenguaje: | en |
| Publicado: |
Microbiome
2025
|
| Materias: | |
| Acceso en línea: | |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| _version_ | 1868266160064036865 |
|---|---|
| author | Xu, Lei Jiao, Jian-Yu Ling, Chen Du, Ru-Bing Wu, Qun Xu, Yan Li, Wen-Jun |
| author_facet | Xu, Lei Jiao, Jian-Yu Ling, Chen Du, Ru-Bing Wu, Qun Xu, Yan Li, Wen-Jun Xu, Lei Jiao, Jian-Yu Ling, Chen Du, Ru-Bing Wu, Qun Xu, Yan Li, Wen-Jun |
| collection | PubMed - marine biology |
| contents | Mobilome-mediated transcriptional activation of biosynthetic gene clusters and its impact on strain competitiveness in food fermentation microbiomes. Xu, Lei Jiao, Jian-Yu Ling, Chen Du, Ru-Bing Wu, Qun Xu, Yan Li, Wen-Jun Fermentation Microbiota Multigene Family Metagenome Bacteria Transcriptional Activation Gene Transfer, Horizontal Metagenomics Interspersed Repetitive Sequences Food Microbiology Bacillota Fermented Foods Microbial Interactions Microbial interactions are critical for maintaining the stability of food fermentation microbiomes, and mobile genetic elements (MGEs) significantly influence these interactions by horizontal gene transfer events. Although MGEs are known to facilitate horizontal gene transfer, their distribution among microorganisms and specific effects on microbial interactions remain poorly understood. We analyzed 590 metagenomic and 42 metatranscriptomic samples from food fermentations, recovering 1133 metagenome-assembled genomes (MAGs). Our analysis revealed that MGEs were widely distributed in food fermentation microbiomes, with higher occurrence rates in Firmicutes (Bacillota: 0.71 ~ 11.85%) and Proteobacteria (Pseudomonadota: 0.47 ~ 11.05%). MGEs tended to be located adjacent to functional genes, particularly biosynthetic gene clusters (BGCs), with co-occurrence rates ranging from 9.41 to 23.99%. Furthermore, the transcriptional activity of BGCs was significantly correlated with the number of MGEs that were co-located with BGCs, which might enhance the competitiveness of strains. Variability in the diversity of MGEs that were co-located with BGCs was also evident at the strain level. Using Lactiplantibacillus plantarum as a case, we revealed that the strain-level differences in MGEs that were co-located with BGCs are positively correlated with the transcription of BGCs and competitiveness of strains within the species. This study highlighted the role of MGEs in enhancing transcription of BGCs and facilitating strain competitiveness, providing new insights into how MGEs enhance the adaptability of microbial communities. Video Abstract. |
| format | Artículo científico |
| id | pubmed_40877975 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Microbiome |
| record_format | pubmed |
| spellingShingle | Mobilome-mediated transcriptional activation of biosynthetic gene clusters and its impact on strain competitiveness in food fermentation microbiomes. Xu, Lei Jiao, Jian-Yu Ling, Chen Du, Ru-Bing Wu, Qun Xu, Yan Li, Wen-Jun Fermentation Microbiota Multigene Family Metagenome Bacteria Transcriptional Activation Gene Transfer, Horizontal Metagenomics Interspersed Repetitive Sequences Food Microbiology Bacillota Fermented Foods Microbial Interactions Mobilome-mediated transcriptional activation of biosynthetic gene clusters and its impact on strain competitiveness in food fermentation microbiomes. Xu, Lei Jiao, Jian-Yu Ling, Chen Du, Ru-Bing Wu, Qun Xu, Yan Li, Wen-Jun Fermentation Microbiota Multigene Family Metagenome Bacteria Transcriptional Activation Gene Transfer, Horizontal Metagenomics Interspersed Repetitive Sequences Food Microbiology Bacillota Fermented Foods Microbial Interactions Microbial interactions are critical for maintaining the stability of food fermentation microbiomes, and mobile genetic elements (MGEs) significantly influence these interactions by horizontal gene transfer events. Although MGEs are known to facilitate horizontal gene transfer, their distribution among microorganisms and specific effects on microbial interactions remain poorly understood. We analyzed 590 metagenomic and 42 metatranscriptomic samples from food fermentations, recovering 1133 metagenome-assembled genomes (MAGs). Our analysis revealed that MGEs were widely distributed in food fermentation microbiomes, with higher occurrence rates in Firmicutes (Bacillota: 0.71 ~ 11.85%) and Proteobacteria (Pseudomonadota: 0.47 ~ 11.05%). MGEs tended to be located adjacent to functional genes, particularly biosynthetic gene clusters (BGCs), with co-occurrence rates ranging from 9.41 to 23.99%. Furthermore, the transcriptional activity of BGCs was significantly correlated with the number of MGEs that were co-located with BGCs, which might enhance the competitiveness of strains. Variability in the diversity of MGEs that were co-located with BGCs was also evident at the strain level. Using Lactiplantibacillus plantarum as a case, we revealed that the strain-level differences in MGEs that were co-located with BGCs are positively correlated with the transcription of BGCs and competitiveness of strains within the species. This study highlighted the role of MGEs in enhancing transcription of BGCs and facilitating strain competitiveness, providing new insights into how MGEs enhance the adaptability of microbial communities. Video Abstract. |
| title | Mobilome-mediated transcriptional activation of biosynthetic gene clusters and its impact on strain competitiveness in food fermentation microbiomes. |
| topic | Fermentation Microbiota Multigene Family Metagenome Bacteria Transcriptional Activation Gene Transfer, Horizontal Metagenomics Interspersed Repetitive Sequences Food Microbiology Bacillota Fermented Foods Microbial Interactions |
| url | https://pubmed.ncbi.nlm.nih.gov/40877975/ |