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| Main Authors: | , , , , , , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Genome biology
2025
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/40913227/ |
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| _version_ | 1868266154892460032 |
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| author | Chen, Xiaona Yang, Feng Zhang, Suyang Guo, Xiaofan Zhao, Jieyu Qiao, Yulong He, Liangqiang Li, Yang Zhou, Qin Ong, Michael Tim-Yun Kwok, Chun Kit Sun, Hao Wang, Huating |
| author_facet | Chen, Xiaona Yang, Feng Zhang, Suyang Guo, Xiaofan Zhao, Jieyu Qiao, Yulong He, Liangqiang Li, Yang Zhou, Qin Ong, Michael Tim-Yun Kwok, Chun Kit Sun, Hao Wang, Huating Chen, Xiaona Yang, Feng Zhang, Suyang Guo, Xiaofan Zhao, Jieyu Qiao, Yulong He, Liangqiang Li, Yang Zhou, Qin Ong, Michael Tim-Yun Kwok, Chun Kit Sun, Hao Wang, Huating |
| collection | PubMed - marine biology |
| contents | DNA G-quadruplex profiling in skeletal muscle stem cells reveals functional and mechanistic insights. Chen, Xiaona Yang, Feng Zhang, Suyang Guo, Xiaofan Zhao, Jieyu Qiao, Yulong He, Liangqiang Li, Yang Zhou, Qin Ong, Michael Tim-Yun Kwok, Chun Kit Sun, Hao Wang, Huating G-Quadruplexes Muscle Fibers, Skeletal Myoblasts, Skeletal Cell Lineage Muscle Development Transcription, Genetic Gene Expression Regulation, Developmental Humans Animals Mice Mice, Inbred C57BL Male Female HEK293 Cells Regeneration Gene Expression Profiling DNA G-quadruplexes (G4s) are non-canonical secondary structures formed in guanine-rich DNA sequences and play important roles in modulating biological processes through a variety of gene regulatory mechanisms. Emerging G4 profiling allows global mapping of endogenous G4 formation. Here in this study, we map the G4 landscapes in adult skeletal muscle stem cells (MuSCs), which are essential for injury-induced muscle regeneration. Throughout the myogenic lineage progression of MuSCs, we uncover dynamic endogenous G4 formation with a pronounced G4 induction when MuSCs become activated and proliferating. We further demonstrate that the G4 induction promotes MuSC activation thus the regeneration process. Mechanistically, we found that promoter-associated G4s regulate gene transcription through facilitating chromatin looping. Furthermore, we found that G4 sites are enriched for transcription factor (TF) binding events in activated MuSCs; MAX binds to G4 structures to synergistically facilitate chromatin looping and gene transcription, thus promoting MuSC activation and regeneration. The above uncovered global regulatory functions/mechanisms are further dissected on the paradigm of Ccne1 promoter, demonstrating that Ccne1 is a bona fide G4/MAX regulatory target in activated MuSCs. Altogether, our findings for the first time demonstrate the prevalent and dynamic formation of G4s in adult MuSCs and the mechanistic role of G4s in modulating gene expression and MuSC activation/proliferation. |
| format | Artículo científico |
| id | pubmed_40913227 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Genome biology |
| record_format | pubmed |
| spellingShingle | DNA G-quadruplex profiling in skeletal muscle stem cells reveals functional and mechanistic insights. Chen, Xiaona Yang, Feng Zhang, Suyang Guo, Xiaofan Zhao, Jieyu Qiao, Yulong He, Liangqiang Li, Yang Zhou, Qin Ong, Michael Tim-Yun Kwok, Chun Kit Sun, Hao Wang, Huating G-Quadruplexes Muscle Fibers, Skeletal Myoblasts, Skeletal Cell Lineage Muscle Development Transcription, Genetic Gene Expression Regulation, Developmental Humans Animals Mice Mice, Inbred C57BL Male Female HEK293 Cells Regeneration Gene Expression Profiling DNA G-quadruplex profiling in skeletal muscle stem cells reveals functional and mechanistic insights. Chen, Xiaona Yang, Feng Zhang, Suyang Guo, Xiaofan Zhao, Jieyu Qiao, Yulong He, Liangqiang Li, Yang Zhou, Qin Ong, Michael Tim-Yun Kwok, Chun Kit Sun, Hao Wang, Huating G-Quadruplexes Muscle Fibers, Skeletal Myoblasts, Skeletal Cell Lineage Muscle Development Transcription, Genetic Gene Expression Regulation, Developmental Humans Animals Mice Mice, Inbred C57BL Male Female HEK293 Cells Regeneration Gene Expression Profiling DNA G-quadruplexes (G4s) are non-canonical secondary structures formed in guanine-rich DNA sequences and play important roles in modulating biological processes through a variety of gene regulatory mechanisms. Emerging G4 profiling allows global mapping of endogenous G4 formation. Here in this study, we map the G4 landscapes in adult skeletal muscle stem cells (MuSCs), which are essential for injury-induced muscle regeneration. Throughout the myogenic lineage progression of MuSCs, we uncover dynamic endogenous G4 formation with a pronounced G4 induction when MuSCs become activated and proliferating. We further demonstrate that the G4 induction promotes MuSC activation thus the regeneration process. Mechanistically, we found that promoter-associated G4s regulate gene transcription through facilitating chromatin looping. Furthermore, we found that G4 sites are enriched for transcription factor (TF) binding events in activated MuSCs; MAX binds to G4 structures to synergistically facilitate chromatin looping and gene transcription, thus promoting MuSC activation and regeneration. The above uncovered global regulatory functions/mechanisms are further dissected on the paradigm of Ccne1 promoter, demonstrating that Ccne1 is a bona fide G4/MAX regulatory target in activated MuSCs. Altogether, our findings for the first time demonstrate the prevalent and dynamic formation of G4s in adult MuSCs and the mechanistic role of G4s in modulating gene expression and MuSC activation/proliferation. |
| title | DNA G-quadruplex profiling in skeletal muscle stem cells reveals functional and mechanistic insights. |
| topic | G-Quadruplexes Muscle Fibers, Skeletal Myoblasts, Skeletal Cell Lineage Muscle Development Transcription, Genetic Gene Expression Regulation, Developmental Humans Animals Mice Mice, Inbred C57BL Male Female HEK293 Cells Regeneration Gene Expression Profiling |
| url | https://pubmed.ncbi.nlm.nih.gov/40913227/ |