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| Hauptverfasser: | , , , , , , , , , , , , , , , , |
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| Format: | Artículo científico |
| Sprache: | en |
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Cell
2025
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| Online-Zugang: | https://pubmed.ncbi.nlm.nih.gov/40930091/ |
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Inhaltsangabe:
- Culture-independent meta-pangenomics enabled by long-read metagenomics reveals associations with pediatric undernutrition. Minich, Jeremiah J Allsing, Nicholas Din, M Omar Tisza, Michael J Maleta, Kenneth McDonald, Daniel Hartwick, Nolan Mamerto, Allen Brennan, Caitriona Hansen, Lauren Shaffer, Justin Murray, Emily R Duong, Tiffany Knight, Rob Stephenson, Kevin Manary, Mark J Michael, Todd P Humans Metagenomics Gastrointestinal Microbiome Metagenome Genome-Wide Association Study Child, Preschool Child Male Infant Female Malnutrition Malawi Longitudinal Studies The human gut microbiome is linked to child malnutrition, yet traditional microbiome approaches lack resolution. We hypothesized that complete metagenome-assembled genomes (cMAGs), recovered through long-read (LR) DNA sequencing, would enable pangenome and microbial genome-wide association study (GWAS) analyses to identify microbial genetic associations with child linear growth. LR methods produced 44-64× more cMAGs per gigabase pair (Gbp) than short-read methods, with PacBio (PB) yielding the most accurate and cost-effective assemblies. In a Malawian longitudinal pediatric cohort, we generated 986 cMAGs (839 circular) from 47 samples and applied this database to an expanded set of 210 samples. Machine learning identified species predictive of linear growth. Pangenome analyses revealed microbial genetic associations with linear growth, while genome instability correlated with declining length-for-age Z score (LAZ). This resource demonstrates the power of comparing cMAGs with health trajectories and establishes a new standard for microbiome association studies.