Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Artículo científico |
| Language: | en |
| Published: |
Comparative biochemistry and physiology. Part D, Genomics & proteomics
2025
|
| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/40957237/ |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Table of Contents:
- A novel indel-based molecular sex identification system for oplegnathus fasciatus: Insights from ndc80 gene polymorphisms and aquaculture applications. Xiao, Yongshuang Xu, Pingrui Li, Jun Xiao, Zhizhong Animals Male Female INDEL Mutation Aquaculture Sex Determination Analysis Polymorphism, Genetic Fish Proteins Fishes Oplegnathus fasciatus is a commercially important marine fish species, valued both in wild fisheries and aquaculture. It possesses a multivalent sex-determination system (XXXX/XXY) and exhibits marked sexual growth dimorphism, with males demonstrating significantly faster growth rates. Sex-specific molecular markers are instrumental in advancing selective breeding strategies. In this study, whole-genome screening of O. fasciatus revealed a male-specific structural variant within an intronic region of ndc80: two insertions of 3bp and 538bp (totaling 541bp), which were consistently absent in females. To facilitate practical application, we developed a PCR-based assay using a single primer pair that amplifies a conserved region flanking the insertion site. This assay reproducibly generates distinct banding patterns: males yield two fragments (208bp and 749bp), consistent with the 541bp male-specific insertion, whereas females yield only the 208bp amplicon. This method enables efficient, high-throughput sex identification in O. fasciatus without the need for sequencing. Using standard agarose gel electrophoresis, the assay reliably distinguishes sexes through clearly divergent banding patterns. Beyond applications in selective breeding, these sex-specific markers provide critical molecular insights into the sex determination mechanisms and the genetic basis of sexual dimorphism in O. fasciatus.