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| Main Authors: | , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Electrophoresis
2025
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/40916637/ |
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| _version_ | 1868266154887217152 |
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| author | Wang, Qingqing Ye, Junlan Wang, Wei |
| author_facet | Wang, Qingqing Ye, Junlan Wang, Wei Wang, Qingqing Ye, Junlan Wang, Wei |
| collection | PubMed - marine biology |
| contents | Study of Bacteriostasis of Kaempferide on Foodborne Pathogenic Bacteria by Indirect Determination of Capillary Electrophoresis. Wang, Qingqing Ye, Junlan Wang, Wei Electrophoresis, Capillary Anti-Bacterial Agents Staphylococcus aureus Vibrio parahaemolyticus Food Microbiology Kaempferols Escherichia coli Bacteria Microbial Sensitivity Tests Plant Extracts Foodborne pathogenic bacteria always threaten human health. Flavonoids are commonly used in antibacterial applications. Studying the antibacterial effect of flavonoids on bacteria is significant. Capillary electrophoresis is a versatile separation means. It has different separation modes, so, it can be applied in the analysis of both bacteria and flavonoids. In this study, an efficient method of indirect separation and detection of three bacteria Vibrio parahaemolyticus, Escherichia coli, and Staphylococcus aureus was developed. The determination of bacteria was realized by separating the aptamers corresponding to the bacteria by capillary sieving electrophoresis. This method avoided the disadvantages of low resolution, channel adsorption and bacterial aggregation, encountered in the direct separation of bacteria. The separation of the flavonoid kaempferide was realized by capillary zone electrophoresis, and kaempferide in Kaempferia galanga was determined. Kaempferide and the extract of Kaempferia galanga were applied in the antibacterial tests separately. Using the developed method, the bacterial growth curves and the curves of the kaempferide concentration were studied respectively. The results showed that both kaempferide and Kaempferia galanga could effectively inhibit the bacteria. The inhibitory effect on the terrestrial bacteria Escherichia coli, and Staphylococcus aureus was better than that on the marine bacterium Vibrio parahaemolyticus. |
| format | Artículo científico |
| id | pubmed_40916637 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Electrophoresis |
| record_format | pubmed |
| spellingShingle | Study of Bacteriostasis of Kaempferide on Foodborne Pathogenic Bacteria by Indirect Determination of Capillary Electrophoresis. Wang, Qingqing Ye, Junlan Wang, Wei Electrophoresis, Capillary Anti-Bacterial Agents Staphylococcus aureus Vibrio parahaemolyticus Food Microbiology Kaempferols Escherichia coli Bacteria Microbial Sensitivity Tests Plant Extracts Study of Bacteriostasis of Kaempferide on Foodborne Pathogenic Bacteria by Indirect Determination of Capillary Electrophoresis. Wang, Qingqing Ye, Junlan Wang, Wei Electrophoresis, Capillary Anti-Bacterial Agents Staphylococcus aureus Vibrio parahaemolyticus Food Microbiology Kaempferols Escherichia coli Bacteria Microbial Sensitivity Tests Plant Extracts Foodborne pathogenic bacteria always threaten human health. Flavonoids are commonly used in antibacterial applications. Studying the antibacterial effect of flavonoids on bacteria is significant. Capillary electrophoresis is a versatile separation means. It has different separation modes, so, it can be applied in the analysis of both bacteria and flavonoids. In this study, an efficient method of indirect separation and detection of three bacteria Vibrio parahaemolyticus, Escherichia coli, and Staphylococcus aureus was developed. The determination of bacteria was realized by separating the aptamers corresponding to the bacteria by capillary sieving electrophoresis. This method avoided the disadvantages of low resolution, channel adsorption and bacterial aggregation, encountered in the direct separation of bacteria. The separation of the flavonoid kaempferide was realized by capillary zone electrophoresis, and kaempferide in Kaempferia galanga was determined. Kaempferide and the extract of Kaempferia galanga were applied in the antibacterial tests separately. Using the developed method, the bacterial growth curves and the curves of the kaempferide concentration were studied respectively. The results showed that both kaempferide and Kaempferia galanga could effectively inhibit the bacteria. The inhibitory effect on the terrestrial bacteria Escherichia coli, and Staphylococcus aureus was better than that on the marine bacterium Vibrio parahaemolyticus. |
| title | Study of Bacteriostasis of Kaempferide on Foodborne Pathogenic Bacteria by Indirect Determination of Capillary Electrophoresis. |
| topic | Electrophoresis, Capillary Anti-Bacterial Agents Staphylococcus aureus Vibrio parahaemolyticus Food Microbiology Kaempferols Escherichia coli Bacteria Microbial Sensitivity Tests Plant Extracts |
| url | https://pubmed.ncbi.nlm.nih.gov/40916637/ |