Mapping heat stress-induced core histone post-translational modifications in .
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| Main Authors: | , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Environmental epigenetics
2025
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| _version_ | 1868266154865197056 |
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| author | Fuller, Cassandra N Mansoor, Sabrina Guzman, Santiago J Tose, Lilian Valadares Hackerott, Serena Rodriguez-Casariego, Javier Eirin-Lopez, Jose M Fernandez-Lima, Francisco |
| author_facet | Fuller, Cassandra N Mansoor, Sabrina Guzman, Santiago J Tose, Lilian Valadares Hackerott, Serena Rodriguez-Casariego, Javier Eirin-Lopez, Jose M Fernandez-Lima, Francisco Fuller, Cassandra N Mansoor, Sabrina Guzman, Santiago J Tose, Lilian Valadares Hackerott, Serena Rodriguez-Casariego, Javier Eirin-Lopez, Jose M Fernandez-Lima, Francisco |
| collection | PubMed - marine biology |
| contents | Mapping heat stress-induced core histone post-translational modifications in . Fuller, Cassandra N Mansoor, Sabrina Guzman, Santiago J Tose, Lilian Valadares Hackerott, Serena Rodriguez-Casariego, Javier Eirin-Lopez, Jose M Fernandez-Lima, Francisco Histone post-translational modifications (PTMs) participate in the dynamic regulation of chromatin structure and function, through their chemical nature and specific location within the histone sequence. Alternative analytical approaches for histone PTM studies are required to facilitate the differentiation between ubiquitously present isomers and the detection of low-abundance PTMs Here, we report a high-sensitivity bottom-up method based on nano-liquid chromatography (nLC), trapped ion mobility spectrometry (TIMS), data-dependent acquisition (DDA), parallel accumulation-serial fragmentation (PASEF), and high-resolution time-of-flight tandem mass spectrometry (ToF-MS/MS) for the analysis of histone PTMs. This method was tested in a threatened coral species, the staghorn coral , during an episode of acute thermal stress. The obtained results allowed for the identification of PTM changes in core histones involved in the coral's heat response. Compared to traditional LC-MS/MS approaches, the incorporation of TIMS and ddaPASEF MS/MS resulted in a highly specific and sensitive method with a wide dynamic range (6 orders of magnitude). This depth of analysis allows for the simultaneous measurement of low-abundance PTM signatures relative to the unmodified form. An added advantage is the ability to mass- and mobility-isolate prior to peptide sequencing, resulting in higher confidence identification of epigenetic markers associated with heat stress in corals (e.g. increased H4 4-17 with 2ac and 3ac, and decreases in H4 4-17 K12ac, K16ac, H4 K20me, and H2A K5ac, K7ac, K9ac, K12ac, K14ac, and K74ac). |
| format | Artículo científico |
| id | pubmed_40933922 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Environmental epigenetics |
| record_format | pubmed |
| spellingShingle | Mapping heat stress-induced core histone post-translational modifications in . Fuller, Cassandra N Mansoor, Sabrina Guzman, Santiago J Tose, Lilian Valadares Hackerott, Serena Rodriguez-Casariego, Javier Eirin-Lopez, Jose M Fernandez-Lima, Francisco Mapping heat stress-induced core histone post-translational modifications in . Fuller, Cassandra N Mansoor, Sabrina Guzman, Santiago J Tose, Lilian Valadares Hackerott, Serena Rodriguez-Casariego, Javier Eirin-Lopez, Jose M Fernandez-Lima, Francisco Histone post-translational modifications (PTMs) participate in the dynamic regulation of chromatin structure and function, through their chemical nature and specific location within the histone sequence. Alternative analytical approaches for histone PTM studies are required to facilitate the differentiation between ubiquitously present isomers and the detection of low-abundance PTMs Here, we report a high-sensitivity bottom-up method based on nano-liquid chromatography (nLC), trapped ion mobility spectrometry (TIMS), data-dependent acquisition (DDA), parallel accumulation-serial fragmentation (PASEF), and high-resolution time-of-flight tandem mass spectrometry (ToF-MS/MS) for the analysis of histone PTMs. This method was tested in a threatened coral species, the staghorn coral , during an episode of acute thermal stress. The obtained results allowed for the identification of PTM changes in core histones involved in the coral's heat response. Compared to traditional LC-MS/MS approaches, the incorporation of TIMS and ddaPASEF MS/MS resulted in a highly specific and sensitive method with a wide dynamic range (6 orders of magnitude). This depth of analysis allows for the simultaneous measurement of low-abundance PTM signatures relative to the unmodified form. An added advantage is the ability to mass- and mobility-isolate prior to peptide sequencing, resulting in higher confidence identification of epigenetic markers associated with heat stress in corals (e.g. increased H4 4-17 with 2ac and 3ac, and decreases in H4 4-17 K12ac, K16ac, H4 K20me, and H2A K5ac, K7ac, K9ac, K12ac, K14ac, and K74ac). |
| title | Mapping heat stress-induced core histone post-translational modifications in . |
| url | https://pubmed.ncbi.nlm.nih.gov/40933922/ |