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| Main Authors: | , , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Journal of natural products
2025
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/40940010/ |
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Table of Contents:
- Structure Determination and Biosynthesis of Dapalides A-C, Glycosylated Kahalalide F Analogues from the Marine Cyanobacterium sp. Ellis, Emma K Ióca, Laura P Liu, Jie Chen, Manyun Bruner, Steven D Ding, Yousong Paul, Valerie J Donia, Mohamed S Luesch, Hendrik Cyanobacteria Molecular Structure Glycosylation Depsipeptides Humans Animals Multigene Family Antineoplastic Agents Marine Biology Kahalalides were originally isolated from the marine mollusk and its green algal diet sp., but the true producer was revealed as the obligate bacterial symbiont Endobryopsis kahalalidefaciens, residing within sp. The most notable is kahalalide F, a broad-spectrum antitumor depsipeptide that entered the clinic but failed from lack of efficacy. We have isolated three new glycosylated analogues of kahalalide F, termed dapalides A-C (-), from a marine cyanobacterium, sp., collected from Guam. The planar structures were determined by extensive NMR coupled with mass spectrometry. Acid hydrolysis of using amino acid analysis revealed the absolute configuration of singlet and a mixture of duplicate amino acids. Metagenomic analysis unveiled a biosynthetic gene cluster (BGC) with a nonribosomal peptide synthetase (NRPS) system and downstream glycosylation enzymes, which assisted the configurational assignment through epimerization domain analysis. The discovered BGC, termed , was assigned to a high-quality metagenome-assembled genome of the sp. Dapalide A () was subjected to phenotypic bioassays and exhibited weak anticancer cytotoxicity. This discovery expands the chemical diversity of the kahalalide F family, suggests their broad ecological role across diverse organisms, and presents an intriguing case of natural product biosynthesis evolution.