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| Format: | Artículo científico |
| Sprache: | en |
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Open biology
2025
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| Online-Zugang: | https://pubmed.ncbi.nlm.nih.gov/40898956/ |
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| author | Bainour, Khalida Zulkifli, Nabilah Sam, Ka-Kei Navarro, Juan C Castro, Luis Filipe C Glasby, Christopher J Shu-Chien, Alexander C Monroig, Óscar |
| author_facet | Bainour, Khalida Zulkifli, Nabilah Sam, Ka-Kei Navarro, Juan C Castro, Luis Filipe C Glasby, Christopher J Shu-Chien, Alexander C Monroig, Óscar Bainour, Khalida Zulkifli, Nabilah Sam, Ka-Kei Navarro, Juan C Castro, Luis Filipe C Glasby, Christopher J Shu-Chien, Alexander C Monroig, Óscar |
| collection | PubMed - marine biology |
| contents | Freshwater-adapted polychaetes exhibit a complete enzymatic machinery for synthesizing long-chain polyunsaturated fatty acids. Bainour, Khalida Zulkifli, Nabilah Sam, Ka-Kei Navarro, Juan C Castro, Luis Filipe C Glasby, Christopher J Shu-Chien, Alexander C Monroig, Óscar Animals Polychaeta Fatty Acids, Unsaturated Fatty Acid Elongases Fresh Water Fatty Acid Desaturases Phylogeny The sustainability of aquaculture is challenged by limited fishmeal and fish oil supplies, key sources of long-chain polyunsaturated fatty acids (LC-PUFA) such as eicosapentaenoic acid (EPA, 20:5 n-3), docosahexaenoic acid (DHA, 22:6 n-3) and arachidonic acid (ARA, 20:4 n-6), essential for fish health and product quality. Polychaetes represent a promising alternative. While marine polychaetes show complete LC-PUFA biosynthetic pathways involving elongases (Elovl), front-end desaturases (Fed), and methyl-end desaturases (ω des), freshwater species remain poorly studied. We hypothesize that freshwater-adapted polychaetes exhibit enhanced LC-PUFA biosynthesis to compensate for limited dietary sources in freshwater environments. This study focuses on , a freshwater nereid polychaete found in Southeast Asia. We isolated and characterized elongase and desaturase genes from using a yeast-based heterologous expression system. Our results revealed three Elovl (Elovl2/5, Elovl4, Elovl1/7) that elongate PUFA substrates from C to C, two Fed (Fed1 with Δ5 and Fed2 with dual Δ6/Δ8 activities), and two ω des: a Δ12 desaturase enabling linoleic acid (18:2 n-6) synthesis, and an ω3 desaturase converting n-6 into n-3 PUFA. These findings indicate that has the enzymatic capacity to synthesize LC-PUFA like ARA and EPA, supporting its potential for sustainable biomass production using low-nutrient substrates. |
| format | Artículo científico |
| id | pubmed_40898956 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Open biology |
| record_format | pubmed |
| spellingShingle | Freshwater-adapted polychaetes exhibit a complete enzymatic machinery for synthesizing long-chain polyunsaturated fatty acids. Bainour, Khalida Zulkifli, Nabilah Sam, Ka-Kei Navarro, Juan C Castro, Luis Filipe C Glasby, Christopher J Shu-Chien, Alexander C Monroig, Óscar Animals Polychaeta Fatty Acids, Unsaturated Fatty Acid Elongases Fresh Water Fatty Acid Desaturases Phylogeny Freshwater-adapted polychaetes exhibit a complete enzymatic machinery for synthesizing long-chain polyunsaturated fatty acids. Bainour, Khalida Zulkifli, Nabilah Sam, Ka-Kei Navarro, Juan C Castro, Luis Filipe C Glasby, Christopher J Shu-Chien, Alexander C Monroig, Óscar Animals Polychaeta Fatty Acids, Unsaturated Fatty Acid Elongases Fresh Water Fatty Acid Desaturases Phylogeny The sustainability of aquaculture is challenged by limited fishmeal and fish oil supplies, key sources of long-chain polyunsaturated fatty acids (LC-PUFA) such as eicosapentaenoic acid (EPA, 20:5 n-3), docosahexaenoic acid (DHA, 22:6 n-3) and arachidonic acid (ARA, 20:4 n-6), essential for fish health and product quality. Polychaetes represent a promising alternative. While marine polychaetes show complete LC-PUFA biosynthetic pathways involving elongases (Elovl), front-end desaturases (Fed), and methyl-end desaturases (ω des), freshwater species remain poorly studied. We hypothesize that freshwater-adapted polychaetes exhibit enhanced LC-PUFA biosynthesis to compensate for limited dietary sources in freshwater environments. This study focuses on , a freshwater nereid polychaete found in Southeast Asia. We isolated and characterized elongase and desaturase genes from using a yeast-based heterologous expression system. Our results revealed three Elovl (Elovl2/5, Elovl4, Elovl1/7) that elongate PUFA substrates from C to C, two Fed (Fed1 with Δ5 and Fed2 with dual Δ6/Δ8 activities), and two ω des: a Δ12 desaturase enabling linoleic acid (18:2 n-6) synthesis, and an ω3 desaturase converting n-6 into n-3 PUFA. These findings indicate that has the enzymatic capacity to synthesize LC-PUFA like ARA and EPA, supporting its potential for sustainable biomass production using low-nutrient substrates. |
| title | Freshwater-adapted polychaetes exhibit a complete enzymatic machinery for synthesizing long-chain polyunsaturated fatty acids. |
| topic | Animals Polychaeta Fatty Acids, Unsaturated Fatty Acid Elongases Fresh Water Fatty Acid Desaturases Phylogeny |
| url | https://pubmed.ncbi.nlm.nih.gov/40898956/ |