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| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Nature microbiology
2025
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/40908369/ |
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| author | Fujiwara, Takayoshi Hosaka, Toshiaki Hasegawa-Takano, Masumi Nishimura, Yosuke Tominaga, Kento Mori, Kaho Nishino, Satoshi Takahashi, Yuno Uchikubo-Kamo, Tomomi Hanada, Kazuharu Maoka, Takashi Takaichi, Shinichi Inoue, Keiichi Shirouzu, Mikako Yoshizawa, Susumu |
| author_facet | Fujiwara, Takayoshi Hosaka, Toshiaki Hasegawa-Takano, Masumi Nishimura, Yosuke Tominaga, Kento Mori, Kaho Nishino, Satoshi Takahashi, Yuno Uchikubo-Kamo, Tomomi Hanada, Kazuharu Maoka, Takashi Takaichi, Shinichi Inoue, Keiichi Shirouzu, Mikako Yoshizawa, Susumu Fujiwara, Takayoshi Hosaka, Toshiaki Hasegawa-Takano, Masumi Nishimura, Yosuke Tominaga, Kento Mori, Kaho Nishino, Satoshi Takahashi, Yuno Uchikubo-Kamo, Tomomi Hanada, Kazuharu Maoka, Takashi Takaichi, Shinichi Inoue, Keiichi Shirouzu, Mikako Yoshizawa, Susumu |
| collection | PubMed - marine biology |
| contents | Carotenoids bind rhodopsins and act as photocycle-accelerating pigments in marine Bacteroidota. Fujiwara, Takayoshi Hosaka, Toshiaki Hasegawa-Takano, Masumi Nishimura, Yosuke Tominaga, Kento Mori, Kaho Nishino, Satoshi Takahashi, Yuno Uchikubo-Kamo, Tomomi Hanada, Kazuharu Maoka, Takashi Takaichi, Shinichi Inoue, Keiichi Shirouzu, Mikako Yoshizawa, Susumu Carotenoids Bacteroidetes Rhodopsins, Microbial Rhodopsin Cryoelectron Microscopy Aquatic Organisms Seawater Light Energy Transfer Protein Binding Bacterial Proteins Microbial rhodopsins are photoreceptor proteins widely distributed in marine microorganisms that harness light energy and support marine ecosystems. While retinal is typically the sole chromophore in microbial rhodopsins, some proteorhodopsins, which are proton-pumping rhodopsins abundant in the ocean, use carotenoid antennae to transfer light energy to retinal. However, the mechanism by which carotenoids enhance rhodopsin functions remains unclear. Here, using the marine Bacteroidota isolate Nonlabens marinus S1-08, we reconstituted complexes of rhodopsins with the carotenoid myxol and detected energy transfer to retinal in both proteorhodopsin and chloride ion-pumping rhodopsin. Carotenoid binding facilitated light harvesting and accelerated the photocycle, thereby improving the light utilization efficiency of proteorhodopsin. Cryogenic electron microscopy structural analysis further revealed the molecular architecture of the carotenoid-rhodopsin complexes. The ability to bind carotenoids is conserved in rhodopsins of the marine-dominant phylum Bacteroidota, which are widely transcribed in the photic zone. These findings reveal how carotenoids enhance rhodopsin functions in marine Bacteroidota. |
| format | Artículo científico |
| id | pubmed_40908369 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Nature microbiology |
| record_format | pubmed |
| spellingShingle | Carotenoids bind rhodopsins and act as photocycle-accelerating pigments in marine Bacteroidota. Fujiwara, Takayoshi Hosaka, Toshiaki Hasegawa-Takano, Masumi Nishimura, Yosuke Tominaga, Kento Mori, Kaho Nishino, Satoshi Takahashi, Yuno Uchikubo-Kamo, Tomomi Hanada, Kazuharu Maoka, Takashi Takaichi, Shinichi Inoue, Keiichi Shirouzu, Mikako Yoshizawa, Susumu Carotenoids Bacteroidetes Rhodopsins, Microbial Rhodopsin Cryoelectron Microscopy Aquatic Organisms Seawater Light Energy Transfer Protein Binding Bacterial Proteins Carotenoids bind rhodopsins and act as photocycle-accelerating pigments in marine Bacteroidota. Fujiwara, Takayoshi Hosaka, Toshiaki Hasegawa-Takano, Masumi Nishimura, Yosuke Tominaga, Kento Mori, Kaho Nishino, Satoshi Takahashi, Yuno Uchikubo-Kamo, Tomomi Hanada, Kazuharu Maoka, Takashi Takaichi, Shinichi Inoue, Keiichi Shirouzu, Mikako Yoshizawa, Susumu Carotenoids Bacteroidetes Rhodopsins, Microbial Rhodopsin Cryoelectron Microscopy Aquatic Organisms Seawater Light Energy Transfer Protein Binding Bacterial Proteins Microbial rhodopsins are photoreceptor proteins widely distributed in marine microorganisms that harness light energy and support marine ecosystems. While retinal is typically the sole chromophore in microbial rhodopsins, some proteorhodopsins, which are proton-pumping rhodopsins abundant in the ocean, use carotenoid antennae to transfer light energy to retinal. However, the mechanism by which carotenoids enhance rhodopsin functions remains unclear. Here, using the marine Bacteroidota isolate Nonlabens marinus S1-08, we reconstituted complexes of rhodopsins with the carotenoid myxol and detected energy transfer to retinal in both proteorhodopsin and chloride ion-pumping rhodopsin. Carotenoid binding facilitated light harvesting and accelerated the photocycle, thereby improving the light utilization efficiency of proteorhodopsin. Cryogenic electron microscopy structural analysis further revealed the molecular architecture of the carotenoid-rhodopsin complexes. The ability to bind carotenoids is conserved in rhodopsins of the marine-dominant phylum Bacteroidota, which are widely transcribed in the photic zone. These findings reveal how carotenoids enhance rhodopsin functions in marine Bacteroidota. |
| title | Carotenoids bind rhodopsins and act as photocycle-accelerating pigments in marine Bacteroidota. |
| topic | Carotenoids Bacteroidetes Rhodopsins, Microbial Rhodopsin Cryoelectron Microscopy Aquatic Organisms Seawater Light Energy Transfer Protein Binding Bacterial Proteins |
| url | https://pubmed.ncbi.nlm.nih.gov/40908369/ |