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| Main Authors: | , , , , , , , , , , , , , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Natural product research
2025
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| Online Access: | https://pubmed.ncbi.nlm.nih.gov/40957447/ |
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Table of Contents:
- Integrated biological and chemical investigation of papuan marine organisms for the discovery of potential cytotoxic marine natural products. Hanif, Novriyandi Pakan, Jonathan Stefanson Lisias, Shannon Christela Sael, Wildan Bachtiar Santosa, Nurul Fajar Tyas, Trianda Ayuning Lemuel, Martinus Mario Mohamad, Kusdiantoro Sa'diah, Siti Dewi, Fitriya Nur Annisa Oluwabusola, Emmanuel Tope Tsunematsu, Yuta Tan, Lik Tong Chasanah, Ekowati Murni, Anggia de Voogd, Nicole J Jomori, Takahiro Kita, Masaki Jaspars, Marcel Tanaka, Junichi An integrated approach combining biological and chemical investigations were carried out to uncover novel cytotoxic molecules from diverse Papuan marine organisms. Of 386 marine-derived extracts, about 13% (50 extracts) showed significant toxicity in the lethality assay (LC ≤ 5 µg/mL). Analysis of the Z-factor and Z'-factor confirmed the excellent quality of the assay ( = 0.76, Z' = 0.5). Of the 50 active extracts, seven extracts from , sp., , a cyanobacterium, a sponge from family Thorectidae, and a rare sp. were further evaluated for cytotoxicity against HEK293T, H NMR spectral, LC-HR-MS/MS, feature-based molecular networking (FBMN), and chemoinformatics analyses. The chemical analyses revealed the presence of 43 known and 381 putatively new bioactive molecules. The integrated biological and chemistry approach adopted in this study is effective for prioritising important marine extracts with high probability of discovering novel cytotoxic compounds.