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| Autores principales: | , , , , |
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| Formato: | Artículo científico |
| Lenguaje: | en |
| Publicado: |
Organic letters
2025
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| Materias: | |
| Acceso en línea: | https://pubmed.ncbi.nlm.nih.gov/40914933/ |
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| _version_ | 1868266154889314304 |
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| author | Chen, Zhenshan Duan, He Zhao, Mengqi Xiao, Fei Li, Wenli |
| author_facet | Chen, Zhenshan Duan, He Zhao, Mengqi Xiao, Fei Li, Wenli Chen, Zhenshan Duan, He Zhao, Mengqi Xiao, Fei Li, Wenli |
| collection | PubMed - marine biology |
| contents | Discovery and Characterization of a Single-Component Halogenase for Phenazine Halogenation. Chen, Zhenshan Duan, He Zhao, Mengqi Xiao, Fei Li, Wenli Phenazines Halogenation Molecular Structure Oxidoreductases Models, Molecular Biocatalysis Halogenated phenazines hold promise as antimicrobial and antibiofilm agents, yet are mainly accessed via chemical synthesis. Herein, we report PezW, a novel single-component flavin-dependent halogenase (FDH) that halogenates phenazine scaffolds, notably enabling enzymatic synthesis of bioactive 2-bromo-1-hydroxyphenazine () and 2,4-bromo-1-hydroxyphenazine (). Structural modeling and mutagenesis revealed key residues critical for substrate binding and catalysis. These findings highlight PezW's potential as a biocatalyst for the synthesis of halogenated phenazines with pharmaceutical relevance. |
| format | Artículo científico |
| id | pubmed_40914933 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Organic letters |
| record_format | pubmed |
| spellingShingle | Discovery and Characterization of a Single-Component Halogenase for Phenazine Halogenation. Chen, Zhenshan Duan, He Zhao, Mengqi Xiao, Fei Li, Wenli Phenazines Halogenation Molecular Structure Oxidoreductases Models, Molecular Biocatalysis Discovery and Characterization of a Single-Component Halogenase for Phenazine Halogenation. Chen, Zhenshan Duan, He Zhao, Mengqi Xiao, Fei Li, Wenli Phenazines Halogenation Molecular Structure Oxidoreductases Models, Molecular Biocatalysis Halogenated phenazines hold promise as antimicrobial and antibiofilm agents, yet are mainly accessed via chemical synthesis. Herein, we report PezW, a novel single-component flavin-dependent halogenase (FDH) that halogenates phenazine scaffolds, notably enabling enzymatic synthesis of bioactive 2-bromo-1-hydroxyphenazine () and 2,4-bromo-1-hydroxyphenazine (). Structural modeling and mutagenesis revealed key residues critical for substrate binding and catalysis. These findings highlight PezW's potential as a biocatalyst for the synthesis of halogenated phenazines with pharmaceutical relevance. |
| title | Discovery and Characterization of a Single-Component Halogenase for Phenazine Halogenation. |
| topic | Phenazines Halogenation Molecular Structure Oxidoreductases Models, Molecular Biocatalysis |
| url | https://pubmed.ncbi.nlm.nih.gov/40914933/ |