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| Auteurs principaux: | , , , , , , , , , , , , |
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| Format: | Artículo científico |
| Langue: | en |
| Publié: |
eLife
2025
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| Sujets: | |
| Accès en ligne: | https://pubmed.ncbi.nlm.nih.gov/40956701/ |
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Table des matières:
- Prophage regulation of 3313 motility and biofilm formation with implications for gut colonization dynamics in . Natarajan, Ojas Gibboney, Susanne L Young, Morgan N Lim, Shen Jean Nguyen, Felicia Pluta, Natalia Atkinson, Celine G F Liberti, Assunta Kees, Eric D Leigh, Brittany A Breitbart, Mya Gralnick, Jeffrey A Dishaw, Larry J Animals Biofilms Prophages Shewanella Gastrointestinal Microbiome Ciona Lysogens, bacteria with one or more viruses (prophages) integrated into their genomes, are abundant in the gut of animals. Prophages often influence bacterial traits; however, the influence of prophages on the gut microbiota-host immune axis in animals remains poorly understood. Here, we investigate the influence of the prophage SfPat on 3313, a persistent member of the gut microbiome of the model marine tunicate, . Establishment of a SfPat deletion mutant (ΔSfPat) reveals the influence of this prophage on bacterial physiology in vitro and during colonization of the gut. In vitro, deletion of SfPat reduces 3313 motility and swimming while increasing biofilm formation. To understand the in vivo impact of these prophage-induced changes in bacterial traits, we exposed metamorphic stage 4 juveniles to wildtype (WT) and ΔSfPat strains. During colonization, ΔSfPat localizes to overlapping and distinct areas of the gut compared to the WT strain. We examined the differential expression of various regulators of cyclic-di-GMP, a secondary signaling molecule that mediates biofilm formation and motility. The gene, which encodes a bacterial phosphodiesterase known to influence biofilm formation and motility by degrading cyclic-di-GMP, is upregulated in the WT strain but not in ΔSfPat when examined in vivo. Expression of the gut immune effector, VCBP-C, is enhanced during colonization by ΔSfPat compared to the WT strain; however, VCBP-C binding to the WT strain does not promote the excision of SfPat in an SOS-dependent pathway. Instead, VCBP-C binding significantly reduces the expression of a phage major capsid protein. Our findings suggest that SfPat influences host perception of this important colonizing commensal and highlights the significance of investigating tripartite dynamics between prophages, bacteria, and their animal hosts to better understand the gut microbiota-host immune axis.