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| Auteurs principaux: | , , , , , , , , , |
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| Format: | Artículo científico |
| Langue: | en |
| Publié: |
Marine pollution bulletin
2026
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| Sujets: | |
| Accès en ligne: | https://pubmed.ncbi.nlm.nih.gov/40961576/ |
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| _version_ | 1868266152105345026 |
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| author | Sun, Hao Guo, Xiangrui Sun, Jing Zhou, Weiguo Yu, Zhen Li, Min Zhang, Shangqing Liu, Xuerui Zhao, Ye Zhang, Yanying |
| author_facet | Sun, Hao Guo, Xiangrui Sun, Jing Zhou, Weiguo Yu, Zhen Li, Min Zhang, Shangqing Liu, Xuerui Zhao, Ye Zhang, Yanying Sun, Hao Guo, Xiangrui Sun, Jing Zhou, Weiguo Yu, Zhen Li, Min Zhang, Shangqing Liu, Xuerui Zhao, Ye Zhang, Yanying |
| collection | PubMed - marine biology |
| contents | Metagenome assembled genomes revealed the influences of mariculture and seagrass species on the microbiomes in seawater and rhizosphere biotopes. Sun, Hao Guo, Xiangrui Sun, Jing Zhou, Weiguo Yu, Zhen Li, Min Zhang, Shangqing Liu, Xuerui Zhao, Ye Zhang, Yanying Rhizosphere Seawater Microbiota Metagenome Bacteria Alismatales Seagrasses provide critical ecosystem services, with their associated microbiomes playing vital roles in the health and adaptation of hosts. Metagenome sequencing has significantly advanced our understanding of seagrass-associated microbiomes; however, the application and interpretive reliability of metagenome-assembled genomes (MAGs) remain limited. This study presented a comparative analysis of high-quality MAGs from seawater and rhizosphere samples across five distinct marine habitats. A total of 93 dereplicated high-quality MAGs were obtained from seawater and rhizosphere samples and assigned to 5 and 11 phyla, respectively. Rhizosphere-derived MAGs were predominated by Desulfobacterota and exhibited enriched genes for carbon/nitrogen metabolism, dissimilatory sulfate reduction/oxidation, and glycopeptide antibiotic resistance. In contrast, MAGs from seawater primarily belonged to Pseudomonadota and Bacteroidota, with enriched genes in assimilatory sulfate reduction pathway and fluoroquinolone/tetracycline antibiotic resistance. Statistical analysis revealed that oyster culture had significantly elevated gene abundance of MAG functions linked to carbon metabolism, dissimilatory nitrate reduction, and nitrogen fixation processes in rhizosphere, while differences between seagrass species were minimal. Additionally, environmental factors, such as total organic carbon concentrations and particle sizes, exhibited closer interactions with functions of rhizosphere-associated MAGs compared to seawater samples. Our study provided novel insight into seagrass microbiome ecology, establishing a comparative genome-resolved framework to investigate functional adaptation of marine microbiome across distinct biotopes, and demonstrating the efficacy of MAG-based analysis for unrevealing environment-microbiome interactions in uncultured systems. These findings extend knowledge for developing microbial biomarkers of seagrass ecosystem health and offer methodological references for functional microbiome studies in coastal environments. |
| format | Artículo científico |
| id | pubmed_40961576 |
| institution | PubMed |
| language | en |
| publishDate | 2026 |
| publisher | Marine pollution bulletin |
| record_format | pubmed |
| spellingShingle | Metagenome assembled genomes revealed the influences of mariculture and seagrass species on the microbiomes in seawater and rhizosphere biotopes. Sun, Hao Guo, Xiangrui Sun, Jing Zhou, Weiguo Yu, Zhen Li, Min Zhang, Shangqing Liu, Xuerui Zhao, Ye Zhang, Yanying Rhizosphere Seawater Microbiota Metagenome Bacteria Alismatales Metagenome assembled genomes revealed the influences of mariculture and seagrass species on the microbiomes in seawater and rhizosphere biotopes. Sun, Hao Guo, Xiangrui Sun, Jing Zhou, Weiguo Yu, Zhen Li, Min Zhang, Shangqing Liu, Xuerui Zhao, Ye Zhang, Yanying Rhizosphere Seawater Microbiota Metagenome Bacteria Alismatales Seagrasses provide critical ecosystem services, with their associated microbiomes playing vital roles in the health and adaptation of hosts. Metagenome sequencing has significantly advanced our understanding of seagrass-associated microbiomes; however, the application and interpretive reliability of metagenome-assembled genomes (MAGs) remain limited. This study presented a comparative analysis of high-quality MAGs from seawater and rhizosphere samples across five distinct marine habitats. A total of 93 dereplicated high-quality MAGs were obtained from seawater and rhizosphere samples and assigned to 5 and 11 phyla, respectively. Rhizosphere-derived MAGs were predominated by Desulfobacterota and exhibited enriched genes for carbon/nitrogen metabolism, dissimilatory sulfate reduction/oxidation, and glycopeptide antibiotic resistance. In contrast, MAGs from seawater primarily belonged to Pseudomonadota and Bacteroidota, with enriched genes in assimilatory sulfate reduction pathway and fluoroquinolone/tetracycline antibiotic resistance. Statistical analysis revealed that oyster culture had significantly elevated gene abundance of MAG functions linked to carbon metabolism, dissimilatory nitrate reduction, and nitrogen fixation processes in rhizosphere, while differences between seagrass species were minimal. Additionally, environmental factors, such as total organic carbon concentrations and particle sizes, exhibited closer interactions with functions of rhizosphere-associated MAGs compared to seawater samples. Our study provided novel insight into seagrass microbiome ecology, establishing a comparative genome-resolved framework to investigate functional adaptation of marine microbiome across distinct biotopes, and demonstrating the efficacy of MAG-based analysis for unrevealing environment-microbiome interactions in uncultured systems. These findings extend knowledge for developing microbial biomarkers of seagrass ecosystem health and offer methodological references for functional microbiome studies in coastal environments. |
| title | Metagenome assembled genomes revealed the influences of mariculture and seagrass species on the microbiomes in seawater and rhizosphere biotopes. |
| topic | Rhizosphere Seawater Microbiota Metagenome Bacteria Alismatales |
| url | https://pubmed.ncbi.nlm.nih.gov/40961576/ |