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| Main Authors: | , , , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Genetics
2025
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/40973063/ |
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Table of Contents:
- Development of genetic tools for the sea anemone Aiptasia, a model system for coral biology. Renicke, Christian Swinhoe, Natalie Henderson, Catherine Meier, Emily Ling, Lorraine Keat, Geraldine L Maruyama, Shumpei Rangarajan-Paul, Maitri Pringle, John R Cleves, Phillip A Animals Sea Anemones Symbiosis Anthozoa Electroporation RNA, Messenger Dinoflagellida Plasmids Larva RNA, Small Interfering The reef-building corals can thrive in nutrient-poor waters because of the mutualistic symbiosis between the animal hosts and their photosynthetic dinoflagellate endosymbionts. This symbiosis is threatened by climate change and other anthropogenic stressors, so that a deeper mechanistic understanding of its function is not only of great basic biological interest but also crucial for developing rational approaches to coral conservation. The small sea anemone Aiptasia is an attractive model system for studies of this symbiosis but has been limited to date by a lack of effective genetic methods. Here, we describe the use of a simple electroporation protocol to introduce various genetic constructs [plasmid DNAs, mRNAs, and short-hairpin (sh) RNAs] into Aiptasia zygotes. Plasmid-based expression of reporter constructs in the resulting larvae was highly mosaic. In contrast, electroporation of mRNAs into zygotes resulted in uniform expression within the larvae, and success rates were similar when single or multiple mRNAs were introduced. The shRNAs were effective in knocking down expression of both coelectroporated mRNAs and endogenous genes. In this way, we could confirm the previously reported role of BRACHYURY in cnidarian embryonic development. In addition, we could show that knockdown of an Aiptasia homologue of the lysosomal-associated membrane protein 1 interfered with larval uptake and/or retention of a symbiosis-compatible algal strain. The ability to use Aiptasia larvae for such reverse-genetic studies should greatly enhance the power of this model system and serve as a starting point for further development of genetic tools in Aiptasia and other cnidarians.