Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Artículo científico |
| Language: | en |
| Published: |
ISME communications
2025
|
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/40904541/ |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1868266157469859841 |
|---|---|
| author | Martinez-Garcia, Manuel Lluesma-Gomez, Monica Perez-Martin, Laura Rubio-Portillo, Esther Martin-Cuadrado, Ana Belen Nadal-Molero, Francisco Escolano-Vico, Aitana Sanchez, Fernando Santos Orphan, Victoria Antón, Josefa |
| author_facet | Martinez-Garcia, Manuel Lluesma-Gomez, Monica Perez-Martin, Laura Rubio-Portillo, Esther Martin-Cuadrado, Ana Belen Nadal-Molero, Francisco Escolano-Vico, Aitana Sanchez, Fernando Santos Orphan, Victoria Antón, Josefa Martinez-Garcia, Manuel Lluesma-Gomez, Monica Perez-Martin, Laura Rubio-Portillo, Esther Martin-Cuadrado, Ana Belen Nadal-Molero, Francisco Escolano-Vico, Aitana Sanchez, Fernando Santos Orphan, Victoria Antón, Josefa |
| collection | PubMed - marine biology |
| contents | Targeted genomic analysis of a predominant uncultured marine pelagiphage-host model via microfluidics and semipermeable capsule technology. Martinez-Garcia, Manuel Lluesma-Gomez, Monica Perez-Martin, Laura Rubio-Portillo, Esther Martin-Cuadrado, Ana Belen Nadal-Molero, Francisco Escolano-Vico, Aitana Sanchez, Fernando Santos Orphan, Victoria Antón, Josefa Microbes and their viruses drive central biogeochemical cycles on a global scale. Understanding the biology and ecology of virus-host interactions and their impact on ecosystems depends on our ability to develop tools that enable high-throughput screening of ecologically relevant, uncultured virus-host pairs. Viruses infecting Pelagibacterales, the predominant bacteria in surface oceans, have been studied through computational analyses and cultivation efforts. Here, we employ an accessible microfluidics and semi-permeable capsule (SPC) technology to investigate the uncultured pelagiphage vSAG 37-F6-host interactions since it is one of the most abundant and ubiquitous viruses in the marine virosphere. First, we validated this technology using cultured virus-host pairs. Then, marine single cells were microfluidically encapsulated in SPCs, lysed, whole-genome amplified, and screened using fluorescent polymerase chain reaction (PCR) for the presence of a hallmark gene of vSAG 37-F6. Data indicate that ~30% of the targeted cell population (cell fraction ≤0.45 μm) contained the virus vSAG 37-F6-like. A total of ~500 putatively infected cells were sorted, combined, and sequenced. Data showed that most reads (~60%) and assembled genome fragments (~85%) were identified as viral, indicating that the sorted host cells were likely in the final stages of infection. Two major viral clusters were detected: one corresponding to vSAG 37-F6 and another mixed viral cluster consisting of cyanophages, pelagiphages, and vibriophages. A significant proportion of total reads (~20%) were assigned to spp. TMED287, a bacterium reported to be abundant in the Mediterranean Sea. This flexible microfluidic-SPC technology holds enormous potential for exploring uncultured microbial and viral communities across various perspectives and microbiology fields. |
| format | Artículo científico |
| id | pubmed_40904541 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | ISME communications |
| record_format | pubmed |
| spellingShingle | Targeted genomic analysis of a predominant uncultured marine pelagiphage-host model via microfluidics and semipermeable capsule technology. Martinez-Garcia, Manuel Lluesma-Gomez, Monica Perez-Martin, Laura Rubio-Portillo, Esther Martin-Cuadrado, Ana Belen Nadal-Molero, Francisco Escolano-Vico, Aitana Sanchez, Fernando Santos Orphan, Victoria Antón, Josefa Targeted genomic analysis of a predominant uncultured marine pelagiphage-host model via microfluidics and semipermeable capsule technology. Martinez-Garcia, Manuel Lluesma-Gomez, Monica Perez-Martin, Laura Rubio-Portillo, Esther Martin-Cuadrado, Ana Belen Nadal-Molero, Francisco Escolano-Vico, Aitana Sanchez, Fernando Santos Orphan, Victoria Antón, Josefa Microbes and their viruses drive central biogeochemical cycles on a global scale. Understanding the biology and ecology of virus-host interactions and their impact on ecosystems depends on our ability to develop tools that enable high-throughput screening of ecologically relevant, uncultured virus-host pairs. Viruses infecting Pelagibacterales, the predominant bacteria in surface oceans, have been studied through computational analyses and cultivation efforts. Here, we employ an accessible microfluidics and semi-permeable capsule (SPC) technology to investigate the uncultured pelagiphage vSAG 37-F6-host interactions since it is one of the most abundant and ubiquitous viruses in the marine virosphere. First, we validated this technology using cultured virus-host pairs. Then, marine single cells were microfluidically encapsulated in SPCs, lysed, whole-genome amplified, and screened using fluorescent polymerase chain reaction (PCR) for the presence of a hallmark gene of vSAG 37-F6. Data indicate that ~30% of the targeted cell population (cell fraction ≤0.45 μm) contained the virus vSAG 37-F6-like. A total of ~500 putatively infected cells were sorted, combined, and sequenced. Data showed that most reads (~60%) and assembled genome fragments (~85%) were identified as viral, indicating that the sorted host cells were likely in the final stages of infection. Two major viral clusters were detected: one corresponding to vSAG 37-F6 and another mixed viral cluster consisting of cyanophages, pelagiphages, and vibriophages. A significant proportion of total reads (~20%) were assigned to spp. TMED287, a bacterium reported to be abundant in the Mediterranean Sea. This flexible microfluidic-SPC technology holds enormous potential for exploring uncultured microbial and viral communities across various perspectives and microbiology fields. |
| title | Targeted genomic analysis of a predominant uncultured marine pelagiphage-host model via microfluidics and semipermeable capsule technology. |
| url | https://pubmed.ncbi.nlm.nih.gov/40904541/ |