Functional characterization of two Caspase-1 transcripts that associated with ASC and NLRC3 in apoptosis and inflammatory response in large yellow croaker Larimichthys crocea.
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| Natura: | Artículo científico |
| Lingua: | en |
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Fish & shellfish immunology
2025
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| author | Hu, Qiu Yu Han, Kun Huang Huang, Qiu Ye Cui, Wen Feng Yang, Qiao Yun Liu, Yi Dan Zhao, Shang Qi Zeng, Xian Yuan Li, Ying Zha, Wen Qi Wang, Yi Lei Jiang, Yong Hua Zou, Peng Fei |
| author_facet | Hu, Qiu Yu Han, Kun Huang Huang, Qiu Ye Cui, Wen Feng Yang, Qiao Yun Liu, Yi Dan Zhao, Shang Qi Zeng, Xian Yuan Li, Ying Zha, Wen Qi Wang, Yi Lei Jiang, Yong Hua Zou, Peng Fei Hu, Qiu Yu Han, Kun Huang Huang, Qiu Ye Cui, Wen Feng Yang, Qiao Yun Liu, Yi Dan Zhao, Shang Qi Zeng, Xian Yuan Li, Ying Zha, Wen Qi Wang, Yi Lei Jiang, Yong Hua Zou, Peng Fei |
| collection | PubMed - marine biology |
| contents | Functional characterization of two Caspase-1 transcripts that associated with ASC and NLRC3 in apoptosis and inflammatory response in large yellow croaker Larimichthys crocea. Hu, Qiu Yu Han, Kun Huang Huang, Qiu Ye Cui, Wen Feng Yang, Qiao Yun Liu, Yi Dan Zhao, Shang Qi Zeng, Xian Yuan Li, Ying Zha, Wen Qi Wang, Yi Lei Jiang, Yong Hua Zou, Peng Fei Animals Perciformes Fish Proteins Apoptosis Fish Diseases Caspase 1 Phylogeny Amino Acid Sequence Immunity, Innate Sequence Alignment Lipopolysaccharides Gene Expression Regulation Poly I-C Base Sequence Pseudomonas Infections Gene Expression Profiling Inflammation Pseudomonas RNA, Messenger DNA, Complementary As a member of the inflammatory caspases, Caspase-1 can increase the host inflammatory response against pathogen invasion and also function dominantly in apoptosis. In this study, we cloned and obtained two transcripts of Caspase-1 in large yellow croaker (Larimichthys crocea), namely Lc-Caspase-1_tv1 and Lc-Caspase-1_tv2. The ORF of Lc-Caspase-1_tv1 is 1239 bp, whereas Lc-Caspase-1_tv2 is 1167 bp in length, encoding a protein of 412 and 388 aa, and both of which contains a CARD and a CASc domain. Subcellular localization analysis showed that Lc-Caspase-1_tv1 and Lc-Caspase-1_tv2 are both cytoplasmically localized, and were widely expressed in the tested tissues/organs, with the highest expression level of Lc-Caspase-1_tv1 and Lc-Caspase-1_tv2 detected in the muscle and intestine, respectively. The expression of Lc-Caspase-1_tv1 and Lc-Caspase-1_tv2 was markedly induced by poly I:C, LPS, PGN stimulation, and Pseudomonas plecoglossicida infection. In addition, overexpression of Lc-Caspase-1_tv1 and Lc-Caspase-1_tv2 significantly induced apoptosis, and the co-expression of Lc-Caspase-1_tv2 but not Lc-Caspase-1_tv1 with Lc-ASC could significantly enhanced the apoptosis, whereas the co-expression of the Caspase-1 transcripts with Lc-NLRC3 could significantly abolished the apoptosis that mediated by Lc-NLRC3. Furthermore, both Lc-Caspase-1_tv1 and Lc-Caspase-1_tv2 could form protein complexes with Lc-ASC and Lc-NLRC3. The co-expression of Lc-Caspase-1_tv1 with Lc-ASC significantly enhanced the expression levels of IRF3, IRF7, TNF-α, and IFNd, whereas the co-expression of Lc-Caspase-1_tv2 with Lc-ASC significantly enhanced IRF3, Mx, and IFNd expression. Nevertheless, co-expression of the Caspase-1 transcripts with Lc-NLRC3 did not significantly impact expression of these immune-related molecules. These results indicate that Caspase-1 transcripts are essential for apoptosis and immune signaling regulation in teleosts. |
| format | Artículo científico |
| id | pubmed_40930202 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Fish & shellfish immunology |
| record_format | pubmed |
| spellingShingle | Functional characterization of two Caspase-1 transcripts that associated with ASC and NLRC3 in apoptosis and inflammatory response in large yellow croaker Larimichthys crocea. Hu, Qiu Yu Han, Kun Huang Huang, Qiu Ye Cui, Wen Feng Yang, Qiao Yun Liu, Yi Dan Zhao, Shang Qi Zeng, Xian Yuan Li, Ying Zha, Wen Qi Wang, Yi Lei Jiang, Yong Hua Zou, Peng Fei Animals Perciformes Fish Proteins Apoptosis Fish Diseases Caspase 1 Phylogeny Amino Acid Sequence Immunity, Innate Sequence Alignment Lipopolysaccharides Gene Expression Regulation Poly I-C Base Sequence Pseudomonas Infections Gene Expression Profiling Inflammation Pseudomonas RNA, Messenger DNA, Complementary Functional characterization of two Caspase-1 transcripts that associated with ASC and NLRC3 in apoptosis and inflammatory response in large yellow croaker Larimichthys crocea. Hu, Qiu Yu Han, Kun Huang Huang, Qiu Ye Cui, Wen Feng Yang, Qiao Yun Liu, Yi Dan Zhao, Shang Qi Zeng, Xian Yuan Li, Ying Zha, Wen Qi Wang, Yi Lei Jiang, Yong Hua Zou, Peng Fei Animals Perciformes Fish Proteins Apoptosis Fish Diseases Caspase 1 Phylogeny Amino Acid Sequence Immunity, Innate Sequence Alignment Lipopolysaccharides Gene Expression Regulation Poly I-C Base Sequence Pseudomonas Infections Gene Expression Profiling Inflammation Pseudomonas RNA, Messenger DNA, Complementary As a member of the inflammatory caspases, Caspase-1 can increase the host inflammatory response against pathogen invasion and also function dominantly in apoptosis. In this study, we cloned and obtained two transcripts of Caspase-1 in large yellow croaker (Larimichthys crocea), namely Lc-Caspase-1_tv1 and Lc-Caspase-1_tv2. The ORF of Lc-Caspase-1_tv1 is 1239 bp, whereas Lc-Caspase-1_tv2 is 1167 bp in length, encoding a protein of 412 and 388 aa, and both of which contains a CARD and a CASc domain. Subcellular localization analysis showed that Lc-Caspase-1_tv1 and Lc-Caspase-1_tv2 are both cytoplasmically localized, and were widely expressed in the tested tissues/organs, with the highest expression level of Lc-Caspase-1_tv1 and Lc-Caspase-1_tv2 detected in the muscle and intestine, respectively. The expression of Lc-Caspase-1_tv1 and Lc-Caspase-1_tv2 was markedly induced by poly I:C, LPS, PGN stimulation, and Pseudomonas plecoglossicida infection. In addition, overexpression of Lc-Caspase-1_tv1 and Lc-Caspase-1_tv2 significantly induced apoptosis, and the co-expression of Lc-Caspase-1_tv2 but not Lc-Caspase-1_tv1 with Lc-ASC could significantly enhanced the apoptosis, whereas the co-expression of the Caspase-1 transcripts with Lc-NLRC3 could significantly abolished the apoptosis that mediated by Lc-NLRC3. Furthermore, both Lc-Caspase-1_tv1 and Lc-Caspase-1_tv2 could form protein complexes with Lc-ASC and Lc-NLRC3. The co-expression of Lc-Caspase-1_tv1 with Lc-ASC significantly enhanced the expression levels of IRF3, IRF7, TNF-α, and IFNd, whereas the co-expression of Lc-Caspase-1_tv2 with Lc-ASC significantly enhanced IRF3, Mx, and IFNd expression. Nevertheless, co-expression of the Caspase-1 transcripts with Lc-NLRC3 did not significantly impact expression of these immune-related molecules. These results indicate that Caspase-1 transcripts are essential for apoptosis and immune signaling regulation in teleosts. |
| title | Functional characterization of two Caspase-1 transcripts that associated with ASC and NLRC3 in apoptosis and inflammatory response in large yellow croaker Larimichthys crocea. |
| topic | Animals Perciformes Fish Proteins Apoptosis Fish Diseases Caspase 1 Phylogeny Amino Acid Sequence Immunity, Innate Sequence Alignment Lipopolysaccharides Gene Expression Regulation Poly I-C Base Sequence Pseudomonas Infections Gene Expression Profiling Inflammation Pseudomonas RNA, Messenger DNA, Complementary |
| url | https://pubmed.ncbi.nlm.nih.gov/40930202/ |