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| Auteurs principaux: | , , , , , , , , , , , , , , , |
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| Format: | Artículo científico |
| Langue: | en |
| Publié: |
BMC microbiology
2025
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| Accès en ligne: | https://pubmed.ncbi.nlm.nih.gov/40954473/ |
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| author | de Jong, Ymke A Seren, Rana M Ramšak Marčeta, Vida Checa, Antonio Petursdottír, Dagbjort H Badolati, Isabella Moeckel, Claudia Ahmed Osman, Omneya Hell, Eva Huseby, Douglas L Hughes, Diarmaid Wheelock, Craig E Garcia, Sarahi L Udekwu, Klas I Qazi, Khaleda R Sverremark-Ekström, Eva |
| author_facet | de Jong, Ymke A Seren, Rana M Ramšak Marčeta, Vida Checa, Antonio Petursdottír, Dagbjort H Badolati, Isabella Moeckel, Claudia Ahmed Osman, Omneya Hell, Eva Huseby, Douglas L Hughes, Diarmaid Wheelock, Craig E Garcia, Sarahi L Udekwu, Klas I Qazi, Khaleda R Sverremark-Ekström, Eva de Jong, Ymke A Seren, Rana M Ramšak Marčeta, Vida Checa, Antonio Petursdottír, Dagbjort H Badolati, Isabella Moeckel, Claudia Ahmed Osman, Omneya Hell, Eva Huseby, Douglas L Hughes, Diarmaid Wheelock, Craig E Garcia, Sarahi L Udekwu, Klas I Qazi, Khaleda R Sverremark-Ekström, Eva |
| collection | PubMed - marine biology |
| contents | Impact of early-life human microbiota on the murine host metabolome: insights from a two-generation HMA mouse model and implications for allergic disease. de Jong, Ymke A Seren, Rana M Ramšak Marčeta, Vida Checa, Antonio Petursdottír, Dagbjort H Badolati, Isabella Moeckel, Claudia Ahmed Osman, Omneya Hell, Eva Huseby, Douglas L Hughes, Diarmaid Wheelock, Craig E Garcia, Sarahi L Udekwu, Klas I Qazi, Khaleda R Sverremark-Ekström, Eva Animals Humans Mice Metabolome Disease Models, Animal Gastrointestinal Microbiome Hypersensitivity Feces Female Infant Male Bacteria Human microbiota-associated (HMA) models are used to allow in vivo studies of the human gut microbiome and its effects on host physiology. In particular, alterations in early life microbiota have been linked to allergy development during childhood. In this study, we investigated how pools of human microbiota collected from infants with different allergy risk, thrive in mice and their offspring, as well as how they influence the host metabolome. We used a two-generation HMA mouse model in which dams were colonized with human feces from three groups of infants (n = 19, samples collected during the first 8 weeks of life). In two of the groups, all infants had a strong hereditary risk for allergic disease (n = 12), but only 6 of them developed allergy before 2 years of age. In the third group, which was used as a control, none of the infants had allergic heredity or developed allergy (n = 7). Microbiota trajectories were followed from inoculation to mouse offspring, and metabolic profiles were monitored in several intestinal organs as well as in the serum of the murine offspring. The human microbiota adapted to the murine host but still presented distinct compositional features, reflecting the original inoculated samples. These microbial differences were mirrored in the mouse offspring metabolome, with group-associated patterns in sphingolipids, acylcarnitines and tryptophan metabolites. Furthermore, the metabolic profiles of the mouse offspring aligned with those observed in fecal water preparations from the corresponding human infant fecal samples. Our findings highlight the significant impact of early-life microbiota on the host metabolome and show that our two-generation HMA model is suitable for studying microbiota‒metabolome relationships relevant to humans. The differences in microbiota‒metabolome correlations between individuals who develop or do not develop allergic disease suggest that an allergic predisposition might be more multifaceted than previously believed. |
| format | Artículo científico |
| id | pubmed_40954473 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | BMC microbiology |
| record_format | pubmed |
| spellingShingle | Impact of early-life human microbiota on the murine host metabolome: insights from a two-generation HMA mouse model and implications for allergic disease. de Jong, Ymke A Seren, Rana M Ramšak Marčeta, Vida Checa, Antonio Petursdottír, Dagbjort H Badolati, Isabella Moeckel, Claudia Ahmed Osman, Omneya Hell, Eva Huseby, Douglas L Hughes, Diarmaid Wheelock, Craig E Garcia, Sarahi L Udekwu, Klas I Qazi, Khaleda R Sverremark-Ekström, Eva Animals Humans Mice Metabolome Disease Models, Animal Gastrointestinal Microbiome Hypersensitivity Feces Female Infant Male Bacteria Impact of early-life human microbiota on the murine host metabolome: insights from a two-generation HMA mouse model and implications for allergic disease. de Jong, Ymke A Seren, Rana M Ramšak Marčeta, Vida Checa, Antonio Petursdottír, Dagbjort H Badolati, Isabella Moeckel, Claudia Ahmed Osman, Omneya Hell, Eva Huseby, Douglas L Hughes, Diarmaid Wheelock, Craig E Garcia, Sarahi L Udekwu, Klas I Qazi, Khaleda R Sverremark-Ekström, Eva Animals Humans Mice Metabolome Disease Models, Animal Gastrointestinal Microbiome Hypersensitivity Feces Female Infant Male Bacteria Human microbiota-associated (HMA) models are used to allow in vivo studies of the human gut microbiome and its effects on host physiology. In particular, alterations in early life microbiota have been linked to allergy development during childhood. In this study, we investigated how pools of human microbiota collected from infants with different allergy risk, thrive in mice and their offspring, as well as how they influence the host metabolome. We used a two-generation HMA mouse model in which dams were colonized with human feces from three groups of infants (n = 19, samples collected during the first 8 weeks of life). In two of the groups, all infants had a strong hereditary risk for allergic disease (n = 12), but only 6 of them developed allergy before 2 years of age. In the third group, which was used as a control, none of the infants had allergic heredity or developed allergy (n = 7). Microbiota trajectories were followed from inoculation to mouse offspring, and metabolic profiles were monitored in several intestinal organs as well as in the serum of the murine offspring. The human microbiota adapted to the murine host but still presented distinct compositional features, reflecting the original inoculated samples. These microbial differences were mirrored in the mouse offspring metabolome, with group-associated patterns in sphingolipids, acylcarnitines and tryptophan metabolites. Furthermore, the metabolic profiles of the mouse offspring aligned with those observed in fecal water preparations from the corresponding human infant fecal samples. Our findings highlight the significant impact of early-life microbiota on the host metabolome and show that our two-generation HMA model is suitable for studying microbiota‒metabolome relationships relevant to humans. The differences in microbiota‒metabolome correlations between individuals who develop or do not develop allergic disease suggest that an allergic predisposition might be more multifaceted than previously believed. |
| title | Impact of early-life human microbiota on the murine host metabolome: insights from a two-generation HMA mouse model and implications for allergic disease. |
| topic | Animals Humans Mice Metabolome Disease Models, Animal Gastrointestinal Microbiome Hypersensitivity Feces Female Infant Male Bacteria |
| url | https://pubmed.ncbi.nlm.nih.gov/40954473/ |